Scholarly Literature
This is an aggregation of scholarly literature from peer-reviewed journals, bioRxiv , medRxiv and arXiv preprint servers focused on natural and engineered selfish genetic elements and genetic biocontrol. New citations are added as they appear. This content is intended for anyone interested in the scholarly literature on gene drive and genetic biocontrol.
Intra-lineage microevolution of Wolbachia leads to the emergence of new cytoplasmic incompatibility patterns
Tags: Arbovirus, Malaria, WolbachiaNamias A, Ngaku A, Makoundou P, Unal S, Sicard M, Weill M, PLoS Biology, 2024.
Mosquitoes of the Culex pipiens complex are worldwide vectors of arbovirus, filarial nematodes, and avian malaria agents. In these hosts, the endosymbiotic bacteria Wolbachia induce cytoplasmic incompatibility (CI), i.e., reduced embryo viability in so-called incompatible ...
Transgenic expression of cif genes from Wolbachia strain wAlbB recapitulates cytoplasmic incompatibility in Aedes aegypti
Tags: Gene editingMcNamara, C.J., Ant, T.H., Harvey-Samuel, T. et al., Nature Communications, 15. 2024.
The endosymbiotic bacteria Wolbachia can invade insect populations by modifying host reproduction through cytoplasmic incompatibility (CI), an effect that results in embryonic lethality when Wolbachia-carrying males mate with Wolbachia-free females. Here we describe a transgenic ...
Transforming malaria prevention and control: the prospects and challenges of gene drive technology for mosquito management
Tags: CRISPR, Gene driveYusuf Amuda Tajudeen, Habeebullah Jayeola Oladipo, Iyiola Olatunji Oladunjoye, Muhammad Kamaldeen Oladipo, Hameedat Damilola Shittu, Imam-Fulani Abdulmumeen, Abdullateef Opeyemi Afolabi and Mona Said El-Sherbini, Annals of Medicine, 55. 2024.
In the era of insecticides and anti-malarial drug resistance, gene drive technology holds considerable promise for malaria control. Gene drive technology deploys genetic modifications into mosquito populations to impede their ability to transmit the malaria parasite. This can be ...
A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations
Tags: Aedes, CRISPR, Gene editing, Synthetic biologyAnderson, M.A.E., Gonzalez, E., Edgington, M.P. et al., Nature Communications, 15. 2024.
Aedes aegypti is the main vector of several major pathogens including dengue, Zika and chikungunya viruses. Classical mosquito control strategies utilizing insecticides are threatened by rising resistance. This has stimulated interest in new genetic systems such as gene ...
Evidence of Differences in Cellular Regulation of Wolbachia-Mediated Viral Inhibition between Alphaviruses and Flaviviruses
Tags: WolbachiaRainey SM, Lefteri DA, Darby C, Kohl A, Merits A, Sinkins SP., Viruses, 16(1):115. 2024.
The intracellular bacterium Wolbachia is increasingly being utilised in control programs to limit the spread of arboviruses by Aedes mosquitoes. Achieving a better understanding of how Wolbachia strains can reduce viral replication/spread could be important for the long-term ...
Stakeholder engagement in the development of genetically modified mosquitoes for malaria control in West Africa: lessons learned from 10 years of Target Malaria’s work in Mali
Tags: Stakeholder engagement, Vector controlBakara Dicko, Souleymane Kodio, Hatouma Samoura, Fatoumata Traoré, Naima Sykes, Mouhamed Drabo, Delphine Thizy, Isabelle Coche, Benjamin Robinson, Kadiatou Sanogo, Bilkissou Yagouré, Samba Diop, Mamadou B. Coulibaly, Frontiers in Bioengineering and Biotechnology, 11. 2024.
From 2012 to 2023, the Malaria Research and Training Center (MRTC), based out of the University of Sciences, Techniques and Technologies of Bamako (USTTB), was part of the Target Malaria research consortium working towards developing novel gene drive-based tools for controlling ...
