Keywords: yellow-fever mosquito
The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in Transgenic Aedes aegyptiA. E. Williams, I. Sanchez-Vargas, W. R. Reid, J. Y. Lin, A. W. E. Franz and K. E. Olson, Viruses, 12:18. 2020.
We used CRISPR/Cas9 to re-target a previously characterized locus (Chr2:321382225) and engineered mosquitoes expressing an inverted repeat (IR) dsRNA against the NS3/4A region of the ZIKV genome. Small RNA analysis revealed that the IR effector triggered the mosquito's siRNA ...
Keywords: Aedes aegypti, antiviral, arbovirus, Cas9, CRISPR, Gene drive, immunity, RNAi, siRNAs, transformation, transgenesis, vector, yellow-fever mosquito, Zika virus
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National Institutes of Health