Keywords: Biotechnology & Applied Microbiology

Cas9-triggered chain ablation of cas9 as a gene drive brake Wu, BL, L. Q.; Gao, X. J. J.,  Nature Biotechnology,  34:137-138. 2016. We designed and synthesized a transgene system that we named Cas9-triggered chain ablation (CATCHA). The CATCHA transgene encodes a guide RNA (gRNA) that is expressed ubiquitously from a U6:2 promoter. The gRNA targets a site within the DNA sequence of cas9. The guide RNA is ... Keywords: Biotechnology & Applied Microbiology, drosophila, gene drive genetics, gene drive mechanisms, gene drive safety, gene drive synthetic, synthetic homing drive, vasa