Scholarly Literature
This is an aggregation of scholarly literature from peer-reviewed journals, bioRxiv , medRxiv and arXiv preprint servers focused on natural and engineered selfish genetic elements and genetic biocontrol. New citations are added as they appear. This content is intended for anyone interested in the scholarly literature on gene drive and genetic biocontrol.
An introgressed gene causes meiotic drive in Neurospora sitophila
Tags: Resistance, Toxin-antidote, Transmission distortion, Yeast and FungiJ. Svedberg, A. A. Vogan, N. A. Rhoades, D. Sarmarajeewa, D. J. Jacobson, M. Lascoux, T. M. Hammond and H. Johannesson, bioRxiv, 2020.01.29.923946. 2020.
In this study, we identify the gene responsible for spore killing in Sk-1 by generating both long and short-read genomic data and by using these data to perform a genome wide association test. By phylogenetic analysis, we demonstrate that the gene is likely to have been ...
First Field Release of a Genetically Engineered, Self-Limiting Agricultural Pest Insect: Evaluating Its Potential for Future Crop Protection
Tags: Gene drive synthetic, Genetic biocontrol, Moths, Oxitec, Self limiting, Sterile insect technique (SIT)A. M. Shelton, S. J. Long, A. S. Walker, M. Bolton, H. L. Collins, L. Revuelta, L. M. Johnson and N. I. Morrison, Frontiers in Bioengineering and Biotechnology, 7:1-15. 2020.
Alternative, biologically-based approaches for pest management are sorely needed and one approach is to use genetically engineered insects. Herein we describe a series of integrated field, laboratory and modeling studies with the diamondback moth, Plutella xylostella, a serious ...
Autosomal suppression and fitness costs of an old driving X chromosome in Drosophila testacea
Tags: Fruit fly, Gene drive, Selfish genetic elements, Sex distorter, Transmission distortion, X chromosomeG. Keais, S. Lu and S. Perlman, Journal of Evolutionary Biology, 2020.
Driving X chromosomes (XDs) are meiotic drivers that bias their own transmission through males by killing Y-bearing gametes. These chromosomes can in theory spread rapidly in populations and cause extinction, but many are found as balanced polymorphisms or as ?cryptic? XDs shut ...
Public Opinion Towards Gene Drive as a Pest Control Approach for Biodiversity Conservation and the Association of Underlying Worldviews
Tags: CRISPR, Ecology, Gene drive synthetic, Genetic biocontrol, Invasive species, Oceania, Policy, Stakeholder engagement, Synthetic biologyE. A. MacDonald, J. Balanovic, E. D. Edwards, W. Abrahamse, B. Frame, A. Greenaway, R. Kannemeyer, N. Kirk, F. Medvecky, T. L. Milfont, J. C. Russell and D. M. Tompkins, Environmental Communication-a Journal of Nature and Culture, 15:1-16. 2020.
Synthetic gene drive approaches are nascent technologies with potential applicability for pest control for conservation purposes. Responsible science mandates that society be engaged in a dialogue over new technology, particularly where there exist global ramifications as with ...
Regulation of GM Organisms for Invasive Species Control
Tags: Biodiversity/Conservation, Ethics, Gene drive synthetic, Gene editing, Invasive species, Policy, Regulation, Risk and safety, Risk assessmentH. J. Mitchell and D. Bartsch, Frontiers in Bioengineering and Biotechnology, 7:1-11. 2020.
Invasive species can cause significant harm to the environment, agriculture, and human health, but there are often very limited tools available to control their populations. Gene drives (GD) have been proposed as a new tool which could be used to control or eliminate such ...
A transcomplementing gene drive provides a flexible platform for laboratory investigation and potential field deployment
Tags: Gene drive mechanisms, Gene drive synthetic, Replicator/site directed nucleaseV. López Del Amo, A. L. Bishop, H. M. Sánchez C, J. B. Bennett, X. Feng, J. M. Marshall, E. Bier and V. M. Gantz, Nature Communications, 11:352. 2020.
