Keywords: transformation
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CRISPR-mediated knock-in of transgenes into the malaria vector Anopheles funestusC. Quinn, A. Anthousi, C. Wondji and T. Nolan, G3 Genes Genomes Genetics, 11. 2021.
The ability to introduce mutations, or transgenes, of choice to precise genomic locations has revolutionized our ability to understand how genes and organisms work. In many mosquito species that are vectors of various human diseases, the advent of CRISPR genome editing tools has ... |
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CRISPR-mediated knock-in of transgenes into the malaria vector Anopheles funestusC. Quinn, A. Anthousi, C. Wondji and T. Nolan, bioRxiv, 2021.03.31.437891. 2021.
We describe herein an optimised transformation system based on the germline delivery of CRISPR components that allows efficient cleavage of a previously validated genomic site and preferential repair of these cut sites via homology-directed repair (HDR), which allows introduction ... |
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The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in Transgenic Aedes aegyptiA. E. Williams, I. Sanchez-Vargas, W. R. Reid, J. Y. Lin, A. W. E. Franz and K. E. Olson, Viruses, 12:18. 2020.
We used CRISPR/Cas9 to re-target a previously characterized locus (Chr2:321382225) and engineered mosquitoes expressing an inverted repeat (IR) dsRNA against the NS3/4A region of the ZIKV genome. Small RNA analysis revealed that the IR effector triggered the mosquito's siRNA ... |
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Mariner transposition and transformation of the yellow fever mosquito, Aedes aegyptiC. J. Coates, N. Jasinskiene, L. Miyashiro and A. A. James, Proceedings of the National Academy of Sciences of the United States of America, 95:3748-3751. 1998.
The mariner transposable element is capable of interplasmid transposition in the embryonic soma of the yellow fever mosquito, Aedes aegypti. To determine if this demonstrated mobility could be utilized to genetically transform the mosquito, a modified mariner element marked with ... |
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Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the houseflyN. Jasinskiene, C. J. Coates, M. Q. Benedict, A. J. Cornel, C. S. Rafferty, A. A. James and F. H. Collins, Proceedings of the National Academy of Sciences of the United States of America, 95:3743-3747. 1998.
The mosquito Aedes aegypti is the world's most important vector of yellow fever and dengue viruses, Work is currently in progress to control the transmission of these viruses by genetically altering the capacity of wild Ae, aegypti populations to support virus replication. The ... |
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Genetic-transformation of Drosophila with transposable element vectorsG. M. Rubin and A. C. Spradling, Science, 218:348-353. 1982.
Exogenous DNA sequences were introduced into the Drosophila germ line. A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected ... |
