Keywords: transgenic
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DIPA-CRISPR gene editing in the yellow fever mosquito <em>Aedes aegypti</em> (Diptera: Culicidae)S. Yu, T. Momoyo, O. Manabu, K. Hirotaka and D. Takaaki, bioRxiv, 2023.04.07.535996. 2023.
Current methods for gene editing in insects rely on embryonic microinjection, which can be challenging for non-specialist laboratories. Recently, an alternative method known as direct parental CRISPR (DIPA-CRISPR) was developed. This method involves injecting commercial Cas9 ... |
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Combining transgenesis with paratransgenesis to fight malariaW. Huang, J. Vega-Rodriguez, C. Kizito, S.-J. Cha and M. Jacobs-Lorena, eLife, 11:e77584. 2022.
Malaria is among the deadliest infectious diseases, and Plasmodium, the causative agent, needs to complete a complex development cycle in its vector mosquito for transmission to occur. Two promising strategies to curb transmission are transgenesis, consisting of genetically ... |
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Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectorsN. A. Ratcliffe, J. P. Furtado Pacheco, P. Dyson, H. C. Castro, M. S. Gonzalez, P. Azambuja and C. B. Mello, Parasites and Vectors, 15:112. 2022.
This article presents an overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors. It first briefly summarises some of the disease-causing pathogens vectored by insects and emphasises the need for innovative control methods to counter the ... |
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First ever gene-edited ticks offer new weapons against Lyme diseaseN. Lavars, New Atlas, 2022.
Gene editing in ticks had been thought to be impossible until now, and with good reason. Tick embryos are very tricky to inject because the egg that contains them has a tough layer on the outside, high pressure levels inside, and is also coated in a waxy layer the mothers create ... |
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Genetically Modified Mosquitoes — What’s The Real Story?SPW Staff, Southeast Product Weekly, 2021.
You’ve heard about the genetically modified mutant mosquitoes being released in the Florida Keys — but what exactly is going on, and how, and why? Basically, researchers in parts of the Florida Keys are releasing male mosquitoes that have been genetically modified to produce ... |
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Mosquito transgenesis for malaria controlS. Dong, Y. Dong, M. L. Simões and G. Dimopoulos, Trends in Parasitology, 2021.
Malaria is one of the deadliest diseases. Because of the ineffectiveness of current malaria-control methods, several novel mosquito vector-based control strategies have been proposed to supplement existing control strategies. Mosquito transgenesis and gene drive have emerged as ... |
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UF/IFAS Researchers Explain Science Behind Genetically Modified MosquitoesPCT Staff, Pest Control Technology, 2021.
South Florida residents seeking science-based information about genetically modified mosquitoes can access a new, online resource from University of Florida scientists at the UF/IFAS Florida Medical Entomology Laboratory.“ Genetically Modified Mosquitoes” is the latest ... |
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Cas9-Mediated Gene-Editing in the Black-Legged Tick, Ixodes Scapularis, by Embryo Injection and ReMOT Control.A. a. P. Sharma, Michael N. and Reyes, Jeremiah B. and Chana, Randeep and Yim, Won C. and Heu, Chan C. and Kim, Donghun and Chaverra-Rodriguez, Duverney and Rasgon, Jason L. and Harrell, Robert A. and Nuss, Andrew B. and Gulia-Nuss, Monika,, Cell Reports, 2021.
Despite their capacity to acquire and pass on an array of debilitating pathogens, research on ticks has lagged behind other arthropod vectors, such as mosquitoes, largely because of a lack of genetic and molecular tools. CRISPR-Cas9 is transforming non-model organism research; ... |
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Genetically Modified MosquitoesE. P. Caragata, Y. Lee and E. A. Buckner, UF IFAS Extension Service, 2021.
Genetically modified (GM) mosquitoes are controversial, partly because of misinformation. This publication provides science-based information about GM mosquitoes to the public and anyone involved in mosquito control. It explains what GM mosquitoes are and why they are being ... |
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CRISPR-mediated knock-in of transgenes into the malaria vector Anopheles funestusC. Quinn, A. Anthousi, C. Wondji and T. Nolan, G3 Genes Genomes Genetics, 11. 2021.
The ability to introduce mutations, or transgenes, of choice to precise genomic locations has revolutionized our ability to understand how genes and organisms work. In many mosquito species that are vectors of various human diseases, the advent of CRISPR genome editing tools has ... |
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New CRISPR Tools Can Help Contain Mosquito Disease TransmissionAnonymous, labcompare, 2021.
Scientists have now developed several genetic editing tools that help pave the way to an eventual gene drive designed to stop Culex mosquitoes from spreading disease. As detailed in the journal Nature Communications, Xuechun Feng, Valentino Gantz and their colleagues at Harvard ... |
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Researchers Create New CRISPR Tools to Help Contain Mosquito Disease TransmissionM. Aguilera, UC San Diego News Center, 2021.
