Keywords: Aedes

‘A Jurassic Park Experiment’: Watchdog Groups Denounce Decision to Release Genetically Modified Mosquitoes in Florida

L. Newcomb,  Common Dreams,  2020.
Food safety and environmental groups Wednesday condemned a decision by officials in Florida to approve the release of 750 million genetically modified mosquitoes, a pilot project aimed at reducing the spread of mosquito-borne diseases.
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‘Home to GMO Mosquitoes?!’ Florida Unleashes a Billion Lab Grown Mosquitoes

Anonymous,  B and T MAGAZINE,  2021.
Up to a billion genetically engineered mosquitoes are going to be released in the Florida Keys—but locals are having none of it, with billboards erected saying ‘no!’ to the damned blood suckers. The genetically-modified mosquitoes, known as the OX5034, were made in a ...
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‘Clever Approach’: Scientists Create GM-Free Organisms Using Genetic Engineering

A. Paleja,  The WIRE,  2021.
Farther to the north, researchers at the University of Minnesota have developed a novel way to resolve this problem. They used genetic engineering to create organisms for release that are not genetically modified. Maciej Maselko was a postdoctoral associate at the university ...
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‘Just Add Water’ GM Mosquitoes Suppress Wild Population by 95%

H. Alber,  ,  2020.
In order to reduce the spread of dengue, Oxitec releases genetically modified male Aedes mosquitoes into the environment. Once in the wild, these insects breed with local female mosquitoes and pass on a gene that causes female offspring to die at an early age. This method was ...
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750 million genetically modified mosquitoes soon released in the wild!

explica,  explica,  2020.
Rather frightening mosquitoes will be released on an archipelago in Florida. The goal? Reduce the population of their more dangerous congeners who can transmit certain diseases.
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750 million genetically modified mosquitoes to be released across Florida Keys

A. Zahid,  Sky News,  2020.
Authorities have approved plans for genetically modified mosquitoes to be released across the Florida Keys from next year. British-based firm Oxitec has designed the project to test whether the altered mosquitoes are a viable alternative to pesticides to control and prevent the ...
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750 Million GM Mosquitoes Will Be Released in the Florida Keys

L. Winter,  The Scientist,  2020.
With the aim of reducing rates of the mosquito-borne illnesses yellow fever and dengue, a pilot program will release 750 million genetically modified mosquitoes into the Florida Keys in 2021, thanks to approval by the barrier islands’ Mosquito Control District Board of ...
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750 million GM mosquitos set for release in Florida Keys.

Editorial Staff,  E&T,  2020.
Local authorities have approved proposals to release hundreds of millions of genetically modified (GM) modified mosquitos in Florida, in an effort to control populations of diseases spread by the organism.
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A Billion Lab-Grown Mosquitos Are Being Released and People Are Freaking Out

V. Kipnis,  Vice,  2021.
On Thursday afternoon, six Florida Keys residents will walk out into their yards and set out aquamarine cardboard boxes filled with mosquito larvae. Then, water will be poured into the containers. For the next ten days, as the mosquitoes grow into adults, their neighbors will be ...
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A CRISPR endonuclease gene drive reveals two distinct mechanisms of inheritance bias

S. A. N. Verkuijl, E. González, J. X. D. Ang, M. Li, N. P. Kandul, M. Anderson, O. S. Akbari, M. Bonsall and L. Alphey,  bioRxiv,  2020.12.15.421271. 2020.
In this study, we report the functioning of sds3, bgcn, and nup50 expressed Cas9 in an Aedes aegypti homing split drive system targeting the white gene. We report their inheritance biasing capability, propensity for maternal deposition, and zygotic/somatic expression. ...
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A wAlbB Wolbachia&lt transinfection displays stable phenotypic effects across divergent Aedes aegypti mosquito backgrounds

P. A. Ross, X. Gu, K. L. Robinson, Q. Yang, E. Cottingham, Y. Zhang, H. L. Yeap, X. Xu, N. M. Endersby-Harshman and A. A. Hoffmann,  bioRxiv,  2021.06.25.450002. 2021.
Aedes mosquitoes harboring intracellular Wolbachia bacteria are being released in arbovirus and mosquito control programs. With releases taking place around the world, understanding the contribution of host variation to Wolbachia phenotype is crucial. We generated a Wolbachia ...
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Advances in vector control science: Rear-and-release strategies show promise… but don’t forget the basics

