Keywords: Genome editing

Genome editing in pests: basic science to applications

Chen, X., Palli, S.R.,  Journal of Pest Science,  2024.
Recent developments in sequencing technologies produced enormous data on gene sequences and the identity of genes in many pest insects and disease vectors. However, the function of many of these genes is unknown. Functional genomics studies to uncover gene function in pest ...
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A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations

Anderson, M.A.E., Gonzalez, E., Edgington, M.P. et al.,  Nature Communications,  15. 2024.
Aedes aegypti is the main vector of several major pathogens including dengue, Zika and chikungunya viruses. Classical mosquito control strategies utilizing insecticides are threatened by rising resistance. This has stimulated interest in new genetic systems such as gene ...
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CRISPR/Cas9: a cutting-edge solution for combatting the fall armyworm, Spodoptera frugiperda

Gouda, M.N.R., Jeevan, H., Shashank, H.G.,  Molecular Biology Reports,  51. 2023.
The utilization of CRISPR/Cas9 in Spodoptera frugiperda, commonly known as fall armyworm, presents a groundbreaking avenue for pest management. With its ability to precisely modify the insect’s genome, CRISPR/Cas9 offers innovative strategies to combat this destructive pest. ...
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Homing-based gene drives can introgress rapidly into local genetic backgrounds with minimal chromosomal conversion in Anopheles gambiae

Tony Nolan, Poppy Pescod, Giulia Bevivino et al.,  Research Square,  2023.
CRISPR-Cas9 gene drive control strategies use a homing selfish genetic element which induces a double-stranded break at the target site and is copied into the opposing chromosome, breaking an essential gene and ensuring super-Mendelian inheritance. During the copying process SNPs ...
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Ghana publishes its guidelines on Genome Editing

Anonymous,  BusinessGhana,  2023.
Ghana's National Biosafety Authority (NBA) has published its guidelines on Genome Editing and Gene Stacks with regards Genetically Modified Organisms (GMOs) joining Nigeria, Kenya, and Malawi, as the fourth country in Africa with the guidelines. Genome editing is an emerging and ...
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Deficits of Public Deliberation in U.S. Oversight for Gene Edited Organisms

J. Kuzma,  Hastings Center Report,  51 Suppl 2:S25-s33. 2021.
Environmental releases of gene edited (GEdOs) and gene drive organisms (GDOs) will likely occur under conditions of high uncertainty and in complex socioecological systems. Therefore, public deliberation is especially important to account for diverse interpretations of safety, ...
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Gene drive: a faster route to plant improvement

H. A. Siddiqui, T. Harvey-Samuel and S. Mansoor,  Trends in Plant Science,  2021.
Gene drives for control of vector-borne diseases have been demonstrated in insects but remain challenging in plants. Theoretically, they could be transformative in speeding breeding programs and contributing to food security through providing novel weed control methods. Zhang et ...
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Ethics of Genome Editing

European Group on Ethics,  European Group on Ethics in Science and New Technologies,  2021.
This Opinion addresses the profound ethical questions raised and revived by them. It analyses various domains of application, from human health to animal experimentation, from livestock breeding to crop variety and to gene drives. With its wide view across areas, it identifies ...
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Population genomics of invasive rodents on islands: Genetic consequences of colonization and prospects for localized synthetic gene drive

K. P. Oh, A. B. Shiels, L. Shiels, D. V. Blondel, K. J. Campbell, J. R. Saah, A. L. Lloyd, P. Q. Thomas, F. Gould, Z. Abdo, J. R. Godwin and A. J. Piaggio,  Evolutionary Applications,  2021.
Here we used pooled whole-genome sequencing of invasive mouse (Mus musculus) populations on four islands along with paired putative source populations to test genetic predictions of island colonization and characterize locally fixed Cas9 genomic targets. Patterns of variation ...
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Bioethical issues in genome editing by CRISPR-Cas9 technology

F. B. Ayanoglu, A. E. Elcin and Y. M. Elcin,  Turkish Journal of Biology,  44:110-120. 2020.
Genome editing technologies have led to fundamental changes in genetic science. Among them, CRISPR-Cas9 technology particularly stands out due to its advantages such as easy handling, high accuracy, and low cost. It has made a quick introduction in fields related to humans, ...
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Genome engineering in insects: focus on the CRISPR/Cas9 system

Hillary, V. Edwin Ceasar, Stanislaus Antony Ignacimuthu, S.,  Genome Engineering via CRISPR-Cas9 System,  2020.
Genome engineering is a precise tool used to alter the genome of desired organism. Zinc finger nuclease (ZFN), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR), and the CRISPR-associated RNA guided ...
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Regulation of GM Organisms for Invasive Species Control

H. J. Mitchell and D. Bartsch,  Frontiers in Bioengineering and Biotechnology,  7:1-11. 2020.
Invasive species can cause significant harm to the environment, agriculture, and human health, but there are often very limited tools available to control their populations. Gene drives (GD) have been proposed as a new tool which could be used to control or eliminate such ...
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Improving plant-resistance to insect-pests and pathogens: The new opportunities through targeted genome editing

D. S. Bisht, V. Bhatia and R. Bhattacharya,  Seminars in Cell & Developmental Biology,  96:65-76. 2019.
The advantages of high input agriculture are fading away due to degenerating soil health and adverse effects of climate change. Safeguarding crop yields in the changing environment and dynamics of pest and pathogens, has posed new challenges to global agriculture. Thus, ...
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The End of the GMO? Genome Editing, Gene Drives and New Frontiers of Plant Technology

K. L. Hefferon and R. J. Herring,  Review of Agrarian Studies,  7. 2017.
mprovements to agriculture will constitute one of the world’s greatest challenges in the coming century. Political and social controversies, as well as complications of plant breeding, intellectual property, and regulation, have compromised the promised impact of genetically ...
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