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How to make a gene drive mosquito?

2024-10-01 13:52:24.
A short explanation

Driving down malaria transmission with engineered gene drive

Frontiers in Genetics,  2022-06-15 13:40:38.

What is genome editing?

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NHGRI,  NHGRI,  2019-08-15 17:41:15.
Genome editing is a method that lets scientists change the DNA of many organisms, including plants, bacteria, and animals. Editing DNA can lead to changes in physical traits, like eye color, and disease risk. Scientists use different technologies to do this.

A Multiple Gene Drive System

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Ferdinand Nanfack Minkeu,  IGTRCN,  2019-01-14 00:00:00.
Yan & Finnigan, (2018) recently published a paper in Scientific Reports describing an artificial multi-locus gene drive system by using a single Cas9 and three guide RNA (gRNA) in the budding yeast Saccharomyces cerevisiae. Nuclease-based gene drives do not follow the typical Mendel patterns of inheritance and lead quickly to the spread of gene drive elements through a given population.

Multiplexing gRNAs to Hedge Against Resistance to Gene Drive

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Travis Van Warmerdam,  IGTRCN,  2018-11-25 00:00:00.
Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for viability or female fertility in Drosophila melanogaster.

Cas9 Gene Drive, Sex-Conversion and Evolved Resistance

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Anna Buchman,  IGTRCN,  2018-09-24 00:00:00.
In a recent manuscript, KaramiNejadRanjbar et al. demonstrate the development of a proof of principle Cas9-based suppression gene drive in D. melanogaster that can be applied to pest insects, and discuss the implications of resistance allele formation for practical use of such a drive system.

What is CRISPR?

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A. Vidyasagar,  LiveScience,  2018-04-21 17:17:15.
CRISPR technology is a simple yet powerful tool for editing genomes. It allows researchers to easily alter DNA sequences and modify gene function. Its many potential applications include correcting genetic defects, treating and preventing the spread of diseases and improving crops. However, its promise also raises ethical concerns.