Integration of insect sterility and insecticides for control of Glossina-morsitans-morsitans Westwood (Diptera, Glossinidae) in Tanzania 2. Methods of sterilization , transportation and release of sterilized males.

Surplus puparia from a colony of about 50 000 females of Glossina morsitans morsitans Westw. provided males for sterilisation and release in an experimental assessment of the sterile insect technique in Tanzania. Excess males were stored late in the puparial stage at 10 ± 1°C for up to four days prior to being irradiated at ambient temperatures in a nitrogen atmosphere with 118 krad in a radioactive caesium (137Cs) source. Puparia chilled at 8 ± 1°C were transported to the field release area twice weekly for manual release. At the release sites, the adults emerged synchronously within 60 min and were automatica

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