Scholarly Literature
This is an aggregation of scholarly literature from peer-reviewed journals, bioRxiv , medRxiv and arXiv preprint servers focused on natural and engineered selfish genetic elements and genetic biocontrol. New citations are added as they appear. This content is intended for anyone interested in the scholarly literature on gene drive and genetic biocontrol.
Experimental Test of Evolutionary Safety of a CRISPR-Cas9 Gene-Drive Element
Tags: CRISPR, Gene drive, Risk and safetyMichael S Overton, Sean E Guy, Xingsen Chen, et al., bioRxiv, 2025.
CRISPR-Cas9 gene drives (CCGDs) are powerful tools for genetic control of wild populations, with applications from disease eradication to species conservation. However, Cas9 alone and in a complex with gRNA can cause double-stranded DNA breaks at off-target sites, which could ...
Synthetically Assisted Conservation and the Application of Emerging Biological Technologies for the Protection of Biodiversity
Tags: Agriculture, Biodiversity/Conservation, Pest management, Synthetic biologyJedediah F. Brodie, Amanda Emmel, Blake Wiedenheft, et al., Conservation Letters, 8. 2025.
New tools of synthetic biology that enable precise manipulation of genomes, metabolic pathways, and ecosystems present new opportunities, risks, ethical dilemmas, and responsibilities for stewards of biodiversity. We argue that the risks and benefits of synthetic biology for use ...
Integrated vector management with the sterile insect technique component for the suppression of Aedes aegypti in an urban setting in Indonesia
Tags: Aedes, Asia, Population suppression, Vector controlSasmita HI, Neoh K-B, Ernawan B, Indarwatmi M, Nasution IA, Fitrianto N, et al., PLoS Neglected Tropical Diseases, 2025.
The sterile insect technique (SIT) involves subjecting laboratory-bred male mosquitoes to radiation, typically gamma rays, X-rays, or electrons, that render them sterile. These sterile male mosquitoes are then released into the field to mate with wild female mosquitoes. From that ...
Extended time to maturity in Anopheles coluzzii: Implications of late egg hatch for vector control and transgene fitness
Tags: Anopheles, Vector controlEmmanuel C. Ottih, Joe M. Roberts, Toby J. A. Bruce, Frédéric Tripet, Medical and Veterinary Entomology, 2025.
Maintaining fitness is an important consideration when mosquitoes are mass-reared for the deployment of genetic interventions that are designed to suppress populations because released mosquitoes need to compete with wild-type mosquitoes. Late-hatching mosquitoes are more ...
A male-drive female-sterile system for the self-limited control of the malaria mosquito Anopheles gambiae
Tags: Anopheles, Genetically modified mosquitoes, MosquitoesAnna Strampelli, Katie Willis, Hannah Robyn Gulliford, et al., bioRxiv, 2025.
The use of insecticides and antimalarial drugs has been crucial in reducing the mortality and morbidity associated with malaria. However, since 2015, several challenges, including the development of resistance to these insecticides and treatments and changes in mosquito ...
The B Chromosome of Pseudococcus viburni: A Selfish Chromosome that Exploits Whole-Genome Meiotic Drive
Tags: Chromosomal drive, Genomics, Selfish genetic elementsIsabelle M Vea, Andrés G de la Filia, Kamil S Jaron, Scott E J Barlow, Marion Herbette, Andrew J Mongue, Ross Nelson, Francisco J Ruiz-Ruano, Laura Ross, Genome Biology and Evolution, 17. 2025.
Meiosis is generally a fair process: each chromosome has a 50% chance of being included into each gamete. However, meiosis can become aberrant with some chromosomes having a higher chance of making it into gametes than others. Yet, why and how such systems evolve remains unclear. ...
Changes in the frequency of facultative endosymbionts in insect populations: overview and applications
Tags: Genetic biocontrol, Genetic incompatibilities, WolbachiaHoffmann, Ary A.; Cooper, Brandon S., Entomologia Generalis, 45:351-368. 2025.
Many insect endosymbionts are facultative from the host perspective, and their population frequencies across time and space will depend on their transmission fidelity and effects on host fitness. These effects and transmission rates in turn depend on the environmental and host ...
Mozzie: a computationally efficient simulator for the spatio-temporal modelling of mosquitoes
Tags: Anopheles, Modeling, Mosquitoes, Risk assessmentWilkins et al., Journal of Open Source Software, 10. 2025.
