Keywords: rate-limiting binding
Summary Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising applications in human health and disease. However, DNA cleavage at off-target sites that resemble the target sequence is a pervasive problem that remains poorly understood mechanistically. ...
Keywords: AsCpf1, Cpf1, CRISPR-Cas system, gene drive mechanisms, gene drive synthetic, rate-limiting binding, RNA-guided nuclease, RNA-protein enzyme, RNA/DNA assembly, RNA/DNA thermodynamics