Keywords: aegypti

Changing mosquito genes, spreading bacteria: Science sees success vs dengue

C. E. Baclig,  INQUIRER.NET,  2022.
Wolbachia, according to WMP, are extremely common bacteria that occur naturally in 50 percent of insect species, including mosquitoes, fruit flies, moths, dragonflies, and butterflies. Aedes aegypti or dengue-carrying mosquitoes, however, do not normally carry Wolbachia. Studies ...
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Aedes aegypti and Ae. albopictus microbiome/virome: new strategies for controlling arboviral transmission?

M. Gómez, D. Martinez, M. Muñoz and J. D. Ramírez,  Parasites and Vectors,  15:287. 2022.
Aedes aegypti and Aedes albopictus are the main vectors of highly pathogenic viruses for humans, such as dengue (DENV), chikungunya (CHIKV), and Zika (ZIKV), which cause febrile, hemorrhagic, and neurological diseases and remain a major threat to global public health. The high ...
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The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males

Y. Zhao, B. Jin, P. Liu, X. Xiao, L. Cai, Z. Xie, L. Kong, T. Liu, W. Yang, Y. Wu, J. Gu, Z. Tu, A. A. James and X.-G. Chen,  PLOS Genetics,  18:e1010280. 2022.
Author summary Nix serves as a conserved male-determining factor in the two most important mosquito arboviral vectors, Ae. aegypti and Ae. albopictus. AaeNix alone can convert Ae. aegypti females into fertile but flightless males. AalNix has four alternative splice isoforms ...
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Establishment of Wolbachia infection in Aedes aegypti from Pakistan via embryonic microinjection and semi-field evaluation of general fitness of resultant mosquito population

M. S. Sarwar, N. Jahan, A. Ali, H. K. Yousaf and I. Munzoor,  Parasites and Vectors,  15:191. 2022.
BACKGROUND: Dengue is a mosquito-borne viral disease that is mainly spread by Aedes aegypti. It is prevalent on five continents, predominantly in tropical and sub-tropical zones across the world. Wolbachia bacteria have been extensively used in vector control strategies ...
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Reply to: Assessing the efficiency of Verily’s automated process for production and release of male Wolbachia-infected mosquitoes

J. E. Crawford, K. C. Hopkins, A. Buchman, T. Zha, P. Howell, E. Kakani, J. R. Ohm, N. Snoad, L. Upson, J. Holeman, P. Massaro, S. L. Dobson, F. S. Mulligan and B. J. White,  Nature Biotechnology,  2022.
We appreciate the comments from Bouyer et al. under their mandate as a United Nations agency program (‘to promote the safe and appropriate use of nuclear techniques and related technologies in food and agriculture’) on our paper1 . The centuries-old fight against ...
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Assessing the efficiency of Verily’s automated process for production and release of male Wolbachia-infected mosquitoes

J. Bouyer, H. Maiga and M. J. B. Vreysen,  Nature Biotechnology,  2022.
A paper by Crawford et al.1 titled ‘Efficient production of male Wolbachia-infected Aedes aegypti mosquitoes enables large-scale suppression of wild populations’ reports a mosquito control suppression trial carried out from2017 to 2018 in Fresco Country, California, USA1 . ...
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Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes

P. B. Patil, S. K. Dasgupta, K. Gorman, A. Pickl-Herk, M. Puinean, A. McKemey, B. Char, U. B. Zehr and S. R. Barwale,  PLOS Neglected Tropical Diseases,  16:e0010315. 2022.
Author summary Aedes aegypti L. species is the primary vector responsible for transmission of the dengue virus worldwide including chikungunya, yellow fever and Zika virus. The experiment presented in the manuscript represents a study undertaken to demonstrate suppression of the ...
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Mosquitoes Genetically Modified to Stop Disease Pass Early Test