Mozzie enables simulation of the lifecycle and spatial spread of mosquitoes. Mozzie can be used to assess risks associated with disease-control strategies at local, regional or continental scales. Most particularly, strategies involving genetic alterations of mosquitoes to ...
Modelling and sensitivity analysis to control dengue through inclusion of genetically modified hybrid mosquitoes
Tags: Dengue, Modeling, Mosquitoes, Sterile insect technique (SIT)Wasim, T., Ismaeel, T., Naseem, A., Abdeljawad, T., & Alqudah, M. A., Mathematical and Computer Modelling of Dynamical Systems, 31. 2025.
Dengue fever, an ancient yet persistent global health threat transmitted by mosquitoes, remains difficult to control. Recent advances propose genetically modified hybrid mosquitoes as a novel intervention. This study focuses on two primary control strategies: the Sterile Insect ...
Influence of genetic factors of humans, mosquitoes and parasites, on the evolution of Plasmodium falciparum infections, malaria transmission and genetic control methods: a review of the literature
Tags: Genetics, Malaria, Resistance, Transmission distortionNikiema, S., Soulama, I., Ampofo, G.D. et al., BMC Medical Genomics, 18. 2025.
Despite significant progress, malaria remains a public health problem in many regions, particularly in sub-Saharan Africa. This situation is partly explained by the mosquito’s resistance to insecticides and the emergence of parasite resistance to antimalarial drugs. Indeed, in ...
Conflicting Kinesin-14s in a single chromosomal drive haplotype
Tags: Chromosomal drive, Selfish genetic elementsMeghan J Brady, Anjali Gupta, Jonathan I Gent, et al., Genetics, 2025.
In maize, there are 2 meiotic drive systems that target large heterochromatic knobs composed of tandem repeats known as knob180 and TR-1. The first meiotic drive haplotype, abnormal chromosome 10 (Ab10) confers strong meiotic drive (∼75% transmission as a heterozygote) and ...
Evaluating the performance of three packing methods for long-distance transport of sterile adult Aedes albopictus males
Tags: Aedes, Mosquitoes, Sterile insect technique (SIT)Mamai, W., Puggioli, A., Wallner, T. et al., Scientific Reports, 15. 2025.
This study evaluated the performance of three packing methods for long distance transport of sterile adult Aedes albopictus males, considering the specific effects of pupal vs. adult irradiation and two compaction methods. The results indicated that while transportation ...
Intrinsically weak sex chromosome drive through sequential asymmetric meiosis
Tags: Chromosomal drive, Fruit fly, Selfish genetic elementsXuefeng Meng, Yukiko M. Yamashita, Science Advances, 11. 2025.
Meiotic drivers are selfish genetic elements that bias their own transmission, violating Mendel’s Law of Equal Segregation. It has long been recognized that sex chromosome–linked drivers present a paradox: Their success in transmission can severely distort populations’ sex ...
Efficient CRISPR-Cas9-mediated genome editing of the cane toad (Rhinella marina)
Tags: CRISPR, Gene editing, Pest managementMichael Clark, Alexander T. Funk, Alex Paporakis, et al., bioRxiv, 2025.
Invasive species inflict major ecological, economic, social, and cultural harm worldwide, highlighting the urgent need for innovative and effective control strategies. Genome editing offers exciting possibilities for creating highly targeted control methods for invasive species. ...
An animal toxin-antidote system kills cells by creating a novel cation channel
Tags: Toxin-antidoteCaro L, Wei AD, Thomas CA, Posch G, Uremis A, Franzi MC, et al., PLoS Biology, 23. 2025.
Toxin-antidote systems are selfish genetic elements composed of a linked toxin and antidote. The peel-1 zeel-1 toxin-antidote system in C. elegans consists of a transmembrane toxin protein PEEL-1 which acts cell autonomously to kill cells. Here we investigate the molecular ...
Applying the Protective Precautionary Principle to the Ethical Use of Gene Drive Technology for Anopheles gambiae Suppression in Malaria Control
Tags: Anopheles, Ethics, Gene drive, Population suppression, Risk and safetyNucharee Wongsamut, Journal of Applied Animal Ethics Research, 2025.
Malaria contributes to poverty and illness, which further hinder productivity and income generation. Therefore, combating malaria is crucial for breaking the vicious cycle of poverty. Genome editing technologies, such as gene drives designed to suppress Anopheles ...
