GRNA Archives

	Split versions of Cleave and Rescue selfish genetic elements for measured self limiting gene drive G. Oberhofer, T. Ivy and B. A. Hay, PLoS genetics, 17:e1009385. 2021. Self-sustaining Cleave and Rescue (ClvR) elements include a DNA sequence-modifying enzyme such as Cas9/gRNAs that disrupts endogenous versions of an essential gene, a tightly linked recoded version of the essential gene resistant to cleavage (the Rescue), and a Cargo. ClvR ... Keywords: CRISPR, drosophila, GRNA, Homing
	A Multiple Gene Drive System Ferdinand Nanfack Minkeu, IGTRCN, 2019. Yan & Finnigan, (2018) recently published a paper in Scientific Reports describing an artificial multi-locus gene drive system by using a single Cas9 and three guide RNA (gRNA) in the budding yeast Saccharomyces cerevisiae. Nuclease-based gene drives do not follow the typical ... Keywords: CRISPR, drosophila, GRNA, Homing
	Multiplexing gRNAs to Hedge Against Resistance to Gene Drive Travis Van Warmerdam, IGTRCN, 2018. Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for ... Keywords: CRISPR, drosophila, GRNA, Homing