The haplolethal gene wupA of Drosophila exhibits potential as a target for an X-poisoning gene drive
Tags: Fruit fly, Gene drive, Gene drive synthetic, Population suppression, Sex distorter, X chromosomeClancy D. Lawler, Ana Karla Parra Nuñez, Natalia Hernandes, Soumitra Bhide, Isabelle Lohrey, Simon Baxter, Charles Robin, bioRxiv, 2024.
A synthetic gene drive that targets haplolethal genes on the X-chromosome can skew the sex ratio towards males. Like an ‘X-shredder’ it does not involve ‘homing’ and that has advantages including the reduction of gene drive resistance allele formation. We examine this ...
A bumpy road ahead for genetic biocontainment
Tags: Policy, Synthetic biologyGeorge, D.R., Danciu, M., Davenport, P.W. et al, Nature Communications, 2024.
The environmental release of bioengineered organisms is increasingly being suggested for a variety of applications, including bioremediation, biosequestration, bio-mining, environmental biosensing and conservation. The objectives of many environmental release applications shift ...
The epidemiology of imported and locally-acquired dengue in Australia, 2012–2022
Tags: Dengue, Mosquitoes, Oceania, WolbachiaAsma Sohail, Katherine L Anders, Sarah L McGuinness, Karin Leder, Journal of Travel Medicine, 2024.
Dengue is the most important arboviral disease globally, and poses ongoing challenges for control including in non-endemic countries with competent mosquito vectors at risk of local transmission through imported cases. We examined recent epidemiological trends in imported and ...
Recombinant venom proteins in insect seminal fluid reduces female lifespan
Tags: Genetic biocontrol, ResistanceSamuel J. Beach, Maciej Maselko, bioRxiv, 2024.
The emergence of insecticide resistance has increased the need for alternative pest management tools1,2 Numerous genetic biocontrol approaches, which involve the release of genetically modified organisms to control pest populations, are in various stages of development to provide ...
Maxizyme-mediated suppression of chikungunya virus replication and transmission in transgenic Aedes aegypti mosquitoes
Tags: Aedes, Arbovirus, Gene editing, MosquitoesMishra P, Balaraman V, Fraser Jr. M, Frontiers in Microbiology, 14. 2024.
Chikungunya virus (CHIKV) is an emerging mosquito-borne pathogen of significant public health importance. There are currently no prophylactic vaccines or therapeutics available to control CHIKV. One approach to arbovirus control that has been proposed is the replacement of ...
Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, Anastrepha ludens (Diptera: Tephritidae)
Tags: Sterile insect technique (SIT)Ruiz-Montoya L, Sánchez-Rosario M, López-Gómez E, Garcia-Bautista M, Canedo-Texón A, Haymer D, Liedo P, Insects, 15:56. 2024.
The application of the sterile insect technique (SIT) requires the adaptation of insects to mass-rearing conditions. It is generally accepted that this adaptation may include a reduction in genetic diversity and an associated loss of desirable characteristics for the effective ...
Wolbachia still works when it is warm
Tags: Arbovirus, Biological control, Dengue, Ecology, WolbachiaMcKay, A., Nature Ecology and Evolution, 8. 2024.
Wolbachia is a maternally inherited endosymbiotic bacterium that can impede the transmission of viruses such as dengue and Zika by some mosquito vectors to humans. Over the past decade, this self-sustaining disease-control method has been rolled out in cities of increasing size; ...
Gene drive and genetic sex conversion in the global agricultural pest Ceratitis capitata
Tags: CRISPR, Gene drive, Gene drive synthetic, Genetic engineering, Population suppression, Sex distorter, Synthetic biologyMeccariello, A., Hou, S., Davydova, S. et al., Nature Communications, 15:372. 2024.
Homing-based gene drives are recently proposed interventions promising the area-wide, species-specific genetic control of harmful insect populations. Here we characterise a first set of gene drives in a tephritid agricultural pest species, the Mediterranean fruit fly Ceratitis ...
