Genome engineering in insects: focus on the CRISPR/Cas9 system

Hillary, V. Edwin Ceasar, Stanislaus Antony Ignacimuthu, S.,  Genome Engineering via CRISPR-Cas9 System,  2020.

Genome engineering is a precise tool used to alter the genome of desired organism. Zinc finger nuclease (ZFN), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR), and the CRISPR-associated RNA guided endonuclease Cas9 (CRISPR/Cas9) are the major genome engineering tools used in these days. CRISPR/Cas9 system has redeemed the precise genome engineering in different species including insects. This chapter covers the details on genome engineering studies reported in various insects including mosquitoes, butterflies, silkworm and fruit fly with a focus on CRISPR/Cas9 system. Many studies have been reported on application of ZFN, TALEN and CRISPR/Cas9 in insects. In recent years, many scientists have adopted CRISPR/Cas9 system for insect genome modification due to its affordability and quick designing of the constructs. We also discuss the details and applications of gene drive. Further studies with CRISPR/Cas9in insects will help researchers to find an effective strategy to combat the vector borne diseases spread by insects like mosquitoes.

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