Keywords: temperature sensitivity

Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata

Sollazzo, G., Nikolouli, K., Gouvi, G. et al.,  BMC Biotechnology,  24. 2024.
The Mediterranean fruit fly, Ceratitis capitata, is a significant agricultural pest managed through area-wide integrated pest management (AW-IPM) including a sterile insect technique (SIT) component. Male-only releases increase the efficiency and cost-effectiveness of SIT ...
Keywords: , , , , , ,

Next-generation CRISPR gene-drive systems using Cas12a nuclease

S. Sanz Juste, E. M. Okamoto, X. Feng and V. L. Del Amo,  bioRxiv,  2023.02.20.529271. 2023.
One method for reducing the impact of vector-borne diseases is through the use of CRISPR-based gene drives, which manipulate insect populations due to their ability to rapidly propagate desired genetic traits into a target population. However, all current gene drives employ a ...
Keywords: , , , , , ,

Next-generation CRISPR gene-drive systems using Cas12a nuclease

S. Sanz Juste, E. M. Okamoto, X. Feng and V. L. Del Amo,  bioRxiv,  2023.02.20.529271. 2023.
One method for reducing the impact of vector-borne diseases is through the use of CRISPR-based gene drives, which manipulate insect populations due to their ability to rapidly propagate desired genetic traits into a target population. However, all current gene drives employ a ...
Keywords: , , , , , ,

Precise single base substitution in the shibire gene by CRISPR/Cas9-mediated homology directed repair in Bactrocera tryoni

A. Choo, E. Fung, I. Y. Chen, R. Saint, P. Crisp and S. W. Baxter,  BMC Genetics,  21. 2020.
Here we introduce a known Drosophila melanogaster temperature sensitive embryonic lethal mutation into Bactrocera tryoni, a serious horticultural pest in Australia. A non-synonymous point mutation in the D. melanogaster gene shibire causes embryonic lethality at 29 degrees C and ...
Keywords: , , , , , ,