Keywords: funestus
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CRISPR-mediated knock-in of transgenes into the malaria vector Anopheles funestusC. Quinn, A. Anthousi, C. Wondji and T. Nolan, G3 Genes Genomes Genetics, 11. 2021.
The ability to introduce mutations, or transgenes, of choice to precise genomic locations has revolutionized our ability to understand how genes and organisms work. In many mosquito species that are vectors of various human diseases, the advent of CRISPR genome editing tools has ... |
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CRISPR-mediated knock-in of transgenes into the malaria vector Anopheles funestusC. Quinn, A. Anthousi, C. Wondji and T. Nolan, bioRxiv, 2021.03.31.437891. 2021.
We describe herein an optimised transformation system based on the germline delivery of CRISPR components that allows efficient cleavage of a previously validated genomic site and preferential repair of these cut sites via homology-directed repair (HDR), which allows introduction ... |