CRISPR-based gene drives can spread through wild populations by biasing their own transmission above the 50% value predicted by Mendelian inheritance. These technologies offer population-engineering solutions for combating vector-borne diseases, managing crop pests, and ...
Broad dengue neutralization in mosquitoes expressing an engineered antibody
Tags: Aedes, Dengue, Gene drive synthetic, Population modification/replacementA. Buchman, S. Gamez, M. Li, I. Antoshechkin, H.-H. Li, H.-W. Wang, C.-H. Chen, M. J. Klein, J.-B. Duchemin, J. E. Crowe, Jr., P. N. Paradkar and O. S. Akbari, PLOS Pathogens, 16:e1008103. 2020.
Author summary With limited success of traditional vector control methods to curb dengue infections and more than half of the world’s population still at risk, there is a need for novel strategies to reduce its impact on public health. Recent advances in genetic technologies ...
Development of a confinable gene drive system in the human disease vector Aedes aegypti
Tags: Aedes, CRISPR, Dengue, Gene drive syntheticM. Li, T. Yang, N. P. Kandul, M. Bui, S. Gamez, R. Raban, J. Bennett, H. M. Sánchez C, G. C. Lanzaro, H. Schmidt, Y. Lee, J. M. Marshall and O. S. Akbari, eLife, 9:e51701. 2020.
Aedes aegypti is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become ...
The potential cost-effectiveness of controlling dengue in Indonesia using wMel Wolbachia released at scale: a modelling study
Tags: Aedes, Asia, Dengue, Genetic biocontrol, Genetic incompatibilities, WolbachiaO. J. Brady, D. D. Kharisma, N. N. Wilastonegoro, K. M. Reilly, E. Hendricx, L. S. Bastos, L. Yakob and D. S. Shepard, medRxiv, 2020.01.11.20017186. 2020.
Wolbachia releases in high density urban areas is expected to be highly cost-effective and could potentially be the first cost saving intervention for dengue. Sites with strong public health infrastructure, fiscal capacity, and community support should be prioritized.
Optimal control and analysis of a modified trojan Y-Chromosome strategy
Tags: Biological control, Fish, Genetic biocontrol, Invasive species, Modeling, Population genetics/dynamics, Sex distorterM. A. Beauregard, R. D. Parshad, S. Boon, H. Conaway, T. Griffin and J. J. Lyu, Ecological Modelling, 416. 2020.
The Trojan Y Chromosome (TYC) strategy is a promising eradication method that attempts to manipulate the female to male ratio to promote the reduction of the population of an invasive species. The manipulation stems from an introduction of sex-reversed males, called supermales, ...
Antiviral effectors and gene drive strategies for mosquito population suppression or replacement to mitigate arbovirus transmission by Aedes aegypti
Tags: Aedes, Arbovirus, Gene drive synthetic, Population modification/replacement, Population suppressionA. E. Williams, A. W. E. Franz, W. R. Reid and K. E. Olson, Insects, 11:1-18. 2020.
The mosquito vector Aedes aegypti transmits arthropod-borne viruses (arboviruses) of medical importance, including Zika, dengue, and yellow fever viruses. Controlling mosquito populations remains the method of choice to prevent disease transmission. Novel mosquito control ...
Metaphor, Trust and Support for Non-native Species Control
Tags: Biodiversity/Conservation, Gene drive, Invasive species, Pest management, Policy, Stakeholder engagement, Synthetic biologyP. A. Kohl, S. J. Collins and M. Eichholz, Environmental Communication, 14:672-685. 2020.
This experimental study used a representative sample of U.S. residents (N = 1,042) to test whether the use of the term "invasive" increases support for non-native species control efforts. The term invasive had a small influence on support for two out of three non-native species ...
Wolbachia transinfections in Culex quinquefasciatus generate cytoplasmic incompatibility
Tags: Culex, Cytoplasmic incompatibility, Incompatible insect technique, Population modification/replacement, WolbachiaT. H. Ant, C. Herd, F. Louis, A. B. Failloux and S. P. Sinkins, Insect Molecular Biology, 29:1-8. 2020.