Much less genetic engineering has been devoted to Culex genus mosquitoes, which spread devastating afflictions stemming from West Nile virus—the leading cause of mosquito-borne disease in the continental United States—as well as other viruses such as the Japanese encephalitis ... |
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New CRISPR tools help contain mosquito disease transmission: Genetics toolkit targets less researched Culex mosquitoes, which transmit West Nile virus and avian malaria.University of California - San Diego, ScienceDaily, 2021.
Since the onset of the CRISPR genetic editing revolution, scientists have been working to leverage the technology in the development of gene drives that target pathogen-spreading mosquitoes such as Anopheles and Aedes species, which spread malaria, dengue and other ... |
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Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoesX. Feng, V. López Del Amo, E. Mameli, M. Lee, A. L. Bishop, N. Perrimon and V. M. Gantz, Nature Communications, 12:2960. 2021.
Culex mosquitoes are a global vector for multiple human and animal diseases, including West Nile virus, lymphatic filariasis, and avian malaria, posing a constant threat to public health, livestock, companion animals, and endangered birds. While rising insecticide resistance has ... |
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Genetic Manipulation of Ticks: A Paradigm Shift in Tick and Tick-Borne Diseases ResearchA. Nuss, A. Sharma and M. Gulia-Nuss, Frontiers in Cellular and Infection Microbiology, 11:7. 2021.
Ticks are obligate hematophagous arthropods that are distributed worldwide and are one of the most important vectors of pathogens affecting humans and animals. Despite the growing burden of tick-borne diseases, research on ticks has lagged behind other arthropod vectors, such as ... |
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Introduction of a cold sensitivity-conferring mutation into the RTA-Bddsx hybrid system of Bactrocera dorsalis for establishment of a thermally controllable homozygous lineS. M. Dai, C. Y. Huang and C. Chang, Pest Management Science, 7. 2021.
BACKGROUND For efficient control of the economically important fruit pest Bactrocera dorsalis, a hybrid system combining ricin toxicity and sex-related alternative splicing of the doublesex gene has been developed. This system exhibits the expected female-specific lethal effect; ... |
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CRISPR-mediated knock-in of transgenes into the malaria vector Anopheles funestusC. Quinn, A. Anthousi, C. Wondji and T. Nolan, bioRxiv, 2021.03.31.437891. 2021.
We describe herein an optimised transformation system based on the germline delivery of CRISPR components that allows efficient cleavage of a previously validated genomic site and preferential repair of these cut sites via homology-directed repair (HDR), which allows introduction ... |
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Genetically Encoded CRISPR components Yield Efficient Gene Editing in the Invasive Pest, Drosophila suzukiiN. P. Kandul, E. J. Belikoff, J. Liu, A. Buchman, F. Li, A. Yamamoto, T. Yang, I. Shriner, M. J. Scott and O. Akbari, bioRxiv, 2021.03.15.435483. 2021.
Here we have developed transgenic strains that encode three different terminators and four different promoters to express Cas9 in both the soma and/or germline of SWD. The Cas9 lines were evaluated through genetic crossing to transgenic lines that encode single guide RNAs ... |
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Genetically modified mosquitoes for better healthD. Devis, COSMOS, 2021.
One method of preventing these mosquito-born diseases is to use insecticides to kill the mozzies and remove them, but sometimes this only works as a short term solution, or has unintended devasting effects on the ecosystem. Another method for decreasing the number of ... |
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Quantifying the risk of vector-borne disease transmission attributable to genetically modified vectorsG. R. Hosack, A. Ickowicz and K. R. Hayes, Royal Society Open Science, 8:201525. 2021.
The relative risk of disease transmission caused by the potential release of transgenic vectors, such as through sterile insect technique or gene drive systems, is assessed with comparison with wild-type vectors. The probabilistic risk framework is demonstrated with an assessment ... |
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Optimized CRISPR tools and site-directed transgenesis in Culex quinquefasciatus mosquitoes for gene drive developmentX. Feng, V. Lopez Del Amo, E. Mameli, M. Lee, A. L. Bishop, N. Perrimon and V. M. Gantz, bioRxiv, 2021.02.10.430702. 2021.
Here, we developed a Culex-specific Cas9/gRNA expression toolkit and used site-directed homology-based transgenesis to generate and validate a Culex quinquefasciatus Cas9-expressing line. We showed that gRNA scaffold variants improve transgenesis efficiency in both Culex and ... |
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Mosquitoes genetically modified to be resistant to ZikaStaff, Lab+Life Scientist, 2021.
