Keywords: X-shredder

The haplolethal gene wupA of Drosophila exhibits potential as a target for an X-poisoning gene drive

Lawler, Clancy D ,Parra Nuñez, Ana Karla ,Hernandes, Natalia ,Bhide, Soumitra ,Lohrey, Isabelle ,Baxter, Simon ,Robin, Charles,  G3 Genes Genome Genetics,  jkae025. 2024.
We examine this ‘X-poisoning’ strategy by targeting four of the 11 known X-linked haplolethal/haplosterile genes of Drosophila melanogaster with CRISPR/Cas9. We find that targeting the wupA gene during spermatogenesis skews the sex ratio so fewer than 14% of progeny are ...
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The haplolethal gene wupA of Drosophila exhibits potential as a target for an X-poisoning gene drive

Clancy D. Lawler, Ana Karla Parra Nuñez, Natalia Hernandes, Soumitra Bhide, Isabelle Lohrey, Simon Baxter, Charles Robin,  bioRxiv,  2024.
A synthetic gene drive that targets haplolethal genes on the X-chromosome can skew the sex ratio towards males. Like an ‘X-shredder’ it does not involve ‘homing’ and that has advantages including the reduction of gene drive resistance allele formation. We examine this ...
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The haplolethal gene wupA of Drosophila exhibits potential as a target for an X-poisoning gene drive

Lawler C., D. , Hernandes, N., Nunez, A. K. P., Bhide, S., Baxter, S. and Robin, C.,  bioRxiv,  2023.06.23.546292. 2023.
A synthetic gene drive that targets haplolethal genes on the X-chromosome can skew the sex ratio towards males. Like an X-shredder strategy it does not involve homing and that has advantages including the reduction in gene drive resistance allele formation. We examine this ...
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Introgression of a synthetic sex ratio distortion transgene into different genetic backgrounds of Anopheles coluzzii

P. Pollegioni, T. Persampieri, R. L. Minuz, A. Bucci, A. Trusso, S. Di Martino, C. Leo, M. Bruttini, M. Ciolfi, A. M. Waldvogel, F. Tripet, A. Simoni, A. Crisanti and R. Müller,  Insect Molecular Biology,  2022.
The development of genetically modified mosquitoes (GMM) and their subsequent field release offers innovative approaches for vector control of malaria. A non-gene drive self-limiting male-bias Ag(PMB)1 strain has been developed in a 47-year-old laboratory G3 strain of Anopheles ...
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Anopheles homing suppression drive candidates exhibit unexpected performance differences in simulations with spatial structure

S. E. Champer, I. K. Kim, A. G. Clark, P. W. Messer and J. Champer,  eLife,  11:e79121. 2022.
Recent experiments have produced several Anopheles gambiae homing gene drives that disrupt female fertility genes, thereby eventually inducing population collapse. Such drives may be highly effective tools to combat malaria. One such homing drive, based on the zpg promoter ...
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Slow and steady wins the race: spatial and stochastic processes and the failure of suppression gene drives

J. F. Paril and B. L. Phillips,  Molecular Ecology,  2022.
Gene drives that skew sex ratios offer a new management tool to suppress or eradicate pest populations. Early models and empirical work suggest that these suppression drives can completely eradicate well-mixed populations, but models that incorporate stochasticity and space ...
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Selective targeting of biting females to control mosquito-borne infectious diseases

B. B. Kojin, A. Compton, Z. N. Adelman and Z. Tu,  Trends in Parasitology,  2022.
Mosquitoes are vectors for a number of infectious diseases. Only females feed on blood to provision for their embryos and, in doing so, transmit pathogens to the associated vertebrate hosts. Therefore, sex is an important phenotype in the context of genetic control programs, both ...
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Finding the strongest gene drive: Simulations reveal unexpected performance differences between Anopheles homing suppression drive candidates

S. E. Champer, I. K. Kim, A. G. Clark, P. W. Messer and J. Champer,  bioRxiv,  2022.03.28.486009. 2022.
Recent experiments have produced several Anopheles gambiae homing gene drives that disrupt female fertility genes, thereby eventually inducing population collapse. Such drives may be highly effective tools to combat malaria. One such homing drive, based on the zpg promoter ...
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Modeling impact and cost-effectiveness of gene drives for malaria elimination in the Democratic Republic of the Congo

N. Metchanun, C. Borgemeister, G. Amzati, J. von Braun, M. Nikolov, P. Selvaraj and J. Gerardin,  medRxiv,  2020.06.29.20142760. 2021.
Using a spatially explicit, agent-based model of malaria transmission in eight representative provinces of the Democratic Republic of the Congo, we predict the impact and cost-effectiveness of integrating driving-Y gene drive mosquitoes in malaria elimination strategies that ...
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The New Yorker Magazine: Gene Drives as a Tool for Saving Nature

E. Heber,  Island Conservation,  2021.
In a recent New Yorker Magazine article, entitled “CRISPR and the Splice to Survive,” journalist and best-selling author Elizabeth Kolbert dives into the world of gene drive research. She touches on aspects of gene drive research from altering the toxin produced by cane toads ...
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Role of gene drives in malaria elimination strategy: modeling impact and cost-effectiveness in the Democratic Republic of the Congo

N. Metchanun, C. Borgemeister, J. von Braun, M. Nikolov, P. Selvaraj and J. Gerardin,  medRxiv,  2020.
The tremendous burden of malaria has led to renewed efforts on malaria elimination and the development of novel tools for application where existing tools fall short. Gene drive mosquitoes, where transgenes and their associated phenotypes are efficiently propagated to future ...
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Genetic Biocontrol – An Overview

GeneConvene Global Collaborative,,  GeneConvene Global Collaborative,  2020.
This video explains what genetic biocontrol is and surveys various technologies that can be consider genetic biocontrol technologies.  It offers a conceptual organization of the various technologies based on the potential of genetic biocontrol organisms to persist and spread in ...
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