Culex quinquefasciatus is an important mosquito vector of a number of viral and protozoan pathogens of humans and animals, and naturally carries the endosymbiont Wolbachia pipientis, strain wPip. Wolbachia are used in two distinct vector control strategies: firstly, population ...
Scenario analysis on the use of rodenticides and sex-biasing gene drives for the removal of invasive house mice on islands
Tags: Biodiversity/Conservation, Ethics, Gene drive synthetic, Policy, RodentsM. E. Serr, R. X. Valdez, K. S. Barnhill-Dilling, J. Godwin, T. Kuiken and M. Booker, Biological Invasions, 2020.
Since the 1960s conservation efforts have focused on recovering island biodiversity by eradicating invasive rodents. These eradication campaigns have led to considerable conservation gains, particularly for nesting seabirds. However, eradications are complex and lengthy endeavors ...
Beyond Mendelian genetics: Anticipatory biomedical ethics and policy implications for the use of CRISPR together with gene drive in humans.
Tags: Ethics, Gene drive synthetic, Gene editing, Policy, RegulationM. W. Nestor and R. L. Wilson, Journal of Bioethical Inquiry, 2020:1-12. 2020.
Clustered regularly interspaced short palindromic repeats (CRISPR) genome editing has already reinvented the direction of genetic and stem cell research. For more complex diseases it allows scientists to simultaneously create multiple genetic changes to a single cell. ...
Gene drives : equity demands civility
Tags: Biodiversity/Conservation, Gene drive syntheticN. Kofler, Nature, 565:25. 2020.
At the 14th Conference of the Parties of the United Nations Convention on Biological Diversity late last year, I witnessed the rapid deterioration of a crucial discussion. It was on the potential of synthetic biology in environmental conservation. What started as heckling turned ...
Technology Factsheet: Gene Drives
Tags: Gene drive synthetic, Policy, RegulationJ. Lunshof, C. Shachar, R. Edison, A. Jayanti, Belfer Center for Science and International Affairs, 2020.
Gene drives can be defined as genetic elements that pass from parents to unusually high numbers of their offspring due to biased inheritance (sometimes referred to as the possession of “selfish” genetic elements).1,2 There are different ways of achieving this biased ...
A comparison of the Trojan Y Chromosome strategy to harvesting models for eradication of nonnative species
J. J. Lyu, P. J. Schofield, K. M. Reaver, M. Beauregard and R. D. Parshad, Natural Resource Modeling, 2019.The Trojan Y Chromosome strategy (TYC) is a promising eradication method for biological control of nonnative species. The strategy works by manipulating the sex ratio of a population through the introduction of supermales that guarantee male offspring. In the current study, we ...
A typology of community and stakeholder engagement based on documented examples in the field of novel vector control
Tags: Ethics, Gene drive synthetic, Genetic biocontrol, Policy, Risk and safety, Stakeholder engagementC. E. Schairer, R. Taitingfong, O. S. Akbari and C. S. Bloss, PLoS Neglected Tropical Diseases, 13:e0007863. 2019.
Background Despite broad consensus on the importance of community and stakeholder engagement (CSE) for guiding the development, regulation, field testing, and deployment of emerging vector control technologies (such as genetically engineered insects), the types of activities ...
Biomphalaria glabrata Granulin Increases Resistance to Schistosoma mansoni Infection in Several Biomphalaria Species and Induces the Production of Reactive Oxygen Species by Haemocytes
Tags: Gene drive synthetic, Population modification/replacementJ. R. Hambrook, A. A. Gharamah, E. A. Pila, S. Hussein and P. C. Hanington, Genes, 11:12. 2019.
In this study, we demonstrate that in vivo addition of Biomphalaria glabrata pro-granulin (BgGRN) can reduce Schistosoma mansoni infection success in numerous Biomphalaria sp. when challenged with different S. mansoni strains. We also demonstrate that cleavage of BgGRN into ...
Mathematical modeling of self-contained CRISPR gene drive reversal systems
Tags: Gene drive mechanisms, Gene drive syntheticM. G. Heffel and G. C. Finnigan, Scientific Reports, 9:20050. 2019.