Programmed evolution: Using asexual gene drives to sculpt tumor populations and combat genetic diversity
Tags: Synthetic biologyJustin Pritchard, Scott Leighow, Joshua Reynolds et al., Research Square, 2024.
Resistance evolution is responsible for the failure of most targeted anticancer therapies. Tumor heterogeneity is so profound that pre-existing resistance is thought to be guaranteed at the time that advanced disease is detected. The practice of waiting for treatment failure and ...
Sex or poison? Genetic pest management in the 21st century
Tags: Mosquitoes, Pest management, Sterile insect technique (SIT)Luke Alphey, BMC Biology, 21:289. 2024.
Pests do enormous damage to human and animal health, to agriculture and to biodiversity, with mosquitoes transmitting pathogens, insect larvae eating crops or invasive rodents threatening the last island refuges of endangered birds. This commentary focuses on insects, ...
Limited association between Wolbachia and Plasmodium falciparum infections in natural populations of the major malaria mosquito Anopheles moucheti
Tags: WolbachiaThéo Mouillaud, Audric Berger, Marie Buysse, Nil Rahola, Josquin Daron, Jean-Pierre Agbor, Sandrine N. Sango, Daniel E. Neafsey, Olivier Duron, Diego Ayala, Evolutionary Applications, 16:1999-2006. 2024.
Since the discovery of natural malaria vector populations infected by the endosymbiont bacterium Wolbachia, a renewed interest has arisen for using this bacterium as an alternative for malaria control. Among naturally infected mosquitoes, Anopheles moucheti, a major malaria ...
The mosquito knows no borders: Regional challenges for global confrontation in the dengue battle
Tags: Field trials, Mosquitoes, South/Central AmericaBarçante JMdP, Cherem J, PLoS Neglected Tropical Diseases, 18. 2024.
Dengue fever is a neglected disease with a global impact, and its incidence and geographical reach are rapidly expanding. Global warming marked by higher average temperatures, precipitation, and longer periods of drought could prompt a record number of dengue fever infections ...
Consultation on a draft National Gene Drive Policy Guide
Tags: Gene drive, Oceania, PolicyCommonwealth of Australia (Department of Health and Aged Care), National Gene Technology Scheme, 2024.
The term gene drive is used to describe organisms which have been genetically modified to increase the rate for a particular trait to spread through a sexually reproducing population, spreading the genes or traits through a species at a faster rate than normal inheritance. ...
Editorial overview: Conflicts, conflicts everywhere
Tags: GeneticsHarmit S Malik, Judith E Mank, Current Opinion in Genetics and Development, 83. 2023.
Genetic conflicts are pervasive in biology. They arise when genes, sets of genes, or chromosomes enhance their success at significant cost to their genetic neighbors. In some cases, conflicts occur when a ‘cheater’ subverts the rules of genetic inheritance, pitting the ...
Gene drives, mosquitoes, and ecosystems: An interdisciplinary approach to emerging ethical concerns
Tags: CRISPR, Gene drive, Malaria, MosquitoesRicardo D. Moreno, Luca Valera, Cristián Borgoño, Juan Carlos Castilla, José Luis Riveros, Frontiers in Environmental Science, 11. 2023.
Gene drives are genetic elements that in sexually reproducing organisms spread faster than those transmitted through a Mendelian fashion. Since gene drives can be engineered to modify different aspects of physiology and reproduction, they have been proposed as a new and ...
Antiviral Wolbachia strains associate with Aedes aegypti endoplasmic reticulum membranes and induce lipid droplet formation to restrict dengue virus replication
Tags: Aedes, Arbovirus, Dengue, WolbachiaRobson K. Loterio, Ebony A. Monson, Rachel Templin, Jyotika T. de Bruyne, Heather A. Flores, Jason M. Mackenzie, Georg Ramm, Karla J. Helbig, Cameron P. Simmons, Johanna E. Fraser, Applied and Environmental Microbiology, 2023.
