Keywords: RNA-guided nuclease
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Kinetic basis for DNA target specificity of CRISPR-Cas12aStrohkendl, IS, Fatema A.; Rybarski, James R.; Finkelstein, Ilya J.; Russell, Rick, Molecular Cell, 71:816-824. 2018.
Summary Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising applications in human health and disease. However, DNA cleavage at off-target sites that resemble the target sequence is a pervasive problem that remains poorly understood mechanistically. ... |