There is a critical need for further research into methods to control biological populations. Numerous challenges to agriculture, ecological systems, and human health could be mitigated by the targeted reduction and management of key species (e.g. pests, parasites, and vectors ...
Experimental population modification of the malaria vector mosquito, Anopheles stephensi
Tags: Gene drive mechanisms, Gene drive syntheticT. B. Pham, C. H. Phong, J. B. Bennett, K. Hwang, N. Jasinskiene, K. Parker, D. Stillinger, J. M. Marshall, R. Carballar-Lejarazú and A. A. James, PLOS Genetics, 15:e1008440. 2019.
The experimental introduction of manipulated genes into insect species has a long history in basic genetics. Recent advances in genome editing technologies have spurred considerable effort to exploit these methodologies to provide genetic solutions to some of the worst medical ...
Gene drives for schistosomiasis transmission control
Tags: Gene drive synthetic, Other invertebrates, Risk and safetyT. Maier, N. J. Wheeler, E. K. O. Namigai, J. Tycko, R. E. Grewelle, Y. Woldeamanuel, K. Klohe, J. Perez-Saez, S. H. Sokolow, G. A. De Leo, T. P. Yoshino, M. Zamanian and J. Reinhard-Rupp, PLoS Neglected Tropical Diseases, 13:e0007833. 2019.
Schistosomiasis is one of the most important and widespread neglected tropical diseases (NTD), with over 200 million people infected in more than 70 countries; the disease has nearly 800 million people at risk in endemic areas. Although mass drug administration is a ...
Articulating ‘free, prior and informed consent’ (FPIC) for engineered gene drives
Tags: Ethics, Policy, Risk and safetyGeorge, D. R., T. Kuiken and J. A. Delborne, Proceedings of the Royal Society B: Biological Sciences, 286:20191484.. 2019.
Recent statements by United Nations bodies point to free, prior and informed consent (FPIC) as a potential requirement in the development of engineered gene drive applications. As a concept developed in the context of protecting Indigenous rights to self-determination in land ...
Fitness consequences of a non-recombining sex-ratio drive chromosome can explain its prevalence in the wild
Tags: Evolution, Other arthropods, X chromosomeDyer, K. A. and D. W. Hall, Proceedings of the Royal Society B: Biological Sciences, 286:20192529. 2019.
Understanding the pleiotropic consequences of gene drive systems on host fitness is essential to predict their spread through a host population. Here, we study sex-ratio (SR) X-chromosome drive in the fly Drosophila recens, where SR causes the death of Y-bearing sperm in male ...
Gene drive: progress and prospects
Tags: Evolution, Gene drive mechanisms, Gene drive syntheticWedell, N., T. A. R. Price and A. K. Lindholm, Proceedings of the Royal Society B: Biological Sciences, 286:20192709. 2019.
Gene drive is a naturally occurring phenomenon in which selfish genetic elements manipulate gametogenesis and reproduction to increase their own transmission to the next generation. Currently, there is great excitement about the potential of harnessing such systems to control ...
Ancient gene drives: an evolutionary paradox
Tags: EvolutionPrice, T. A. R., R. Verspoor and N. Wedell, Proceedings of the Royal Society B: Biological Sciences, 286:20192267. 2019.
Selfish genetic elements such as selfish chromosomes increase their transmission rate relative to the rest of the genome and can generate substantial cost to the organisms that carry them. Such segregation distorters are predicted to either reach fixation (potentially causing ...
Transcontinental dispersal of Anopheles gambiae occurred from West African origin via serial founder events
Tags: Anopheles, Gene drive synthetic, Malaria, Population genetics/dynamics, ResistanceH. Schmidt, Y. Lee, T. C. Collier, M. J. Hanemaaijer, O. D. Kirstein, A. Ouledi, M. Muleba, D. E. Norris, M. Slatkin, A. J. Cornel and G. C. Lanzaro, Communications Biology, 2. 2019.