Wolbachia are a genus of insect endosymbiotic bacteria which includes strains wMel and wAlbB that are being utilized as a biocontrol tool to reduce the incidence of Aedes aegypti-transmitted viral diseases like dengue. However, the precise mechanisms underpinning the antiviral ...
Perspectives of African stakeholders on gene drives for malaria control and elimination: a multi-country survey
Tags: AfricaFinda, M.F., Juma, E.O., Kahamba, N.F. et al., Malaria Journal, 22:8384. 2023.
Gene drive modified mosquitoes (GDMMs) have the potential to address Africa’s persistent malaria problem, but are still in early stages of development and testing. Continuous engagement of African stakeholders is crucial for successful evaluation and implementation of these ...
Wolbachia infection negatively impacts Drosophila simulans heat tolerance in a strain- and trait-specific manner
Tags: Fruit fly, WolbachiaLiam F Ferguson, Perran A Ross, Belinda van Heerwaarden, bioRxiv, 2023.
The susceptibility of insects to rising temperatures has largely been measured by their ability to survive thermal extremes. However, until recently, the capacity for maternally inherited endosymbionts to influence insect heat tolerance has been overlooked. Further, the impact of ...
Rapid turnover of pathogen-blocking Wolbachia and their incompatibility loci
Tags: Aedes, Biological control, Evolution, Population modification/replacement, WolbachiaShropshire JD, Conner WR, Vanderpool D, Hoffmann AA, Turelli M, Cooper BS., bioRxiv, 2023.
At least half of all insect species carry maternally inherited Wolbachia alphaproteobacteria, making Wolbachia the most common endosymbionts in nature. Wolbachia spread to high frequencies is often due to cytoplasmic incompatibility (CI), a Wolbachia -induced sperm modification ...
CRISPR/Cas9: a cutting-edge solution for combatting the fall armyworm, Spodoptera frugiperda
Tags: CRISPR, Gene editing, Pest managementGouda, M.N.R., Jeevan, H., Shashank, H.G., Molecular Biology Reports, 51. 2023.
The utilization of CRISPR/Cas9 in Spodoptera frugiperda, commonly known as fall armyworm, presents a groundbreaking avenue for pest management. With its ability to precisely modify the insect’s genome, CRISPR/Cas9 offers innovative strategies to combat this destructive pest. ...
Upper Bound on the Mutational Burden Imposed by a CRISPR-Cas9 Gene-Drive Element
Tags: CRISPR, Evolution, Genetic biocontrolMichael S. Overton, Sean E. Guy, Xingsen Chen, Alena Martsul, Krypton Carolino, Omar S. Akbari, Justin R. Meyer, Sergey Kryazhimskiy, bioRxiv, 2023.
CRISPR-Cas9 gene drives (CCGDs) are powerful tools for genetic control of wild populations, useful for eradication of disease vectors, conservation of endangered species and other applications. However, Cas9 alone and in a complex with gRNA can cause double-stranded DNA breaks at ...
Assessing the sterility and quality of gamma-irradiated pepper weevils, Anthonomus eugenii (Coleoptera: Curculionidae), toward the development of the sterile insect technique
Tags: Biological control, Pest management, Sterile insect technique (SIT)Basso, J.V., Labbe, R. and Scott-Dupree, C., Pest Management Science, 2023.
The pepper weevil (PW), Anthonomus eugenii, is an economically significant pest of cultivated Capsicum spp. pepper crops in North America where it remains a challenge to manage because of its cryptic immature life stages. The sterile insect technique (SIT) is a genetic pest ...
Procesos celulares modificados en Aedes aegypti infectados con Wolbachia y la susceptibilidad al virus dengue
Tags: Aedes, WolbachiaLópez-Ordóñez T, Díaz-Rodarte KI, Torres-Monzón JA, Casas-Martínez M, Danis-Lozano R, Mosso-González C., Salud Pública de México, 2023.