Researchers have wrestled with different strategies for controlling the spread of Zika virus, which is transmitted to humans from female mosquito bites. One approach, which has been approved by the US Environmental Protection Agency, will see more than 750 million genetically ... |
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Genetically-modified mosquitoes key to stopping Zika virus spreadUniversity of Missouri, Medical Xpress, 2021.
Alexander Franz, an associate professor in the MU College of Veterinary Medicine, collaborated with researchers at Colorado State University by using CRISPR gene-editing technology to produce mosquitoes that are unable to replicate Zika virus and therefore cannot infect a human ... |
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Genetically Engineered Fish: Potential Impacts on Aquaculture, Biodiversity, and the EnvironmentR. A. Dunham and B. Su, GMOs: Implications for Biodiversity Conservation and Ecological Processes, 2020.
Studies on transgenic fish for the aquaculture industry have focused on improving growth rates, enhancing disease resistance, altering body composition, acting as biological factories for medical proteins, and even altering temperature tolerance and coloration. The future impact ... |
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Mosquito transgenics and courtship songsH. Hurd, BugBitten BMC, 2020.
As a female flies into the swarm she is detected by the sound of her wing beat and males identify females by a phonotactic response to the specific sound frequency produced by her wing beat. A male and female will then attune to each other by altering the frequency of their ... |
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Genetic engineering and bacterial pathogenesis against the vectorial capacity of mosquitoesM. Qasim, H. M. Xiao, K. He, M. A. A. Omar, F. L. Liu, S. Ahmed and F. Li, Microbial Pathogenesis, 147:8. 2020.
Here we aimed to focus on the role of bacterial pathogenesis and molecular tactics for the management of mosquitoes and their vectorial capacity. |
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How do you make a gene drive mosquito?GeneConvene Virtual Institute, GeneConvene Global Collaborative, 2020.
This short video explains and illustrates how transgenic mosquitoes are made in the laboratory. While mosquitoes are the focus of the video, the process shown is used to create transgenic insects of almost any species. |
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Auditing preparedness for vector control field studiesC. M. Collins and M. M. Quinlan, American Journal of Tropical Medicine and Hygiene, 102:707-710. 2020.
The value of baseline entomological data to any future area-wide release campaign relies on the application of consistent methods to produce results comparable across different times and places in a stepwise progression to larger releases. Traditionally, standard operating ... |
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A potential new tool for the toolbox: assessing gene drives for eradicating invasive rodent populationsK. J. Campbell, J. R. Saah, P. R. Brown, J. Godwin, F. Gould, G. R. Howald, A. Piaggio, P. Thomas, D. M. Tompkins, D. Threadgill, J. Delborne, D. Kanavy, T. Kuiken, H. Packard, M. Serr and A. Shiels, Island invasives: scaling up to meet the challenge, 2019.
Invasive rodents have significant negative impacts on island biodiversity. All but the smallest of rodent eradications currently rely on island-wide rodenticide applications. Although signifi cant advances have been made in mitigating unintended impacts, rodent eradication on ... |
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Malaria Control with Genetically Manipulated Insect VectorsL. Alphey, C. B. Beard, P. Billingsley, M. Coetzee, A. Crisanti, C. Curtis, P. Eggleston, C. Godfray, J. Hemingway, M. Jacobs-Lorena, A. A. James, F. C. Kafatos, L. G. Mukwaya, M. Paton, J. R. Powell, W. Schneider, T. W. Scott, B. Sina, R. Sinden, S. Sink, Science, 298:119. 2002.
At a recent workshop, experts discussed the benefits, risks, and research priorities associated with using genetically manipulated insects in the control of vector-borne diseases. |
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Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the houseflyN. Jasinskiene, C. J. Coates, M. Q. Benedict, A. J. Cornel, C. S. Rafferty, A. A. James and F. H. Collins, Proceedings of the National Academy of Sciences of the United States of America, 95:3743-3747. 1998.
The mosquito Aedes aegypti is the world's most important vector of yellow fever and dengue viruses, Work is currently in progress to control the transmission of these viruses by genetically altering the capacity of wild Ae, aegypti populations to support virus replication. The ... |
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Gene transfer into the Medfly, Ceratitis capitata, using a Drosophila hydei transposable element.T. G. Loukeris, I. Livadaras, B. Arca, S. Zabalou and C. Savakis, Science, 270:2002-2005. 1995.
Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly (medfly) Ceratitis capitata with a germline transformation system based on the transposable element Minos from Drosophila hydei. Transformants were identified as phenotypic ... |
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Genetic-transformation of Drosophila with transposable element vectorsG. M. Rubin and A. C. Spradling, Science, 218:348-353. 1982.
Exogenous DNA sequences were introduced into the Drosophila germ line. A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected ... |