The challenge of measuring mosquito flight performance: going beyond sterile insect technique and into transgenic and gene drive-based approaches
Tags: Gene drive, Sterile insect technique (SIT), Synthetic biologyPaola Najera, Christian E. Ogaugwu, Tyler F. Chan, et al., Open Biology, 15. 2025.
Invasive insects inflict global costs of more than 70 billion USD annually by destroying crops and spreading disease-causing pathogens. Sterile insect technique (SIT), an insect population control method, involves the irradiation or chemical sterilization of insects to produce ...
Paratransgenesis: Overview, Current Perspectives, and Future Research Needs for Malaria Control
Tags: Malaria, Other invertebrates, Vector controlOziegbe, O., Okeke, C.C., Esho, D.O., Springer, Cham., 2025.
Malaria is an insect-borne disease (IBD) that is responsible for significant human mortality and morbidity globally. Several effective vector and parasite control strategies have been considered to control malaria. However, paratransgenesis is a strategy targeted towards parasite ...
Assessing the population genetic structure and demographic history of Anopheles gambiae and An. arabiensis at island and mainland populations in Uganda: Implications for testing novel malaria vector control approaches
Tags: Africa, Anopheles, Genetic engineering, Malaria, Vector controlRita Mwima, Tin-Yu J. Hui, Edward Lukyamuzi, et al, bioRxiv, 2025.
This study collected 2918 Anopheles gambiae and 173 Anopheles. arabiensis across six populations from both the islands on Lake Victoria and mainland Uganda for amplicon sequencing. Large pairwise FST values were observed between the two species, indicating their divergence. We ...
Optimizing larval mass-rearing techniques for Aedes mosquitoes: enhancing production and quality for genetic control strategies
Tags: Aedes, Genetic biocontrol, Mosquitoes, Sterile insect technique (SIT)Wadaka Mamai, Cécile Brengues, Hamidou Maiga, et al, Parasite, 32. 2025.
The quantity and quality of laboratory-reared insects are pivotal for the success of any sterile male-release program. Optimizing larval mass-rearing methods to enhance both production and quality in Aedes mosquitoes is essential to meet the growing demand from FAO/IAEA Member ...
Unstable laboratory Wolbachia strain w-Anga is negatively correlated with Plasmodium falciparum in wild malaria vectors
Tags: Anopheles, Malaria, WolbachiaEstelle, D.L., Jacques, G.E., Issiaka, S. et al., Scientific Reports, 15. 2025.
Spread of insecticides resistance threatens the control of malaria. In this context, biological control using an endosymbiotic bacterium Wolbachia is being explored as a complementary method for its control. However, for optimal use of this bacterium in biocontrol strategies, it ...
Suppression of Aedes mosquito populations with the boosted sterile insect technique in tropical and Mediterranean urban areas
Tags: Aedes, Europe, Invasive species, Sterile insect technique (SIT), Vector controlBouyer, J., Gil, D.A., Mora, I.P. et al., Scientific Reports, 15. 2025.
Aedes mosquitoes are the vectors of dengue viruses and other arboviruses, which threaten billions of people all over the world. The boosted sterile insect technique (boosted SIT) is a version of SIT in which irradiated sterile males also transmit a biocide to immature stages. We ...
A male-transmitted B chromosome undergoes strong meiotic drag in females
Tags: Chromosomal drive, Selfish genetic elementsPatrick M Ferree, Jayla Cummings, Emma Garman, et al, bioRxiv, 2025.
Many organisms carry extra, non-essential chromosomes known as B chromosomes (Bs), which are selfishly transmitted at super-Mendelian levels to offspring. This heightened transmission, termed drive, occurs during gametogenesis, usually in one of the two parents. In some cases, Bs ...
Evaluating the impact on F1 sterility of tsetse pupae Glossina morsitans morsitans irradiated following short-term hypoxic conditioning
Tags: Irradiation method, Sterile insect technique (SIT)Caroline K. Mirieri, Kiswend-sida M. Dera, Vanessa S. Dias, et al, Insect Science, 2025.
The sterile insect technique (SIT) for tsetse involves releasing sterilized males to outcompete wild males in mating, resulting in nonviable progeny. Balancing optimum sterility and male quality is crucial. While irradiation in hypoxia or anoxia is routine for tephritid flies, ...
