Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for viability or female fertility in Drosophila melanogaster.
https://www.geneconvenevi.org/wp-content/uploads/2018/09/IGTRCN.png300300Academic Web Pages/wp-content/uploads/2019/10/GC-color-logo-for-header-3277-x-827-1030x260.pngAcademic Web Pages2018-11-25 00:00:002020-04-28 14:25:19Multiplexing gRNAs to Hedge Against Resistance to Gene Drive