Keywords: split drive

Gene Drives Built to Follow More Stringent Rules of the Road

Anonymous,  Genetic Engineering & Biotechnology News,  2021.
Gene drives, or systems that accelerate the spread of desirable genetic traits into a population, may be built to achieve specific levels of spread when released into the wild. By exerting control over the degree of spread, those who unleash gene drives may realize the benefits ...
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New gene-drive technologies can help control crop pests

Anonymous,  AZO Life Sciences,  2021.
The supposed gene drives, which exploit CRISPR technology to affect genetic inheritance, show the potential to quickly spread particular genetic traits across the populations of a specified species. For instance, gene-drive technologies used on insects are being developed to stop ...
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Inherently confinable split-drive systems in Drosophila

G. Terradas, A. B. Buchman, J. B. Bennett, I. Shriner, J. M. Marshall, O. S. Akbari and E. Bier,  Nature Communications,  12:1480. 2021.
CRISPR-based gene-drive systems, which copy themselves via gene conversion mediated by the homology-directed repair (HDR) pathway, have the potential to revolutionize vector control. However, mutant alleles generated by the competing non-homologous end-joining (NHEJ) pathway, ...
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New ‘Split-drive’ System Puts Scientists in the (Gene) Driver Seat

M. Aguilera,  UC San Diego News Center,  2021.
Gene-drive technologies applied in insects, for example, are being designed to halt the spread of devastating diseases such as malaria and dengue by preventing mosquito hosts from becoming infected. In agricultural fields, gene-drives are being developed to help control or ...
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Split versions of Cleave and Rescue selfish genetic elements for measured self limiting gene drive

G. Oberhofer, T. Ivy and B. A. Hay,  PLoS genetics,  17:e1009385. 2021.
Self-sustaining Cleave and Rescue (ClvR) elements include a DNA sequence-modifying enzyme such as Cas9/gRNAs that disrupts endogenous versions of an essential gene, a tightly linked recoded version of the essential gene resistant to cleavage (the Rescue), and a Cargo. ClvR ...
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A CRISPR endonuclease gene drive reveals two distinct mechanisms of inheritance bias

S. A. N. Verkuijl, E. González, J. X. D. Ang, M. Li, N. P. Kandul, M. Anderson, O. S. Akbari, M. Bonsall and L. Alphey,  bioRxiv,  2020.12.15.421271. 2020.
In this study, we report the functioning of sds3, bgcn, and nup50 expressed Cas9 in an Aedes aegypti homing split drive system targeting the white gene. We report their inheritance biasing capability, propensity for maternal deposition, and zygotic/somatic expression. ...
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Split drive killer-rescue provides a novel threshold-dependent gene drive

M. P. Edgington, T. Harvey-Samuel and L. Alphey,  Scientific Reports,  10. 2020.
Population genetics mathematical models are developed here to demonstrate the threshold-dependent nature of the proposed system and its robustness to imperfect homing, incomplete penetrance of toxins and transgene fitness costs, each of which are of practical significance given ...
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Inherently confinable split-drive systems in Drosophila

G. Terradas, A. B. Buchman, J. B. Bennett, I. Shriner, J. M. Marshall, O. S. Akbari and E. Bier,  bioRxiv,  2020.09.03.282079. 2020.
Here, we test split gene-drive (sGD) systems in Drosophila melanogaster that were inserted into essential genes required for viability (rab5, rab11, prosalpha2) or fertility (spo11). I
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Assessment of a Split Homing Based Gene Drive for Efficient Knockout of Multiple Genes

N. P. Kandul, J. Liu, A. Buchman, V. M. Gantz, E. Bier and O. S. Akbari,  G3: Genes|Genomes|Genetics,  10:827-837. 2019.
Homing based gene drives (HGD) possess the potential to spread linked cargo genes into natural populations and are poised to revolutionize population control of animals. Given that host encoded genes have been identified that are important for pathogen transmission, targeting ...
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Assessment of a split homing based gene drive for efficient knockout of multiple genes

Kandul, N. P., J. Liu, A. Buchman, V. M. Gantz, E. Bier and O. S. Akbari,  bioRxiv,  2019:706929. 2019.
Homing based gene drives (HGD) possess the potential to spread linked cargo genes into natural populations and are poised to revolutionize population control of animals. Given that host-encoded genes have been identified that are important for pathogen transmission, targeting ...
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Split-gene drive system provides flexible application for safe laboratory investigation and potential field deployment

V. L. Del Amo, A. L. Bishop, H. M. Sánchez C, J. B. Bennett, X. Feng, J. M. Marshall, E. Bier and V. M. Gantz,  bioRxiv,  684597. 2019.
CRISPR-based gene drives spread through populations bypassing the dictates of Mendelian genetics, offering a population-engineering tool for tackling vector-borne diseases, managing crop pests, and helping island conservation efforts; unfortunately, current technologies raise ...
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