Ritchie, SAJ, B. J.,  Journal of Infectious Diseases,  215:S103-S108. 2017.
Both chikungunya and Zika viruses have recently swept from Africa across the Pacific to the Americas, causing major outbreaks of disease in humans. In the meantime, dengue epidemics continue throughout the tropics. Traditional vector control programs based on strategies from ...
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An accident waiting to happen: Tech company to release 750 MILLION GMO mosquitoes in Florida to fight dengue fever

Z. Sky,  NEWSTARGET,  2020.
Oxitec plans to release 750 million OX5034s into the Florida Keys, something that sounds like the beginning of a doomed science-fiction movie. But the most shocking thing here is the fact that Oxitec received the EPA’s approval in May.
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Artificial Selection Finds New Hypotheses for the Mechanism of Wolbachia-Mediated Dengue Blocking in Mosquitoes

S. A. Ford, I. Albert, S. L. Allen, S. F. Chenoweth, M. Jones, C. Koh, A. Sebastian, L. T. Sigle and E. A. McGraw,  Frontiers in Microbiology,  11:1456. 2020.
We recently used experimental evolution to reveal that Wolbachia-mediated dengue blocking could be selected upon in the A. aegypti host and showed evidence that strong levels of blocking could be maintained by natural selection. In this study, we investigate the genetic variation ...
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Assessment of a Novel Adult Mass-Rearing Cage for Aedes albopictus (Skuse) and Anopheles arabiensis (Patton).

H. Maïga, W. Mamai, N. S. Bimbilé Somda, T. Wallner, B. S. Poda, G. Salvador-Herranz, R. Argiles-Herrero, H. Yamada and J. Bouyer,  Insects,  11:801. 2020.
Successful implementation of the sterile insect technique (SIT) against Aedes albopictus and Anopheles arabiensis relies on a continuous supply of sterile males. To meet this requirement, optimization of the mass-rearing techniques is needed. This study, therefore, aims to assess ...
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Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release

N. Pocquet, O. O’Connor, H. A. Flores, J. Tutagata, M. Pol, D. J. Hooker, C. Inizan, S. Russet, J. M. Duyvestyn, E. C. Pacidônio, D. Girault, D. da Silva Gonçalves, M. Minier, F. Touzain, E. Chalus, K. Lucien, F. Cheilan, T. Derycke, S. Laumond, C. P. Sim,  PLOS Neglected Tropical Diseases,  15:e0009752. 2021.
Dengue represents a risk for almost half of the world’s population, especially throughout the tropics. In New Caledonia, dengue outbreaks have become more frequent in the past decade along with the recent circulation of chikungunya and Zika viruses. The opportunity to use the ...
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Australian research takes aim at dengue, another killer virus

E. Connors,  Finanacial Review,  2020.
Australian researchers have teamed up with Indonesian philanthropists to strike a blow against dengue fever, the deadly disease that was a growing scourge in south-east Asia and South America long before COVID-19.
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Australian scientists slash dengue fever in Indonesia by infecting mosquitoes with bacteria

A. Barker,  ABC News,  2020.
Australian scientists may have found the secret to eradicating dengue fever, with a lengthy trial in Indonesia drastically reducing the incidence of the mosquito-borne virus.
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Bacteria-Laced Mosquitoes Limit Spread of Dengue

A. Heidt,  The Scientist,  2020.
Researchers have infected Aedes aegypti mosquitoes—the species responsible for passing on many diseases—with bacteria called Wolbachia with the intent of reducing the insects’ ability to pass on dengue to people.
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Bill Gates finances the creation of transgenic mosquitoes

Explica.co,  explica,  2021.
Male mosquitoes (they do not bite. They feed on nectar, not blood) from Oxitec will emerge from release boxes placed in six locations in the Florida Keys to mate with local female mosquitoes (they do bite. They do feed on blood). The female offspring from these encounters cannot ...
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Bill Gates-backed startup releases millions of genetically modified mosquitoes

ENTREPRENEUR STAFF,  Entrepreneur,  2021.
The British company Oxitec released a cloud of hundreds of millions of genetically modified mosquitoes to study how to control their reproduction and thus stop the spread of dengue, Zika, malaria, etc. As reported by Axios , the company, which is funded by the Bill and Melinda ...
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Bug board OKs release of genetically modified mosquitoes