Objetivo: Analizar la expresión diferencial de proteínas de Aedes aegypti infectados con Wolbachia y su asociación con el ciclo viral del virus dengue (DENV). Material y métodos. Se revisó una base de datos de proteínas de Ae. aegypti infec-tados y no infectados con ...
Side effects of X-ray irradiation on flight ability of Cydia pomonella moth
Tags: Pest management, Sterile insect technique (SIT)Huang, S.-W., Zhang, J.-H., Wei, Z.-H., Yang, X.-M., Wang, X.-Y. and Yang, X.-Q., Pest Management Science, 2023.
The sterile insect technique (SIT) has proven to be an effective approach in managing the population of major invasive pests Our previous studies showed that irradiation of males at a dosage of 366 Gy X-rays resulted in complete sterility. However, the mating competitiveness of ...
Aerial release of Aedes aegypti male mosquitoes using an unmanned aerial vehicle: a novel control strategy
Tags: Aedes, Sterile insect technique (SIT)Valdez-Delgado KM, Ríos-Delgado JC, Nettel-Cruz JA, Angulo-Kladt R, Villarreal-Treviño C., Salud Pública de México, 65. 2023.
Objective. To development of a methodology for the chilling, handling, transport, and release of male Aedes aegypti mosquitoes, reared in insectary conditions to release in the field with unmanned vehicles to compete sexually with wild males in the field. Materials and methods. A ...
The Sterile Insect Technique can efficiently reduce the reproduction of the spotted wing drosophila (Drosophila suzukii) in strawberry
Tags: Pest management, Sterile insect technique (SIT)B. Gard, A. Panel, A. Labbetoul, N. Bosshard, A. Xuereb, B. Cariou, A. Debelle, C. Oliva, S. Fellous, bioRxiv, 2023.
The spotted wing drosophila (SWD) Drosophila suzukii (Diptera: Drosophilidae) is a highly problematic pest in soft fruit crops. Since its introduction in Europe in 2008, no satisfying nor environment-friendly control method has emerged against this species. The sterile insect ...
Population suppression with dominant female-lethal alleles is boosted by homing gene drive
Tags: Fruit fly, Gene drive, Modeling, Pest management, Population suppression, Sterile insect technique (SIT), Synthetic biologyJinyu Zhu, Jingheng Chen, Yiran Liu, Xuejiao Xu, Jackson Champer, bioRxiv, 2023.
Methods to suppress pest insect populations using genetic constructs and repeated releases of male homozygotes have recently been shown to be an attractive alternative to older sterile insect technique based on radiation. Female-specific lethal alleles have substantially ...
The effect of migration on transmission of Wolbachia in Nilaparvata lugens
Tags: WolbachiaZhigang Liu, Tiejun Zhou, Mathematical Biosciences and Engineering, 20. 2023.
Brown planthopper Nilaparvata lugens, which can transmit rice ragged stunt virus, is a serious and damaging pest to rice plants. Rice plants can protect themselves from the associated diseases of N.lugens by either suppressing or replacing N.lugens by releasing N.lugens infected ...
Transformative Approaches for Sustainable Weed Management: The Power of Gene Drive and CRISPR-Cas9
Tags: CRISPR, Gene driveKumam, Y.; Trick, H.N.; Vara Prasad, P.V.; Jugulam, M., Genes, 14. 2023.
Weeds can negatively impact crop yields and the ecosystem’s health. While many weed management strategies have been developed and deployed, there is a greater need for the development of sustainable methods for employing integrated weed management. Gene drive systems can be ...
Viruses that ‘infect’ viruses: Cas12f1 and Cas9 gene drive in HSV1
Tags: Arbovirus, CRISPR, Gene drive, Genetic engineeringHongsheng Dai, Qiaorui Yao, Zhuangjie Lin, Keyuan Lai, Xianyin Zeng, Guangxiong Lei, Tongwen Zhang, bioRxiv, 2023.
