Multiplexing gRNAs to Hedge Against Resistance to Gene Drive
Multiplexing gRNAs to Hedge Against Resistance to Gene Drive
Tags: CRISPR, Fruit fly, Replicator/site directed nucleaseTravis Van Warmerdam, IGTRCN, 2018.
Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for viability or female fertility in Drosophila melanogaster.