L. Rapaport,  WebMD,  2022.
Genetically modified mosquitoes released in the U.S. appear to have passed an early test that suggests they might one day help reduce the population of insects that transmit infectious diseases. As part of the test, scientists released nearly 5 million genetically engineered ...
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Setting the world’s deadliest animal to self-destruct

A. Ossola,  QUARTZ,  2022.
The mosquitoes are coming - and the, hopefully, they're going. This wee, British biotech company Oxitec plans to kick off a months-long experiment in which it will release billions of Aedes aegypti mosquitoes in California and Florida. But these aren't just any mosquitoes - ...
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Genetically Modified Mosquitoes May Protect The World From Disease

J. R. Learn,  DISCOVER,  2022.
Forget lions, hippos or venomous spiders. Aedes aegypti mosquitoes may be among the deadliest wildlife in the world. Their bite is relatively harmless in normal circumstances. But many of these mosquitoes carry diseases they transmit from the blood of one host to another, ...
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Engineering a self-eliminating transgene in the yellow fever mosquito, Aedes aegypti

K. Chae, C. Dawson, C. Valentin, B. Contreras, J. Zapletal, K. M. Myles and Z. N. Adelman,  PNAS Nexus,  2022.
Promising genetics-based approaches are being developed to reduce or prevent the transmission of mosquito-vectored diseases. Less clear is how such transgenes can be removed from the environment, a concern that is particularly relevant for highly invasive gene drive transgenes. ...
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Pilot trial using mass field-releases of sterile males produced with the incompatible and sterile insect techniques as part of integrated Aedes aegypti control in Mexico

A. Martín-Park, A. Che-Mendoza, Y. Contreras-Perera, S. Pérez-Carrillo, H. Puerta-Guardo, J. Villegas-Chim, G. Guillermo-May, A. Medina-Barreiro, H. Delfín-González, R. Méndez-Vales, S. Vázquez-Narvaez, J. Palacio-Vargas, F. Correa-Morales, G. Ayora-Tal,  PLoS Negl Trop Dis,  16:e0010324. 2022.
We implemented a controlled before-and-after quasi-experimental study in two suburban localities of Yucatan (Mexico): San Pedro Chimay (SPC), which received IIT-SIT, and San Antonio Tahdzibichén used as control. Release of wAlbB Ae. aegypti males at SPC extended for 6 months ...
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A metapopulation approach to identify targets for Wolbachia-based dengue control

A. Reyna-Lara, D. Soriano-Paños, J. H. Arias-Castro, H. J. Martínez and J. Gómez-Gardeñes,  Chaos,  32:041105. 2022.
Over the last decade, the release of Wolbachia-infected Aedes aegypti into the natural habitat of this mosquito species has become the most sustainable and long-lasting technique to prevent and control vector-borne diseases, such as dengue, zika, or chikungunya. However, the ...
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Environmental factors influence the local establishment of Wolbachia in Aedes aegypti mosquitoes in two small communities in central Vietnam [version 2]

N. T. Hien, D. D. Anh, N. H. Le, N. T. Yen, T. V. Phong, V. S. Nam, T. N. Duong, N. B. Nguyen, D. T. T. Huong, L. Q. Hung, C. N. T. Trinh, N. V. Hoang, V. Q. Mai, L. T. Nghia, N. T. Dong, L. H. Tho, S. Kutcher, T. P. Hurst, J. L. Montgomery, M. Woolfit, E,  Gates Open Research,  5:147. 2022.
Background: The wMel strain of Wolbachia has been successfully introduced into Aedes aegypti mosquitoes and subsequently shown to reduce transmission of dengue and other pathogens, under both laboratory and field conditions. Here we describe the entomological outcomes of wMel ...
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Potential Adverse Effects of GE Mosquitoes Unknown

B. Giuffre,  The Epoch Times,  2022.
“Safe and sustainable.” That’s what Oxitec, a British biological pest control company, calls its genetically modified (GM) or genetically engineered (GE) mosquito pesticide product. The company claims its product is nontoxic to humans and animals and won’t harm beneficial ...
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Comparison of Ground Release and Drone-Mediated Aerial Release of Aedes aegypti Sterile Males in Southern Mexico: Efficacy and Challenges

