Keywords: CRISPR-Cas system
|
Homing-based gene drives can introgress rapidly into local genetic backgrounds with minimal chromosomal conversion in Anopheles gambiaeTony Nolan, Poppy Pescod, Giulia Bevivino et al., Research Square, 2023.
CRISPR-Cas9 gene drive control strategies use a homing selfish genetic element which induces a double-stranded break at the target site and is copied into the opposing chromosome, breaking an essential gene and ensuring super-Mendelian inheritance. During the copying process SNPs ... |
|
Kinetic basis for DNA target specificity of CRISPR-Cas12aStrohkendl, IS, Fatema A.; Rybarski, James R.; Finkelstein, Ilya J.; Russell, Rick, Molecular Cell, 71:816-824. 2018.
Summary Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising applications in human health and disease. However, DNA cleavage at off-target sites that resemble the target sequence is a pervasive problem that remains poorly understood mechanistically. ... |
|
Cheating evolution: engineering gene drives to manipulate the fate of wild populationsChamper, JB, A.; Akbari, O. S., Nature Reviews Genetics, 17:146-159. 2016.
Engineered gene drives - the process of stimulating the biased inheritance of specific genes - have the potential to enable the spread of desirable genes throughout wild populations or to suppress harmful species, and may be particularly useful for the control of vector-borne ... |
