Keywords: CRISPR-Cas9 ddPCR gene editing IDAA mosquitoes NHEJ quantification gene drive Biochemistry & Molecular Biology
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Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensiR. R. Carballar-Lejarazu, A. Kelsey, T. B. Pham, E. P. Bennett and A. A. James-Lejarazu, A. Kelsey, T. B. Pham, E. P. Bennett and A. A. James, Biotechniques, 68:172-179. 2020.
CRISPR/Cas9 technology is a powerful tool for the design of gene-drive systems to control and/or modify mosquito vector populations; however, CRISPR/Cas9-mediated nonhomologous end joining mutations can have an important impact on generating alleles resistant to the drive and ... |
