
Keywords: RNA-guided nuclease
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Kinetic basis for DNA target specificity of CRISPR-Cas12aStrohkendl, IS, Fatema A.; Rybarski, James R.; Finkelstein, Ilya J.; Russell, Rick, Molecular Cell, 71:816-824. 2018.![]() Summary Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising applications in human health and disease. However, DNA cleavage at off-target sites that resemble the target sequence is a pervasive problem that remains poorly understood mechanistically. ... Keywords: AsCpf1, Cpf1, CRISPR-Cas system, gene drive mechanisms, gene drive synthetic, rate-limiting binding, RNA-guided nuclease, RNA-protein enzyme, RNA/DNA assembly, RNA/DNA thermodynamics |

Contact
David O’Brochta
Foundation for the
National Institutes of Health
geneconvenevi@fnih.org
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