Here we present population genomic analyses of 111 specimens sampled from west to east Africa, including the first whole genome sequences from oceanic islands, the Comoros.
Exterminator genes: The right to say no to ethics dumping
Tags: Ethics, Policy, Risk and safetyBassey-Orovwuje, M., J. Thomas and T. Wakeford, Development, 62:121-127. 2019.
The scientific-industrial complex is promoting a new wave of genetically modified organisms, in particular gene drive organisms, using the same hype with which they tried to persuade society that GMOs would be a magic bullet to solve world hunger. The Gates Foundation claims that ...
A selfish genetic element linked to increased lifespan impacts metabolism in female house mice
Tags: Evolution, Fruit fly, Population genetics/dynamics, Rodents, Transmission distortionLopes, P. C. and A. K. Lindholm, The Journal of Experimental Biology, 2019:212704. 2019.
Gene drive systems can lead to the evolution of traits that further enhance the transmission of the driving element. In gene drive, one allele is transmitted to offspring at a higher frequency than the homologous allele. This has a range of consequences, which generally include a ...
A cross-sectional survey of biosafety professionals regarding genetically modified insects
Tags: Ethics, Other arthropods, Regulation, Risk and safetyO’Brochta, D. A., W. K. Tonui, B. Dass and S. James, Applied Biosafety, 2019:1-9. 2019.
Background:Genetic technologies such as gene editing and gene drive create challenges for existing frameworks used to assess risk and make regulatory determinations by governments and institutions. Insect genetic technologies including transgenics, gene editing, and gene drive ...
The potential for a released autosomal X-shredder becoming a driving-Y chromosome and invasively suppressing wild populations of malaria mosquitoes
Tags: Gene drive mechanisms, Gene drive synthetic, Genetic biocontrol, Malaria, Other arthropods, Replicator/site directed nuclease, Risk and safety, Sex distorter, X chromosome, Y-chromosomeAlcalay, Y., S. Fuchs, R. Galizi, F. Bernardini, R. E. Haghighat-Khah, D. B. Rusch, J. R. Adrion, M. W. Hahn, P. Tortosa and P. A. Papathanos, bioRxiv, 2019:860551. 2019.
Synthetic sex-ratio distorters based on X-chromosome shredding are predicted to be more efficient than sterile males for population suppression of malaria mosquitoes using genetic control. X chromosome shredding operates through the targeted elimination of X-chromosome-bearing ...
Plasmodium falciparum (Haemosporodia: Plasmodiidae) and O’nyong-nyong virus development in a transgenic Anopheles gambiae (Diptera: Culicidae) strain
Tags: Gene drive synthetic, Regulation, Replicator/site directed nuclease, Risk and safety, Y-chromosomeMumford, J. D., C. A. Long, S. C. Weaver, K. Miura, E. Wang, R. Rotenberry, E. M. Dotson and M. Q. Benedict, " Journal of Medical Entomology, 56:936-941. 2019.
ransgenic Anopheles gambiae Giles (Diptera: Culicidae) mosquitoes have been developed that confer sexual sterility on males that carry a transgene encoding a protein which cuts ribosomal DNA. A relevant risk concern with transgenic mosquitoes is that their capacity to transmit ...
Genetically engineering wild mice to combat Lyme disease: An ecological perspective
Tags: Evolution, Gene drive synthetic, Other mammals, RodentsSnow, A. A., BioScience, 69:746-756. 2019.
Genetic engineering of wild populations has been proposed for reducing human diseases by altering pathogens’ hosts. For example, CRISPR- based genome editing may be used to create white-footed mice (Peromyscus leucopus) that are resistant to the Lyme disease ...
What risk assessments of genetically modified organisms can learn from institutional analyses of public health risks
Tags: Regulation, Risk and safetyRajan, S. R. and D. K. Letourneau, Journal of Biomedicine and Biotechnology, 2012:8. 2019.
The risks of genetically modified organisms (GMOs) are evaluated traditionally by combining hazard identification and exposure estimates to provide decision support for regulatory agencies. We question the utility of the classical risk paradigm and discuss its evolution in GMO ...