T. O'Hara,  keynews.com,  2020.
After nearly 10 years of debate, the Florida Keys Mosquito Control District board has approved an agreement with biotech company Oxitec to conduct a test release of genetically modified mosquitoes in Monroe County as part of a mosquito limiting or eradication plan.
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BUZZ OFF Florida residents blast pest control ‘TERRORISTS’ over plans to unleash a BILLION mutant mosquitoes in the Keys

J. Bentley-York,  The SUN,  2021.
The areas Mosquito Control District (FKMCD) are set to release the gene hacked ‘fraken-squitoes' in a bid to fight insect-borne viruses like yellow fever. A BILLION mutant mosquitoes are set to be released in the Keys to fight insect-borne viruses like yellow fever Florida pest ...
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Coalition Against GMO Mosquito Condemns Release of Genetically Engineered Mosquitoes

GMO Free USA,  3BL CSRwire,  2021.
Despite a decade of community opposition, the experimental release of genetically engineered mosquitoes begins today in the Florida Keys. British biotech corporation, Oxitec, in collaboration with the Florida Keys Mosquito Control District, plans to release up to a billion ...
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Converting female mosquitoes to non-biting males with implications for mosquito control

M. V. Candy,  Vet Candy,  2020.
Virginia Tech researchers have proven that a single gene can convert female Aedes aegypti mosquitoes into fertile male mosquitoes and identified a gene needed for male mosquito flight. Male mosquitoes do not bite and are unable to transmit pathogens to humans. Female mosquitoes, ...
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CRISPR/Cas9 knockout of female-biased genes AeAct-4 or myo-fem in Ae. aegypti results in a flightless phenotype in female, but not male mosquitoes

S. O’Leary and Z. N. Adelman,  PLOS Neglected Tropical Diseases,  14:e0008971. 2020.
Author summary Ae. aegypti is the most important vector of arboviruses throughout the world, and new strategies are urgently needed to add to our existing arsenal of control methods to prevent or halt disease transmission. Importantly, only female Ae. aegypti mosquitoes transmit ...
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Current Effector and Gene-Drive Developments to Engineer Arbovirus-Resistant Aedes aegypti (Diptera: Culicidae) for a Sustainable Population Replacement Strategy in the Field

W. R. Reid, K. E. Olson and A. W. E. Franz,  J Med Entomol,  2021.
Conventional mosquito control efforts based on insecticide treatments and/or the use of bednets and window curtains are currently insufficient to reduce arbovirus prevalence in affected regions. Novel, genetic strategies that are being developed involve the genetic manipulation ...
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Cytoplasmic incompatibility: an autocidal mechanism for mosquito population control

V. Dev,  BugBitten BMC,  2020.
Cytoplasmic incompatibility resulting in non-reciprocal fertility is a naturally occurring phenomenon, but remains unexplored to greater extent for the control of insect vector populations. This mechanism deserves priority for mosquito control and reducing disease transmission, ...
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Deep dive: Florida’s GM mosquito experiment aims to rewrite rules of vector-borne diseases

S. Kannan,  India Today,  2020.
A pathbreaking bioengineering experiment on mosquito populations that could have massive implications for tropical malaria-affected countries like India has got underway in Florida, US.
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Editorial Expression of Concern: Transgenic Aedes aegypti Mosquitoes Transfer Genes into a Natural Population

B. R. Evans, P. Kotsakiozi, A. L. Costa-Da-Silva, R. S. Ioshino, L. Garziera, M. C. Pedrosa, A. Malavasi, J. F. Virginio, M. L. Capurro and J. R. Powell,  Scientific Reports,  10:2. 2020.
Shortly after publication of this Article in September 2019, the Editors were alerted to concerns regarding the interpretation of the data and some of the conclusions.
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Eliminating Mosquitoes with Precision Guided Sterile Males

M. Li, T. Yang, M. Bui, S. Gamez, T. Wise, N. P. Kandul, J. Liu, L. Alcantara, H. Lee, J. R. Edula, R. Raban, Y. Zhan, Y. Wang, N. DeBeaubien, J. Chen, H. M. Sanchez C, J. B. Bennett, I. Antoshechkin, C. Montell, J. M. Marshall and O. S. Akbari,  bioRxiv,  2021.03.05.434167. 2021.
Here we develop a molecular genetic control system termed precision guided sterile insect technique (pgSIT) in Aedes aegypti. PgSIT uses a simple CRISPR-based approach to generate sterile males that are deployable at any life stage. Supported by mathematical models, we ...
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Engineered action at a distance: Blood-meal-inducible paralysis in Aedes aegypti