C. F. Marina, P. Liedo, J. G. Bond, A. R. Osorio, J. Valle, R. Angulo-Kladt, Y. Gómez-Simuta, I. Fernández-Salas, A. Dor and T. Williams,  Insects,  13. 2022.
Sterile males of Aedes aegypti were released once a week for 8 weeks to evaluate the dispersal efficiency of ground and aerial drone release methods in a rural village of 26 Ha in southern Mexico. Indoor and outdoor BG-Sentinel traps were placed in 13–16 houses distributed ...
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Genetic Stability and Fitness of Aedes aegypti Red-Eye Genetic Sexing Strains With Pakistani Genomic Background for Sterile Insect Technique Applications

M. Misbah-ul-Haq, D. O. Carvalho, L. D. de la Fuente, A. A. Augustinos and K. Bourtzis,  Frontiers in Bioengineering and Biotechnology,  10. 2022.
The mosquito species Aedes aegypti is the primary transmitter of viruses that cause endemic diseases like dengue in Pakistan. It is also a cause of other vector-borne diseases like yellow fever, Zika fever, and chikungunya, which significantly impact human health worldwide. In ...
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Combining two Genetic Sexing Strains allows sorting of non-transgenic males for Aedes genetic control

C. Lutrat, M. Burckbuchler, R. P. Olmo, R. Beugnon, A. Fontaine, T. Baldet, J. Bouyer and E. Marois,  bioRxiv,  2022.03.11.483912. 2022.
Chemical control of the mosquito vectors Aedes albopictus and Aedes aegypti is costly, unsustainable, and increasingly ineffective due to the spread of insecticide resistance. The Sterile Insect Technique is an autocidal control tactic that represents a valuable alternative but ...
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GMO mosquitoes set for release in California to quell disease

M. Renda,  Courthouse News Service,  2022.
The U.S. Environmental Protection Agency approved the experimental use of genetically engineered mosquitoes in California and Florida to reduce the populations of invasive mosquitoes that carry a host of infectious diseases like Zika and dengue fever. “With mosquito and ...
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EVITA Dengue: a cluster-randomized controlled trial to EValuate the efficacy of Wolbachia-InfecTed Aedes aegypti mosquitoes in reducing the incidence of Arboviral infection in Brazil

M. H. Collins, G. E. Potter, M. D. T. Hitchings, E. Butler, M. Wiles, J. K. Kennedy, S. B. Pinto, A. B. M. Teixeira, A. Casanovas-Massana, N. G. Rouphael, G. A. Deye, C. P. Simmons, L. A. Moreira, M. L. Nogueira, D. A. T. Cummings, A. I. Ko, M. M. Teixeir,  Trials,  23:185. 2022.
BACKGROUND: Arboviruses transmitted by Aedes aegypti including dengue, Zika, and chikungunya are a major global health problem, with over 2.5 billion at risk for dengue alone. There are no licensed antivirals for these infections, and safe and effective vaccines are not yet ...
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Adult mosquito predation and potential impact on the sterile insect technique

N. S. Bimbilé Somda, H. Maïga, W. Mamai, T. Bakhoum, T. Wallner, S. B. Poda, H. Yamada and J. Bouyer,  Scientific Reports,  12:2561. 2022.
The sterile insect technique is a promising environmentally friendly method for mosquito control. This technique involves releasing laboratory-produced sterile males into a target field site, and its effectiveness may be affected by the extent of adult mosquito predation. Sterile ...
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Assessing Aedes aegypti candidate genes during viral infection and Wolbachia-mediated pathogen blocking

