Keywords: genetic sexing strain

Precise single base substitution in the shibire gene by CRISPR/Cas9-mediated homology directed repair in Bactrocera tryoni

A. Choo, E. Fung, I. Y. Chen, R. Saint, P. Crisp and S. W. Baxter,  BMC Genetics,  21. 2020.
Here we introduce a known Drosophila melanogaster temperature sensitive embryonic lethal mutation into Bactrocera tryoni, a serious horticultural pest in Australia. A non-synonymous point mutation in the D. melanogaster gene shibire causes embryonic lethality at 29 degrees C and ...
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