Keywords: RNA-protein enzyme

Kinetic basis for DNA target specificity of CRISPR-Cas12a

Strohkendl, IS, Fatema A.; Rybarski, James R.; Finkelstein, Ilya J.; Russell, Rick,  Molecular Cell,  71:816-824. 2018.
Summary Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising applications in human health and disease. However, DNA cleavage at off-target sites that resemble the target sequence is a pervasive problem that remains poorly understood mechanistically. ...
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