Design and analysis of CRISPR-based underdominance toxin-antidote gene drives
Tags: CRISPR, Gene drive, Gene drive synthetic, Genetic engineering, Modeling, Population modification/replacement, Population suppression, Toxin-antidote, UnderdominanceChamper, J., S. E. Champer, I. Kim, A. G. Clark and P. W. Messer, bioRxiv, 861435:861435. 2019.
CRISPR gene drive systems offer a mechanism for transmitting a desirable transgene throughout a population for purposes ranging from vector-borne disease control to invasive species suppression. In this simulation study, we model and assess the performance of several CRISPR-based ...
Disrupting female flight in the vector Aedes aegypti
Tags: Gene drive mechanisms, Gene drive synthetic, MosquitoesO'Leary, S. and Z. N. Adelman, bioRxiv, 862300:862300. 2019.
Aedes aegypti is a vector of dengue, chikungunya, and Zika viruses. Current vector control strategies such as community engagement, source reduction, and insecticides have not been sufficient to prevent viral outbreaks. Thus, interest in novel strategies involving genetic ...
Maintenance of fertility in the face of meiotic drive
Tags: Evolution, Other arthropods, Transmission distortionMeade, L., S. Finnegan, R. Kad, K. Fowler and A. Pomiankowsk, The American Naturalist, 2019:2019. 2019.
Selfish genetic elements that gain a transmission advantage through the destruction of sperm have grave implications for drive male fertility. In the X-linked SR meiotic drive system of a stalk-eyed fly, we found that drive males have greatly enlarged testes and maintain high ...
The analytical review on futuristic use of CRISPR-Cpf1 aided gene drive technology
Tags: Gene drive syntheticSatyam, R., N. Singh and T. Bhardwaj, International Journal of Trend in Scientific Research and Development, 2:525-530. 2019.
Organisms edited by gene editing or traditional selective breeding are typically less able to survive and reproduce which effectively prevents those alterations from spreading in the wild populations. This paper focuses on how the CRISPR-Cpf1 system can be used to build a Gene ...
An assessment of the immune costs associated with meiotic drive elements in Drosophila
Tags: Evolution, Other arthropods, Transmission distortionLea, J. K. and R. L. Unckless, Proceedings of the Royal Society B: Biological Sciences, 286:20191534. 2019.
Most organisms are constantly adapting to pathogens and parasites that exploit their host for their own benefit. Less studied, but perhaps more ubiquitous, are intragenomic parasites or selfish genetic elements. These include transposable elements, selfish B chromosomes and ...
Invasive insects: Management methods explored
Tags: Gene drive synthetic, Other arthropodsMcLaughlin, G. M. and P. K. Dearden, Journal of Insect Science, 19:1-9. 2019.
Invasive insect species can act as a plague across the globe, capable of vast expansion and rapid, proliferate reproduction. The spread of pathogens of serious diseases such as malaria and Zika virus and damages to agricultural crops number some of the afflictions invasive ...
Winning the tug-of-war between effector gene design and pathogen evolution in vector population replacement strategies
Tags: Evolution, Gene drive, Gene drive synthetic, Vector controlMarshall, J. M., R. R. Raban, N. P. Kandul, J. R. Edula, T. M. León and O. S. Akbari, Frontiers in Genetics, 10:1072. 2019.
While efforts to control malaria with available tools have stagnated, and arbovirus outbreaks persist around the globe, the advent of clustered regularly interspaced short palindromic repeat (CRISPR)-based gene editing has provided exciting new opportunities for genetics-based ...
Identification and characterisation of a Masculinizer homolog in the diamondback moth Plutella xylostella
Tags: Gene drive, Gene drive mechanisms, Gene drive synthetic, Moths, Other arthropods, Population suppressionHarvey-Samuel, T., V. C. Norman, R. Carter, E. Lovett and L. Alphey, Insect Molecular Biology, 2019:2019. 2019.
Recently, a novel sex-determination system was identified in the silkworm (Bombyx mori) in which a piRNA encoded on the female-specific W chromosome silences a Z-linked gene (Masculinizer) which would otherwise initiate male sex-determination and dosage compensation. ...
