Keywords: virus

Differential viral RNA methylation contributes to pathogen blocking in Wolbachia-colonized arthropods

T. Bhattacharya, L. Yan, J. M. Crawford, H. Zaher, I. L. G. Newton and R. W. Hardy,  PLoS Pathogens,  18:e1010393. 2022.
Arthropod endosymbiont Wolbachia pipientis is part of a global biocontrol strategy to reduce the replication of mosquito-borne RNA viruses such as alphaviruses. We previously demonstrated the importance of a host cytosine methyltransferase, DNMT2, in Drosophila and viral RNA as a ...
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Wolbachia reduces virus infection in a natural population of Drosophila

R. Cogni, S. D. Ding, A. C. Pimentel, J. P. Day and F. M. Jiggins,  Communications Biology,  4:1327. 2021.
Wolbachia is a maternally transmitted bacterial symbiont that is estimated to infect approximately half of arthropod species. In the laboratory it can increase the resistance of insects to viral infection, but its effect on viruses in nature is unknown. Here we report that in a ...
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Wolbachia-Conferred Antiviral Protection Is Determined by Developmental Temperature

E. Chrostek, N. Martins, M. S. Marialva and L. Teixeira,  mBio,  e0292320. 2021.
Overall, we show that Wolbachia-conferred antiviral protection is temperature dependent, being present or absent depending on the environmental conditions. This interaction likely impacts Wolbachia-host interactions in nature and, as a result, frequencies of host and symbionts in ...
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Genetically Modifying Bats Could Prevent the Next Pandemic, Scientists Say

G. Dutton,  BioSpace,  2021.
The next COVID pandemic could be prevented by using a gene drive to preemptively edit the genome of bats to prevent them from becoming hosts for coronaviruses, according to a proposal by scientists from Israel’s Interdisciplinary Center (IDC) Herzelia and the National ...
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Targeting Conserved Sequences Circumvents the Evolution of Resistance in a Viral Gene Drive against Human Cytomegalovirus

M. Walter, R. Perrone, E. Verdin and F. Goodrum,  Journal of Virology,  95:e00802-21. 2021.
Gene drives are genetic systems designed to efficiently spread a modification through a population. They have been designed almost exclusively in eukaryotic species, especially in insects. We recently developed a CRISPR-based gene drive system in herpesviruses that relies on ...
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Targeting conserved sequences circumvents the evolution of resistance in a viral gene drive against human cytomegalovirus

M. Walter, R. Perrone and E. Verdin,  Journal of virology,  2021.
Here, we analyze in cell culture experiments the evolution of resistance in a viral gene drive against human cytomegalovirus. We report that, after an initial invasion of the wildtype population, a drive-resistant population is positively selected over time and outcompetes gene ...
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Targeting evolutionary conserved sequences circumvents the evolution of resistance in a viral gene drive against human cytomegalovirus

M. Walter, R. Perrone and E. Verdin,  bioRxiv,  2021.01.08.425902. 2021.
Here, we analyze in cell culture experiments the evolution of resistance in a gene drive against human cytomegalovirus. We report that after an initial invasion of the wildtype population, a drive-resistant population is positively selected over time and outcompetes gene drive ...
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Wolbachia strain wAlbB maintains high density and dengue inhibition following introduction into a field population of Aedes aegypti

N. A. Ahmad, M.-V. Mancini, T. H. Ant, J. Martinez, G. M. R. Kamarul, W. A. Nazni, A. A. Hoffmann and S. P. Sinkins,  Philosophical Transactions of the Royal Society B: Biological Sciences,  376:20190809. 2020.
Here, wAlbB-carrying Ae. aegypti collected from the field 20 months after the cessation of releases showed no reduction in Wolbachia density or tissue distribution changes compared to a wAlbB laboratory colony. The wAlbB strain continued to induce complete unidirectional ...
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Gene Drives Could Kill Mosquitoes And Suppress Herpesvirus Infections

A. Berezow,  American Council on Science and Health,  2020.
A team of researchers writing in the journal Nature Communications has shown that a gene drive can be used to suppress infection with cytomegalovirus, a type of herpesvirus.
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Viral gene drive in herpesviruses

M. Walter and E. Verdin,  Nature Communications,  11:4884. 2020.
Here, we report on a gene drive system that allows the spread of an engineered trait in populations of DNA viruses and, in particular, herpesviruses.
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Viral gene drive in herpesviruses

Walter, M. and E. Verdin,  bioRxiv,  2019:717017. 2019.
Herpesviruses are ubiquitous pathogens in need of novel therapeutic solutions. Current engineered gene drive strategies rely on sexual reproduction, and are thought to be restricted to sexual organisms. Here, we report on the design of a novel gene drive system that allows the ...
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Variability in the durability of CRISPR-Cas immunity

Chabas, HN, A.; Meaden, S.; Westra, E. R.; Tremblay, D. M.; Pradier, L.; Lion, S.; Moineau, S.; Gandon, S.,  Philosophical Transactions of the Royal Society B-Biological Sciences,  374:1-9. 2019.
The durability of host resistance is challenged by the ability of pathogens to escape the defence of their hosts. Understanding the variability in the durability of host resistance is of paramount importance for designing more effective control strategies against infectious ...
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