Keywords: temperature sensitivity
Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitataSollazzo, G., Nikolouli, K., Gouvi, G. et al., BMC Biotechnology, 24. 2024.The Mediterranean fruit fly, Ceratitis capitata, is a significant agricultural pest managed through area-wide integrated pest management (AW-IPM) including a sterile insect technique (SIT) component. Male-only releases increase the efficiency and cost-effectiveness of SIT ... Keywords: Bactrocera dorsalis, Drosophia, genetic biocontrol, genetic sexing strain, SIT, sterile insect technique, temperature sensitivity |
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Next-generation CRISPR gene-drive systems using Cas12a nucleaseS. Sanz Juste, E. M. Okamoto, X. Feng and V. L. Del Amo, bioRxiv, 2023.02.20.529271. 2023.One method for reducing the impact of vector-borne diseases is through the use of CRISPR-based gene drives, which manipulate insect populations due to their ability to rapidly propagate desired genetic traits into a target population. However, all current gene drives employ a ... Keywords: Bactrocera dorsalis, Drosophia, genetic biocontrol, genetic sexing strain, SIT, sterile insect technique, temperature sensitivity |
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Next-generation CRISPR gene-drive systems using Cas12a nucleaseS. Sanz Juste, E. M. Okamoto, X. Feng and V. L. Del Amo, bioRxiv, 2023.02.20.529271. 2023.One method for reducing the impact of vector-borne diseases is through the use of CRISPR-based gene drives, which manipulate insect populations due to their ability to rapidly propagate desired genetic traits into a target population. However, all current gene drives employ a ... Keywords: Bactrocera dorsalis, Drosophia, genetic biocontrol, genetic sexing strain, SIT, sterile insect technique, temperature sensitivity |
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Precise single base substitution in the shibire gene by CRISPR/Cas9-mediated homology directed repair in Bactrocera tryoniA. Choo, E. Fung, I. Y. Chen, R. Saint, P. Crisp and S. W. Baxter, BMC Genetics, 21. 2020.Here we introduce a known Drosophila melanogaster temperature sensitive embryonic lethal mutation into Bactrocera tryoni, a serious horticultural pest in Australia. A non-synonymous point mutation in the D. melanogaster gene shibire causes embryonic lethality at 29 degrees C and ... Keywords: Bactrocera dorsalis, Drosophia, genetic biocontrol, genetic sexing strain, SIT, sterile insect technique, temperature sensitivity |
Contact
David O’Brochta
Foundation for the
National Institutes of Health
geneconvenevi@fnih.org
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