Spiroplasma infection in colonized Glossina fuscipes fuscipes: impact on mass rearing and the sterile insect technique
Tags: Sterile insect technique (SIT), Vector controlKiswend-sida M. Dera, Daouda Tande Barro, Bénéwendé Aristide Kaboré, et al, Insect Science, 2025.
Tsetse flies (Glossina spp.) can vector the parasites (Trypanosoma spp.) that cause the socioeconomically devastating neglected tropical diseases human and animal African trypanosomoses. In addition to this parasite, tsetse can harbor four genera of endosymbiotic bacteria, ...
Deliberate extinction by genome modification: An ethical challenge
Tags: Biodiversity/Conservation, EthicsGregory E. Kaebnick et al., Science, 388:707-709. 2025.
Among the ways that genome modification could be used to modify wild populations of organisms, the deliberate outcome of fully eradicating a species has received little critical attention. The lack of discussion leaves a difficult ethical question unresolved: When so much ...
Biased tertiary sex ratios enhance the efficacy of sex-ratio distorting genetic techniques to control invasive species
Tags: Genetic biocontrol, Invasive species, Risk assessment, Sex distorterMichael L. Jones, Ronald E. Thresher, Nicholas J. Bax, Journal of Theoretical Biology, 2025.
Genetic biocontrol strategies are increasingly being developed and tested for reducing the effects of invasive species, and are highly likely to be an important tool of integrated pest management in the future. Included among such strategies are those that distort the sex ratio ...
Overview of the sterile insect technique for Aedes aegypti in Lee County, Florida, USA
Tags: Aedes, North America, Sterile insect technique (SIT), Vector controlMorreale, R., Stenhouse, S., Bajonero, J. et al., Infectious Diseases of Poverty, 14. 2025.
Lee County Mosquito Control District (LCMCD) is an independent taxing district that works to protect human health and improve quality of life in Lee County, Florida, USA. With local dengue transmission in southern Florida, LCMCD prioritized the control of Aedes aegypti. Due to ...
Differential elimination of marked sex chromosomes enables production of nontransgenic male mosquitoes in a single strain
Tags: Aedes, Mosquitoes, Sex distorterA. Compton, A. Sharma, M. Hempel, A. Aryan, J.K. Biedler,M.B. Potters, K. Chandrasegaran, C. Vinauger, & Z. Tu, Proceedings of the National Academy of Sciences, 122. 2025.
Diverse genetic strategies are being pursued to control mosquito-borne infectious diseases. These strategies often rely on the release of nonbiting males to either reduce the target mosquito population or render them resistant to pathogens. Male-only releases are important as any ...
Functional validation of a white pupae minimal gene construct in Ceratitis capitata
Tags: Sterile insect technique (SIT), X chromosomeLucas Henrique Figueiredo Prates, Roswitha A. Aumann, Inga Sievers, et al., Insect Science, 2025.
Genetic sexing strains (GSS) are important tools for the sterile insect technique (SIT), an environmentally friendly and species-specific insect pest control method. GSS feature sex-specific phenotypes, enabling sex sorting in mass-rearing facilities and male-only releases, which ...
Nanobody-mediated targeting of Plasmodium falciparum PfPIMMS43 can block malaria transmission in mosquitoes
Tags: Anopheles, Malaria, MosquitoesUkegbu, C.V., Mohamed, M., Hoermann, A. et al., Communications Biology, 8. 2025.
The transition from ookinete to oocyst is a critical step in the Plasmodium falciparum lifecycle and an important target for malaria transmission-blocking strategies. PfPIMMS43, a surface protein of P. falciparum ookinetes and sporozoites, is critical for this transition and ...
Maximising Eradication Potential of Rat Gene Drives Using a Two-Target Homing Rescue Strategy: Spatial Modelling of Empirical Data
Tags: Biodiversity/Conservation, Ecology, Gene drive, Modeling, RodentsBirand, A., Gierus, L., Prowse, T., Cassey, P., Thomas, P.Q., Molecular Ecology, 2025.
Gene drives are genetic elements with positively biased transmission and may be useful tools to suppress mammalian pests that threaten biodiversity worldwide. While gene drives are progressing in mice, less is known about their potential for invasive rat control. A recent report ...
Impact of sterile Aedes aegypti males releases on vector dynamics: insights from Malaysian field trials
Tags: Aedes, Asia, Field trials, Sterile insect technique (SIT)Nazni, W.A., Teoh, GN., Nuradila, M.A. et al., Infectious Diseases of Poverty, 14. 2025.
