A Y chromosome-linked genome editor for efficient population suppression in the malaria vector Anopheles gambiae
A Y chromosome-linked genome editor for efficient population suppression in the malaria vector Anopheles gambiae
Tags: Anopheles, Malaria, Synthetic biology, Vector controlIgnacio Tolosana, Katie Willis, Austin Burt, Matthew Gribble, Tony Nolan, Andrea Crisanti, Federica Bernardini, bioRxiv, 2024.
Genetic control – the deliberate introduction of genetic traits to control a pest or vector population – offers a powerful tool to augment conventional mosquito control tools that have been successful in reducing malaria burden but that are compromised by a range of operational challenges. Self-sustaining genetic control strategies have shown great potential in laboratory settings but hesitancy due to their invasive and persistent nature may delay their implementation. Here instead we describe a self-limiting strategy, designed to have geographically and/or temporally restricted effect, based on a Y chromosome-linked genome editor (YLE). The YLE comprises a CRISPR-Cas9 construct that is always inherited by males yet generates an autosomal dominant mutation that is transmitted to over 90% of the offspring and results in female-specific sterility. Males are unaffected. To our knowledge, our system represents the first engineering of the Y chromosome to generate a genetic control strain for mosquitoes. Mathematical modelling shows that this YLE technology is up to 8 times more efficient for population suppression than optimal versions of other self-limiting strategies.