L. T. Sigle, M. Jones, M. Novelo, S. A. Ford, N. Urakova, K. Lymperopoulos, R. T. Sayre, Z. Xi, J. L. Rasgon and E. A. McGraw,  Insect Molecular Biology,  2022.
Abstract One approach to control dengue virus transmission is the symbiont Wolbachia, that limits viral infection in mosquitoes. Despite plans for its widespread use in Aedes aegypti, Wolbachia's mode of action remains poorly understood. Many studies suggest that the mechanism is ...
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A flavivirus-inducible gene expression system that modulates broad-spectrum antiviral activity against dengue and Zika viruses

S.-C. Weng, Y.-X. Zhou and S.-H. Shiao,  Insect Biochemistry and Molecular Biology,  142:103723. 2022.
Incidence of dengue virus (DENV) and Zika virus (ZIKV), two mosquito-borne flaviviruses, is increasing in large parts of the world. Vaccination and medication for these diseases are unsatisfactory. Here, we developed a novel antiviral approach, using a virus-inducible gene ...
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Could Sterile Aedes albopictus Male Releases Interfere with Aedes aegypti Population in Reunion Island?

H. F. Andrianjakarivony, D. Damiens, L. Marquereau, B. Gaudillat, N. Habchi-Hanriot and L.-C. Gouagna,  Insects,  13. 2022.
In Reunion Island, the feasibility of an Aedes albopictus control program using the Sterile Insect Technique (SIT) is studied. Because, in some regions, Ae. albopictus is living in sympatry with Aedes aegypti, the impact of releasing millions of sterile male Ae. albopictus on ...
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Effects of Sterile Males and Fertility of Infected Mosquitoes on Mosquito-Borne Disease Dynamics

X. L. Sun, S. Q. Liu, Y. F. Lv and Y. Z. Pei,  Bulletin of Mathematical Biology,  84:33. 2022.
By studying an infection-age structured model, we consider the effects of releasing sterile males and the fertility of infected mosquitoes on the mosquito-borne diseases transmission including the extinction of mosquitoes, the elimination and persistence of diseases. Firstly, ...
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The prince, the mayor, and the U.S. fish that ate Japan

C. Elliot,  National Geographic,  2022.
When Crown Prince Akihito visited Chicago on October 3, 1960, his sole request was to visit Shedd Aquarium. Then Mayor Richard J. Daley, an avid angler, presented the prince with a gift that he scooped with a net from one of the tanks himself: 18 bluegills, the official Illinois ...
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Introgression of the Aedes aegypti red-eye genetic sexing strains into different genomic backgrounds for sterile insect technique applications

A. A. Augustinos , K. Nikolouli , L. D. De La Fuente , M. Misbah-Ul-Haq, D. O. Carvalho and K. Bourtzis,  Frontiers in Bioengineering and Biotechnology,  2022.
Aedes aegypti is an invasive mosquito species and major vector of human arboviruses. A wide variety of control methods have been employed to combat mosquito populations. One of them is the sterile insect technique (SIT) that has recently attracted considerable research efforts ...
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Beyond the eye: Kynurenine pathway impairment causes midgut homeostasis dysfunction and survival and reproductive costs in blood-feeding mosquitoes

V. Bottino-Rojas, I. Ferreira, R. D. Nunes, X. Feng, T. B. Pham, A. Kelsey, R. Carballar-Lejarazú, V. Gantz, P. L. Oliveira and A. A. James,  Insect Biochemistry and Molecular Biology,  103720. 2022.
Insect ommochrome biosynthesis pathways metabolize tryptophan to generate eye-color pigments and naturally occurring alleles of pathway genes are useful phenotypic markers in transgenesis studies. Pleiotropic effects of mutations in some genes exert a load on both survival and ...
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Perspectives into Genetic Manipulations for Control of Dengue Vector (Aedes aegypti Linnaeus, 1762) with Reference to Progress in Indian Experiments