The Sterile insect technique (SIT) has been successfully used in agricultural pest control, leading to interest in its application for public health, particularly in controlling Aedes mosquitoes in the USA, Italy, Cuba, and Greece. Malaysia has conducted a small-scale SIT pilot ...
Economic evaluation of Wolbachia deployment in Colombia: A modeling study
Tags: Modeling, Policy, South/Central America, WolbachiaShepard, D.S., Lee, S.R., Halasa-Rappel, Y.A., Rincon Perez, C.W., Harker Roa, A., PLoS One, 20. 2025.
Wolbachia are bacteria that inhibit dengue virus replication within the mosquito. A cluster-randomized trial in Indonesia found Wolbachia reduced virologically-confirmed dengue cases by 77.1%. Previous models predicted Wolbachia to be highly cost-effective in Indonesia, ...
Optimizing Cost-Effective Larval Diets for Mass Rearing of Aedes Mosquitoes in Vector Control Programs
Tags: Aedes, Vector controlLi, Q., Wei, T., Sun, Y., Khan, J., & Zhang, D., Insects, 16. 2025.
Larval diet composition significantly influences the developmental, physiological, and reproductive traits of Ae. albopictus and Ae. aegypti, major arbovirus vectors. Optimizing larval nutrition is essential for mass-rearing programs supporting the sterile insect technique and ...
A selfish supergene causes meiotic drive through both sexes in Drosophila
Tags: Fruit fly, Selfish genetic elements, X chromosomeG.L. Keais,C.M. Saad-Roy,E. Gonzalez-Sqalli, et al., Proceedings of the National Academy of Sciences, 122. 2025.
Meiotic drivers are selfish genetic elements that bias their own transmission during meiosis or gamete formation. Due to the fundamental differences between male and female meiosis in animals and plants, meiotic drivers operate through distinct mechanisms in the two sexes: In ...
Optimization of SgRNA expression with RNA pol III regulatory elements in Anopheles stephensi
Tags: Anopheles, CRISPR, Genetic biocontrol, Malaria, MosquitoesGonzalez, E., Anderson, M.A.E., Ang, J.X.D. et al., Scientific Reports, 15. 2025.
Anopheles stephensi, a major Asian malaria vector, is invading Africa and has been implicated in recent outbreaks of urban malaria. Control of this species is key to eliminating malaria in Africa. Genetic control strategies, and CRISPR/Cas9-based gene drives are emerging as ...
Stronger population suppression by gene drive targeting doublesex from dominant female-sterile resistance alleles
Tags: CRISPR, Gene drive, Population suppression, ResistanceWeizhe Chen, Ziye Wang, Jackson Champer, bioRxiv, 2025.
CRISPR homing drives can be used to suppress a population by targeting female fertility genes. They convert wild-type alleles to drive alleles in the germline of drive heterozygotes by homology-directed repair after DNA cleavage. However, resistance alleles produced by ...
An eco-epidemiological model for malaria with Microsporidia MB as bio-control agent
Tags: Anopheles, Modeling, Other Symbionts, Transmission distortionMfangnia CNT, Tonnang HEZ, Tsanou B, Keith Herren J, Modeling Earth Systems and Environment, 11. 2025.
Microsporidia MB is an endosymbiont which naturally infects Anopheles mosquitoes. Due to its ability to block Plasmodium transmission, it shows potential as a bio-based agent for the control of malaria. Its self-sustainability is promising, as it can spread through both ...
International perspectives on the meaning of engagement in biotechnology risk assessment: the case of gene drive
Tags: Gene drive, Risk assessment, Stakeholder engagementHartley, S., Stelmach, A., Kokotovich, A., & Smith, R. D. J., Journal of Risk Research, 2025.
Biotechnology remains challenging for risk assessors. The risk assessment of genetically modified organisms was the locus of considerable, acrimonious, and politicised debate and subjected to intense scrutiny. Since then, biotechnology has evolved to include new tools, such as ...
Mathematically modelling the population dynamics of CRISPR gene drive systems in the pine pest Sirex noctilio
Tags: CRISPR, Gene drive, Modeling, Pest management, Population genetics/dynamicsStrydom, H., Ouifki, R., Chapwanya, M., bioRxiv, 2025.
Sirex noctilio is an invasive pest of pine that has caused significant economic damage in South Africa and many other Southern Hemisphere countries. Current management tools are not efficient in all cases and consequently there is a need for more efficient and targeted control ...
