Keywords: saccharomyces-cerevisiae
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Developing GDi-CRISPR System for Multi-copy Integration in Saccharomyces cerevisiaeZ.-X. Zhang, Y.-Z. Wang, Y.-S. Xu, X.-M. Sun and H. Huang, Applied Biochemistry and Biotechnology, 2021.
This study aims to develop a low-cost and easy-to-use multi-copy integration tool in S. cerevisiae. Firstly, twenty-one Cas proteins from different microorganisms were tested in S. cerevisiae to find the functional Cas proteins with optimal cleavage ability. Results showed that ... |
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Vector dynamics influence spatially imperfect genetic interventions against diseaseM. K. Yuksel, C. H. Remien, B. Karki, J. J. Bull and S. M. Krone, Evolution, Medicine, and Public Health, 9:1-10. 2020.
In spatially structured populations, imperfect coverage of the vector will leave pockets in which the parasite may persist. Movement by humans may disrupt this local persistence and facilitate eradication when these pockets are small, spreading parasite reproduction outside ... |
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Chromosome drives via CRISPR-Cas9 in yeastH. Xu, M. Han, S. Zhou, B.-Z. Li, Y. Wu and Y.-J. Yuan, Nature Communications, 11:4344. 2020.
Our results show that the entire Saccharomyces cerevisiae chromosome can be eliminated efficiently through only one double-strand break around the centromere via CRISPR-Cas9. As a proof-of-concept experiment of this CRISPR-Cas9 chromosome drive system, the synthetic yeast ... |
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Rapid evolution of yeast centromeres in the absence of driveBensasson, DZ, M.; Burt, A.; Koufopanou, V., Genetics, 178:2161-2167. 2008.
To find the most rapidly evolving regions in the yeast genome we compared most of chromosome III from three closely related lineages of the wild yeast Saccharomyces paradoxits. Unexpectedly, the centromere appears to be the fastest-evolving part of the chromosome, evolving even ... |
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Evolution of divergent DNA recognition specificities in VDE homing endonucleases from two yeast speciesPosey, KLK, V.; Burt, A.; Gimble, F. S., Nucleic Acids Research, 32:3947-3956. 2004.
Homing endonuclease genes (HEGs) are mobile DNA elements that are thought to confer no benefit to their host. They encode site-specific DNA endonucleases that perpetuate the element within a species population by homing and disseminate it between species by horizontal transfer. ... |
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Homing endonuclease genes: the rise and fall and rise again of a selfish elementBurt, AK, V., Current Opinion in Genetics & Development, 14:609-615. 2004.
Homing endonuclease genes (HEGs) are selfish genetic elements that spread by first cleaving chromosomes that do not contain them and then getting copied across to the broken chromosome as a byproduct of the repair process. The success of this strategy will depend on the ... |
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Outcrossed sex allows a selfish gene to invade yeast populationsGoddard, MRG, D.; Burt, A., Proceedings of the Royal Society B-Biological Sciences, 268:2537-2542. 2001.
Homing endonuclease genes (HEGs) in eukaryotes are optional genes that have no obvious effect on host phenotype except for causing chromosomes not containing a cop), of the gene to be cut, thus causing them to be inherited at a greater than Mendelian rate via gene conversion. ... |
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Recurrent invasion and extinction of a selfish geneGoddard, MRB, A., Proceedings of the National Academy of Sciences of the United States of America, 96:13880-13885. 1999.
Homing endonuclease genes show super-Mendelian inheritance, which allows them to spread in populations even when they are of no benefit to the host organism. To test the idea that regular horizontal transmission is necessary for the long-term persistence of these genes, we ... |