R. Chatterjee, S. Bhattacharya and B. K. Tyagi,  Genetically Modified and other Innovative Vector Control Technologies,  2021.
Vector-borne diseases like malaria, dengue, chikungunya, Japanese encephalitis, Zika and others claim millions of lives across the globe annually, and as such their control has become an ardent necessity. Past attempts over the decades have introduced vector control through ...
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Engineering RNA Interference-Based Dengue Virus Resistance in the Mosquito Vector Aedes aegypti: The Current Status and Future Directions

S. D. Denipitiyage, Y. I. N. S. Gunawardene, Z. Federico and R. S. Dassanayake,  Genetically Modified and other Innovative Vector Control Technologies,  2021.
Dengue is an acute, febrile disease caused by the dengue viruses (DENV) comprising four serotypes and transmitted by the mosquito vector Ae. aegypti. DENV are single-stranded, positive-sense RNA viruses of the family Flaviviridae. Dengue is declared as a current significant ...
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Oxitec wraps mosquito trials for the season

T. O'Hara,,  2021.
The private bio-tech company Oxitec has wrapped up its test release of genetically modified mosquitoes in the Florida Keys
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Genomic insertion locus and Cas9 expression in the germline affect CRISPR/Cas9-based gene drive performance in the yellow fever mosquito Aedes aegypti

W. R. Reid, J. Lin, A. E. Williams, R. Juncu, K. E. Olson and A. W. E. Franz,  bioRxiv,  2021.12.08.471839. 2021.
The yellow fever mosquito Aedes aegypti is a major vector of arthropod-borne viruses, including dengue, chikungunya, and Zika. A novel approach to mitigate arboviral infections is to generate mosquitoes refractory to infection by overexpressing antiviral effector molecules. Such ...
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In Real Life: GMOsquitoes

Newsy,  Newsy,  2021.
The release of genetically modified mosquitoes in Florida gives hope of reducing the spread of disease while causing concerns among some citizens.
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Major phase of Florida Keys GMO mosquito release complete, company says

D. Goodhue,  Miami Herald,  2021.
A British biotechnology company and the Florida Keys Mosquito Control District on Tuesday announced they have “successfully concluded” a major phase in a controversial trial project aimed at wiping out an invasive species of mosquito known to spread dangerous diseases like ...
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Science Has Given Us the Power to Undermine Nature’s Deadliest Creature: Should We Use It?

E. Herold,,  2021.
British biotech company Oxitec has engineered male mosquitoes to have a genetic "kill-switch" that could potentially crash the local population of Aedes aegypti, at least in the short-term. The modified males that are being released are intended to mate with wild females. Males ...
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High Temperature Cycles Result in Maternal Transmission and Dengue Infection Differences Between Wolbachia Strains in Aedes aegypti

M. V. Mancini, T. H. Ant, C. S. Herd, J. Martinez, S. M. Murdochy, D. D. Gingell, E. Mararo, P. C. D. Johnson and S. P. Sinkins,  mBio,  e0025021. 2021.
Environmental factors play a crucial role in the population dynamics of arthropod endosymbionts, and therefore in the deployment of Wolbachia symbionts for the control of dengue arboviruses. The potential of Wolbachia to invade, persist, and block virus transmission depends in ...
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Oxitec Announces Ground-breaking Commercial Launch of Its Friendly™ Aedes aegypti Solution in Brazil

Oxitec,  COSION,  2021.
Oxitec, the leading developer of biological solutions to control pests, announced today the landmark commercial launch of its Friendly™ Aedes aegypti solution designed specifically for use by homeowners, businesses, and communities to control the dengue-spreading Aedes aegypti ...
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Fighting Dengue Virus with Biological Weapons

Z. Ebrahim,  Inter Press Service,  2021.
For the last 11 years, he has been trying to convince both the provincial and central governments of making “billions of mosquitoes in labs”, which when released in the wild, could reduce the spread of dengue virus, but with little luck. The released genetically engineered ...
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Wolbachia goes to work in the war on mosquitoes