A novel drug-inducible sex separation technique for insects
Tags: Gene drive synthetic, Other arthropodsKandul, N. P., J. Liu, A. D. Hsu, B. A. Hay and O. S. Akbari, bioRxiv, 2019:2019.12.13.875716. 2019.
Large sterile male releases are the gold standard for most insect population control methods and thus precise sex sorting is essential to the success of these technologies. Sex sorting is especially important for mosquito control because female mosquitoes bite and transmit ...
Gene drive and resilience through renewal with next generation Cleave and Rescue selfish genetic elements
Tags: CRISPR, Fruit fly, Gene drive, Gene drive synthetic, Selfish genetic elements, Toxin-antidote, UnderdominanceOberhofer, G., T. Ivy and B. A. Hay, bioRxiv, 2019:2019.2012.2013.876169. 2019.
Gene drive-based strategies for modifying populations face the problem that genes encoding cargo and the drive mechanism are subject to separation, mutational inactivation, and loss of efficacy. Resilience, an ability to respond to these eventualities in ways that restore ...
A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus
Tags: Gene drive synthetic, Other SymbiontsValderrama, J. A., S. S. Kulkarni, V. Nizet and E. Bier, Nature Communications, 10:5726. 2019.
Gene-drive systems in diploid organisms bias the inheritance of one allele over another. CRISPR-based gene-drive expresses a guide RNA (gRNA) into the genome at the site where the gRNA directs Cas9-mediated cleavage. In the presence of Cas9, the gRNA cassette and any linked cargo ...
Development of genetic control strategies for insect pests using CRISPR/Cas9 Développement de méthodes de lutte génétique contre de l’insecte nuisible basé sur le system CRISPR/Cas9
Tags: Anopheles, CRISPR, Gene drive synthetic, Gene editingE. Green, Université de Strasbourg, 2019.
nsect pest control remains an important economic, environmental, and public health challenge. CRISPR/Cas9 gene drive (GD) is a novel genetic control strategy. GDs are genetic systems that can rapidly invade a population. This manuscript presents my efforts to develop gene drives ...
Genetic Control of Mosquitoes
Tags: Gene drive mechanisms, Gene drive synthetic, Mosquitoes, Oxitec, Sterile insect technique (SIT), Toxin-antidote, Transmission distortion, Underdominance, X chromosome, Y-chromosomeAlphey, L., Annual Review of Entomology, 59:205-224. 2019.
Genetics can potentially provide new, species-specific, environmentally friendly methods for mosquito control. Genetic control strategies aim either to suppress target populations or to introduce a harm-reducing novel trait. Different approaches differ considerably in their ...
Community Engagement Prior to a Small-Scale Pilot of the Sterile Insect Technique in Kwazulu-Natal, South Africa 2018
Tags: Africa, Anopheles, Stakeholder engagement, Sterile insect technique (SIT)P. N. Manana, J. Zikhali, D. Dlamini, S. Gumede, N. Mabaso, T. Mpungose and G. Munhenga, Journal of Public Health and Disease Prevention, 2. 2019.
Approximately 165 000 listeners were engaged during two 30 minute radio interviews at a local radio station. Two hundred and fifty farm workers, several outpatients from primary health care facilities and 1400 secondary school pupils were given education on malaria transmission ...
Mass-Rearing of Drosophila suzukii for Sterile Insect Technique Application: Evaluation of Two Oviposition Systems
Tags: Fruit fly, Genetic biocontrol, Sterile insect technique (SIT)F. Sassù, K. Nikolouli, S. Caravantes, G. Taret, R. Pereira, M. J. B. Vreysen, C. Stauffer and C. Cáceres, Insects, 10. 2019.
Drosophila suzukii (Diptera: Drosophilidae) is an invasive pest of a wide range of commercial soft-skinned fruits. To date, most management tactics are based on spraying of conventional and/or organic insecticides, baited traps, and netting exclusion. Interest has been expressed ...