Evolutionary consequences of long-distance dispersal in mosquitoes
Tags: Modeling, Mosquitoes, Population genetics/dynamicsThomas L Schmidt, Current Opinion in Insect Science, 68. 2025.
Long-distance dispersal (LDD) provides a means for mosquitoes to invade new regions and spread adaptive alleles, including those conferring insecticide resistance. Most LDD takes place on human transport vessels and will typically be rarer and more directionally constrained than ...
Applications and status of gene drive in plants
Tags: Ecology, Fruit fly, Invasive species, Population genetics/dynamics, Toxin-antidoteHay BA, PLOS Biology, 23. 2025.
Globalization—the movement of humans, plants, animals, and microbes—together with climate change, place numerous plant and animal species under novel stresses. For plants, threats include global warming, invasive species, weeds, animal pests, and vectors of disease. Harms can ...
Problem formulation for a small-scale field study of non-gene drive, genetically modified, male bias mosquitoes in Burkina Faso
Tags: Africa, Genetic biocontrol, Malaria, Risk assessment, Target malariaHayes, K. R., & Hosack, G. R., Human and Ecological Risk Assessment: An International Journal, 2025.
This article documents the problem formulation step of a risk assessment for a proposed small-scale field release in Burkina Faso of genetically modified (GM) An. coluzzii mosquitoes that carry a Paternal Male Bias (PMB) construct. These mosquitoes are part of the pathway ...
Synthetic Homing Endonuclease Gene Drives to Revolutionise Aedes aegypti Biocontrol – Game Changer or Pipe Dream?
Tags: Aedes, Gene drive synthetic, Vector controlJoshua X.D. Ang, Sebald A.N. Verkuijl, Michelle A.E. Anderson, Luke Alphey, Current Opinion in Insect Science, 2025.
The increasing burden of Aedes aegypti-borne diseases, particularly dengue, is a growing global concern, further exacerbated by climate change. Current control strategies have proven insufficient, necessitating novel approaches. Synthetic homing endonuclease gene (sHEG) drives ...
An innovation in host responses to escalating genomic conflicts
Tags: Fruit fly, Genomics, Selfish genetic elementsMartí, Emiliano et al., Trends in Genetics, 2025.
Conflicts between selfish elements and their hosts can trigger rapid structural and regulatory changes in genomes. Chen et al. discovered a novel species-specific innovation in response to a meiotic driver in Drosophila melanogaster. Their discovery highlights a new dimension in ...
Anticipating evolutionary responses of mosquito mating systems to population suppression with mass-reared males
Tags: Evolution, Modeling, Mosquitoes, Population suppressionBonsall, M.B., Cator, L.J., Current Opinion in Insect Science, 68. 2025.
Strategies that rely on the mass release of males to suppress mosquito populations will exert selective pressure on natural mating systems. Here, we investigate how mass releases might affect the mating behaviors of wild target populations. We highlight gaps in our understanding ...
Wolbachia: A bacterial weapon against dengue fever- a narrative review of risk factors for dengue fever outbreaks
Tags: Dengue, Risk and safety, WolbachiaSafaei, S., Derakhshan-Sefidi, M., Karimi, A., New Microbes and New Infections, 65. 2025.
Arboviruses constitute the largest known group of viruses and are responsible for various infections that impose significant socioeconomic burdens worldwide, particularly due to their link with insect-borne diseases. The increasing incidence of dengue fever in non-endemic regions ...
Comparison of oviposition and adult trapping to monitor wMel introgression for Wolbachia-based vector control
Tags: Aedes, Vector control, WolbachiaNelson, E., Pereira, T.N., Ribeiro E., et al., bioRxiv, 2025.
Wolbachia introgression into Aedes aegypti mosquito populations has been shown to be effective in preventing dengue and is being evaluated for WHO prequalification. Monitoring the long-term introgression of Wolbachia (wMel)-positive Aedes aegypti mosquitoes, however, requires ...
Mosquito Sex Separation using Complementation of Selectable Traits and Engineered Neo-Sex Chromosomes
Tags: Aedes, CRISPRZaada, D.S.Y., Toren, O., Krsticevic, F., et al., bioRxiv, 2025.
Effective and scalable sex separation remains a critical challenge for mosquito genetic control strategies. Genetic sexing strains (GSS) address this by genetically linking maleness with selectable traits, enabling efficient removal of females before release. Here, we describe a ...