Cas9-based synthetic gene drives constitute some minimal elements capable of editing DNA with sequence specificity. However, they face high resistance rate and mitigation strategies developed so far are difficult to implement. Here, we engineered herpes simplex virus type 1 ...
A population modification gene drive targeting both Saglin and Lipophorin impairs Plasmodium transmission in Anopheles mosquitoes
Tags: Anopheles, CRISPR, Gene editing, GeneticsEmily I Green, Etienne Jaouen, Dennis Klug, Roenick Proveti Olmo, Amandine Gautier, Stéphanie Blandin, Eric Marois, eLife, 12. 2023.
Lipophorin is an essential, highly expressed lipid transport protein that is secreted and circulates in insect hemolymph. We hijacked the Anopheles coluzzii Lipophorin gene to make it co-express a single-chain version of antibody 2A10, which binds sporozoites of the malaria ...
Homing-based gene drives can introgress rapidly into local genetic backgrounds with minimal chromosomal conversion in Anopheles gambiae
Tags: Gene editing, Genetic engineeringTony Nolan, Poppy Pescod, Giulia Bevivino et al., Research Square, 2023.
CRISPR-Cas9 gene drive control strategies use a homing selfish genetic element which induces a double-stranded break at the target site and is copied into the opposing chromosome, breaking an essential gene and ensuring super-Mendelian inheritance. During the copying process SNPs ...
Germline transformation of the West Nile Virus and avian malaria vector Culex quinquefasciatus Say using the piggyBac transposon system
Tags: Malaria, Vector controlKatherine Nevard, Rajdeep Kaur, Tim Harvey-Samuel, bioRxiv, 2023.
Culex quinquefasciatus Say is a mosquito which acts as a vector for numerous diseases including West Nile Virus, lymphatic filariasis and avian malaria, over a broad geographical range. As the effectiveness of insecticidal mosquito control methods declines, the need has grown to ...
A homing rescue gene drive with multiplexed gRNAs reaches high frequency in cage populations but generates functional resistance
Tags: CRISPR, Gene drive, Other arthropods, Population modification/replacement, Replicator/site directed nuclease, Synthetic biologyJingheng Chen, Shibo Hou, Ruobing Feng, Xuejiao Xu, Nan Liang, Jackson Champer, bioRxiv, 2023.
CRISPR homing gene drive is a potent technology with considerable potential for managing populations of medically and agriculturally significant insects. It induces a bias in the inheritance of the drive allele in progeny, rapidly spreading desired genes throughout the ...
Reduced dengue incidence following city-wide wMel Wolbachia mosquito releases throughout three Colombian cities: Interrupted time series analysis and a prospective case-control study
Tags: Aedes, WolbachiaVelez ID, Tanamas SK, Arbelaez MP, Kutcher SC, Duque SL, Uribe A, et al., PLoS Neglected Tropical Diseases, 2023.
The introduction of Wolbachia (wMel strain) into Aedes aegypti mosquitoes reduces their capacity to transmit dengue and other arboviruses. Randomised and non-randomised studies in multiple countries have shown significant reductions in dengue incidence following field ...
Large-scale releases and establishment of wMel Wolbachia in Aedes aegypti mosquitoes throughout the Cities of Bello, Medellín and Itagüí, Colombia
Tags: Aedes, Mosquitoes, WolbachiaVelez ID, Uribe A, Barajas J, Uribe S, Ángel S, Suaza-Vasco JD, et al., PLoS Neglected Tropical Diseases, 2023.
The wMel strain of Wolbachia has been successfully introduced into Aedes aegypti mosquitoes and has been shown to reduce the transmission of dengue and other Aedes-borne viruses. Here we report the entomological results from phased, large-scale releases ...
