Keywords: vertebrates

Progress Toward Zygotic and Germline Gene Drives in Mice

C. Pfitzner, M. A. White, S. G. Piltz, M. Scherer, F. Adikusuma, J. N. Hughes and P. Q. Thomas,  The CRISPR Journal,  3:388-397. 2020.
Here, we investigated the efficiency of CRISPR-Cas9-based gene drives in Mus musculus by constructing "split drive" systems where gRNA expression occurs on a separate chromosome to Cas9, which is under the control of either a zygotic (CAG) or germline (Vasa) promoter.
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Development of zygotic and germline gene drives in mice

C. Pfitzner, J. N. Hughes, M. A. White, M. Scherer, S. G. Piltz and P. Q. Thomas,  bioRxiv,  2020.
Here we investigated the efficiency of CRISPR/Cas9-based gene drives in Mus musculus by constructing "split drive" systems with Cas9 under the control of zygotic (CAG) or germline (Vasa) promoters.
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RNAi: Applications in Vertebrate Pest Management

K. E. Horak,  Trends in Biotechnology,  38:1200-1202. 2020.
the development of novel control technologies must be focused on species specificity and low environmental impact. Sequence-specific gene silencing via RNAi holds promise for effective management of pest wildlife.
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