Multiplexing gRNAs to Hedge Against Resistance to Gene Drive
Multiplexing gRNAs to Hedge Against Resistance to Gene Drive
4720Travis Van Warmerdam, IGTRCN, 2018-11-25 00:00:00.
Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for viability or female fertility in Drosophila melanogaster.