Modelling and analysis of periodic impulsive releases of the Nilaparvata lugens infected with wStri-Wolbachia
Tags: Cytoplasmic incompatibility, WolbachiaXiangjun Dai, Qi Quan and Jianjun Jiao, Journal of Biological Dynamics, 17:1. 2023.
In this paper, we formulate a population suppression model and a population replacement model with periodic impulsive releases of Nilaparvata lugens infected with wStri. The conditions for the stability of wild-N.lugens -eradication periodic solution of two systems are obtained ...
Food for thought: Assessing the consumer welfare impacts of deploying irreversible, landscape-scale biotechnologies
Tags: Fruit fly, Gene driveMichael S. Jones, Zachary S. Brown, Food Policy, 121. 2023.
Genetically engineered insects have gained attention as regionally deployed pest control technologies, with substantial applications in agriculture for combatting intractable crop pests and diseases. One potential tool is a ‘gene drive’, using CRISPR-based gene editing. In ...
Manipulating the Destiny of Wild Populations Using CRISPR
Tags: CRISPR, Gene drive, Genetic biocontrol, Population modification/replacement, Replicator/site directed nucleaseRaban R, Marshall JM, Hay BA, Akbari OS., Annual Reviews, 57:361-390. 2023.
Genetic biocontrol aims to suppress or modify populations of species to protect public health, agriculture, and biodiversity. Advancements in genome engineering technologies have fueled a surge in research in this field, with one gene editing technology, CRISPR, leading the ...
Anopheles gambiae on remote islands in the Indian Ocean: origins and prospects for malaria elimination by genetic modification of extant populations
Tags: Africa, Anopheles, Gene editing, Malaria, Population genetics/dynamicsDitter, R.E., Campos, M., Crepeau, M.W. et al., Scientific Reports, 13. 2023.
The mosquito Anopheles gambiae s.s. is a primary malaria vector throughout sub-Saharan Africa including the islands of the Comoros archipelago (Anjouan, Grande Comore, Mayotte and Mohéli). These islands are located at the northern end of the Mozambique Channel in eastern Africa. ...
Wolbachia Transinfection Via Embryonic Microinjection
Tags: Aedes, Other Symbionts, WolbachiaZhang, M., Xi, Z., Methods in Molecular Biology, 2739. 2023.
The process of transferring Wolbachia from one species to another to establish a stable, maternally inherited infection in the target species is known as transinfection. The success of transinfection is primarily achieved through embryonic microinjection, which is the most direct ...
Measuring Host Fitness Effects and Transmission of Wolbachia Strains in Aedes aegypti Mosquitoes
Tags: Population modification/replacement, Population suppression, WolbachiaRoss, P.A., Methods in Molecular Biology, 2739. 2023.
Lines of Aedes aegypti mosquitoes infected with heritable Wolbachia bacteria are being developed and released for arbovirus control. Coordinated releases of lab-reared Wolbachia mosquitoes have reduced local disease incidence by spreading virus-blocking Wolbachia strains and by ...
Procedures for the Detection of Wolbachia-Conferred Antiviral Protection in Drosophila melanogaster
Tags: Population modification/replacement, WolbachiaChrostek, E., Methods in Molecular Biology, 2739. 2023.
Spread of Wolbachia infections in host populations may be enhanced by Wolbachia-conferred protection from viral pathogens. Wolbachia-infected Drosophila melanogaster survive the pathogenic effects of positive-sense single-stranded RNA virus infections at a higher rate than the ...
Use of Drosophila Transgenics to Identify Functions for Symbiont Effectors
Tags: Fruit fly, Gene editing, WolbachiaCortez, C.T., Murphy, R.O., Owens, I.M., Beckmann, J.F., Methods in Molecular Biology, 2739. 2023.
Wolbachia, one of the most successful and studied insect symbionts, and Drosophila, one of the most understood model insects, can be exploited as complementary tools to unravel mechanisms of insect symbiosis. Although Wolbachia itself cannot be grown axenically as clonal isolates ...