S. Ong,  Nature,  598:S32-s34. 2021.
There are two approaches to tackling dengue with Wolbachia. The first involves releasing only modified male mosquitoes. Since 2015, this strategy has been successfully adopted in Singapore and Guangzhou, China, and in parts of the United States, such as Miami, Texas and ...
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Sterilizing Male Mosquitoes with Gene Editing to Reduce Disease Spread

Global Biodefense Staff,  Global Biodefense,  2021.
Researchers at the Army’s Institute for Collaborative Biotechnologies and the University of California Santa Barbara used a gene editing tool known as CRISPR-Cas9 to target a specific gene tied to fertility in male mosquitoes. Researchers experimented with the Aedes aegypti ...
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Trial suppresses mosquitoes using non-GMO approach

GM Watch,  GM Watch,  2021.
In a first for the Southern Hemisphere, researchers have shown that a bacterium can successfully suppress populations of the invasive, disease-carrying Aedes aegypti mosquito that is responsible for spreading dengue, yellow fever and Zika. Published in PNAS (see abstract below), ...
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Invasive, disease-carrying Aedes aegypti mosquito sterilized with bacteria and eradicated in large-scale trial

CSIRO,  Phys Org,  2021.
In a first for the Southern Hemisphere, researchers have shown a bacteria can successfully sterilize and eradicate the invasive, disease carrying Aedes aegypti mosquito which is responsible for spreading dengue, yellow fever and Zika. The breakthrough could support the ...
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Releasing incompatible males drives strong suppression across populations of wild and Wolbachiat-carrying Aedes aegypti in Australia

N. W. Beebe, D. Pagendam, B. J. Trewin, A. Boomer, M. Bradford, A. Ford, C. Liddington, A. Bondarenco, P. J. De Barro, J. Gilchrist, C. Paton, K. M. Staunton, B. Johnson, A. J. Maynard, G. J. Devine, L. E. Hugo, G. Rasic, H. Cook, P. Massaro, N. Snoad, J.,  Proceedings of the National Academy of Sciences,  118:e2106828118. 2021.
Through replicated treatment and control experiments in northern Australia, regular releases of Aedes aegypti males infected with a Wolbachia from Aedes albopictus was shown to drive strong population suppression in mosaic populations of wild-type (no Wolbachia) and ...
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Investigation of Developmental Stage/Age, Gamma Irradiation Dose, and Temperature in Sterilization of Male Aedes aegypti (Diptera: Culicidae) in a Sterile Insect Technique Program

B. Ernawan, T. Anggraeni, S. Yusmalinar and I. Ahmad,  Journal of Medical Entomology,  59:320-327. 2021.
The sterilization process using gamma irradiation is a crucial component in a program using sterile insect technique (SIT) to control Aedes aegypti. Unfortunately, there is no efficient standard protocol for sterilizing mosquitoes that can produce a high level of ...
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Plan for California’s Genetically Modified Mosquitoes Draws Fire

T. Baltz,  Bloomberg Law,  2021.
A U.K.-based biotech company’s proposal to release genetically engineered mosquitoes in California as a disease-control measure is prompting criticism that the program could create unnatural consequences. Oxitec says it would unleash safe, non-biting male mosquitoes to reduce ...
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Mosquitoes Sterilized by CRISPR Powered Precision System

A. A. Sarkar,  Genetic Engineering & Biotechnology News,  2021.
Each year millions around the world are infected by dengue, chikungunya, and Zika viruses. The principal culprit behind the transmission of these deadly diseases is the mosquito vector, Aedes aegypti. Conventional methods of pest control have so far fallen short. To curb the ...
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New precision-guided sterile insect technique designed to control disease-spreading mosquitoes

E. Henderson,  News Medical Life Sciences,  2021.
Leveraging advancements in CRISPR-based genetic engineering, researchers at the University of California San Diego have created a new system that restrains populations of mosquitoes that infect millions each year with debilitating diseases. The new precision-guided sterile ...
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Genetic engineering tech promises to sterilize disease-spreading mosquitoes

B. Hays,  UPI,  2021.