Keywords: homing drive

Development of CRISPR/Cas9-Mediated Gene-Drive Construct Targeting the Phenotypic Gene in Plutella xylostella

M. Asad, D. Liu, J. Li, J. Chen and G. Yang,  Frontiers in Physiology,  13:938621. 2022.
The gene-drive system can ensure that desirable traits are transmitted to the progeny more than the normal Mendelian segregation. The clustered regularly interspersed palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) mediated gene-drive system has been demonstrated ...
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Experimental demonstration of tethered gene drive systems for confined population modification or suppression

M. Metzloff, E. Yang, S. Dhole, A. G. Clark, P. W. Messer and J. Champer,  BMC Biol,  20:119. 2022.
BACKGROUND: Homing gene drives hold great promise for the genetic control of natural populations. However, current homing systems are capable of spreading uncontrollably between populations connected by even marginal levels of migration. This could represent a substantial ...
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Experimental demonstration of tethered gene drive systems for confined population modification or suppression

M. Metzloff, E. Yang, S. Dhole, A. G. Clark, P. W. Messer and J. Champer,  bioRxiv,  2021.05.29.446308. 2022.
Homing gene drives hold great promise for the genetic control of natural populations. However, current homing systems are capable of spreading uncontrollably between populations connected by even marginal levels of migration. This could represent a substantial sociopolitical ...
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Gene drive escape from resistance depends on mechanism and ecology

F. Cook, J. J. Bull and R. Gomulkiewicz,  Evolutionary Applications,  2022.
Abstract Gene drives can potentially be used to suppress pest populations, and the advent of CRISPR technology has made it feasible to engineer them in many species, especially insects. What remains largely unknown for implementations is whether antidrive resistance will evolve ...
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Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation

S. Fuchs, W. T. Garrood, A. Beber, A. Hammond, R. Galizi, M. Gribble, G. Morselli, T.-Y. J. Hui, K. Willis, N. Kranjc, A. Burt, A. Crisanti and T. Nolan,  PLOS Genetics,  17. 2021.
Author summary Gene drives have the potential to be applied as a novel control strategy of disease-transmitting mosquitoes, by spreading genetic traits that suppress or modify the target population. Many gene drive elements work by recognising and cutting a specific target ...
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Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation

S. Fuchs, W. Garrood, A. Beber, A. Hammond, R. Galizi, M. Gribble, G. Morselli, T.-Y. Hui, K. Willis, N. Kranjc, A. Burt, T. Nolan and A. Crisanti,  bioRxiv,  2021.
CRISPR-based homing gene drives can be designed to disrupt essential genes whilst biasing their own inheritance, leading to suppression of mosquito populations in the laboratory. This class of gene drives relies on CRISPR-Cas9 cleavage of a target sequence and copying ...
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Converting endogenous genes of the malaria mosquito into simple non-autonomous gene drives for population replacement

A. Hoermann, S. Tapanelli, P. Capriotti, G. Del Corsano, E. K. G. Masters, T. Habtewold, G. K. Christophides and N. Windbichler,  eLife,  10. 2021.
Gene drives for mosquito population replacement are promising tools for malaria control. However, there is currently no clear pathway for safely testing such tools in endemic countries. The lack of well-characterized promoters for infection-relevant tissues and regulatory hurdles ...
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Evading resistance to gene drives

R. Gomulkiewicz, M. L. Thies and J. J. Bull,  Genetics,  217. 2021.
Here, we develop mathematical and computational models to identify conditions under which suppression drives will evade resistance, even if resistance is present initially. Previous models assumed resistance is allelic to the drive. We relax this assumption and show that linkage ...
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CRISPR gene drive efficiency and resistance rate is highly heritable with no common genetic loci of large effect

Champer, JW, Z. X.; Luthra, A.; Reeves, R.; Chung, J.; Liu, C.; Lee, Y. L.; Liu, J. X.; Yang, E.; Messer, P. W.; Clark, A. G.,  Genetics,  212:333-341. 2019.
Gene drives could allow for control of vector-borne diseases by directly suppressing vector populations or spreading genetic payloads designed to reduce pathogen transmission. Clustered regularly interspaced short palindromic repeat (CRISPR) homing gene drives work by cleaving ...
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