Keywords: rate-limiting binding
Kinetic basis for DNA target specificity of CRISPR-Cas12aStrohkendl, IS, Fatema A.; Rybarski, James R.; Finkelstein, Ilya J.; Russell, Rick, Molecular Cell, 71:816-824. 2018.
Summary Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising applications in human health and disease. However, DNA cleavage at off-target sites that resemble the target sequence is a pervasive problem that remains poorly understood mechanistically. ...
Keywords: AsCpf1, Cpf1, CRISPR-Cas system, gene drive mechanisms, gene drive synthetic, rate-limiting binding, RNA-guided nuclease, RNA-protein enzyme, RNA/DNA assembly, RNA/DNA thermodynamics
Foundation for the
National Institutes of Health