Multiplexing gRNAs to Hedge Against Resistance to Gene Drive

Travis Van Warmerdam,  IGTRCN,  2018.

Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for viability or female fertility in Drosophila melanogaster.