R. E. Haghighat-Khah, T. Harvey-Samuel, S. Basu, O. StJohn, S. Scaife, S. Verkuijl, E. Lovett and L. Alphey,  PLOS Neglected Tropical Diseases,  13:e0007579. 2019.
Here we demonstrate that it is possible to engineer ‘non-cell autonomous’ effectors–that is where the effect (e.g. the action of a toxic protein) can act on cells distant from the tissues in which they are originally expressed. To achieve this we utilised the endogenous ...
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Engineered resistance to Zika virus in transgenic Aedes aegypti expressing a polycistronic cluster of synthetic small RNAs

Buchman, AG, S.; Li, M.; Antoshechkin, I.; Li, H. H.; Wang, H. W.; Chen, C. H.; Klein, M. J.; Duchemin, J. B.; Paradkar, P. N.; Akbari, O. S.,  Proceedings of the National Academy of Sciences of the United States of America,  116:3656-3661. 2019.
Recent Zika virus (ZIKV) outbreaks have highlighted the necessity for development of novel vector control strategies to combat arboviral transmission, including genetic versions of the sterile insect technique, artificial infection with Wolbachia to reduce population size and/or ...
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Enhancement of Aedes aegypti susceptibility to dengue by Wolbachia is not supported

T. H. Ant, M.-V. Mancini, J. Martinez and S. P. Sinkins,  Nature Communications,  11:6111. 2020.
King et al.3 used DENV infection and transmission modelling to reinterpret experimental data from two previous studies4,5. The authors claimed that wMel Wolbachia increase the mean susceptibility of Ae. aegypti to DENV, contradicting various other studies6,7,8,9,10,11,12. Here, ...
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EPA Approves Experimental Use Permit to Test Innovative Biopesticide Tool to Better Protect Public Health

EPA,  EPA,  2020.
Today, after extensive evaluation of the best available science and public input, the U.S. Environmental Protection Agency (EPA) has granted an experimental use permit (EUP) to Oxitec Ltd. to field test the use of genetically engineered Aedes aegypti mosquitoes as a way to reduce ...
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EPA approves field trials of genetically modified mosquito

A. Wernick,  WBFO npr,  2020.
The Environmental Protection Agency has given biotech company Oxitec the go-ahead to test the effectiveness of genetically modified mosquitoes in parts of Florida and Texas. Oxitec has been developing genetically modified mosquitoes in hopes of reducing local populations of ...
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EPA faces suit over plan to release genetically engineered mosquitoes

R. Frazin,  The Hill,  2020.
The Environmental Protection Agency (EPA) is facing a lawsuit over its approval of a plan to release genetically modified mosquitoes in Florida and Texas
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EPA Grants First Permit to Test Genetically Modified Mosquitoes

Adam Allington,  Bloomberg Law,  2020.
British biotech company Oxitec Ltd was granted an experimental use permit to release a genetically engineered type of the mosquito species Aedes aegypti, which is a known vector of Zika virus and viruses that cause yellow fever and dengue fever, the Environmental Protection ...
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EPA Seeks Public Comment on Proposed Amendment to Experimental Use Permit for Genetically Engineered Mosquitoes

PCT Staff,  Pest Control Technology,  2021.
EPA is seeking public comment on a proposed amendment to extend and expand an approved Experimental Use Permit (EUP). The EUP currently allows Oxitec Ltd. to field test the use of genetically engineered Aedes aegypti mosquitoes as a way to reduce mosquito populations in Florida ...
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Establishment of Wolbachia Strain wAlbB in Malaysian Populations of Aedes aegypti for Dengue Control

W. A. Nazni, A. A. Hoffmann, A. NoorAfizah, Y. L. Cheong, M. V. Mancini, N. Golding, G. M. R. Kamarul, M. A. K. Arif, H. Thohir, H. NurSyamimi, M. Z. ZatilAqmar, M. NurRuqqayah, A. NorSyazwani, A. Faiz, F.-R. M. N. Irfan, S. Rubaaini, N. Nuradila, N. M. N,  Current Biology,  29:4241-4248.e5. 2019.
Dengue has enormous health impacts globally. A novel approach to decrease dengue incidence involves the introduction of Wolbachia endosymbionts that block dengue virus transmission into populations of the primary vector mosquito, Aedes aegypti. The wMel Wolbachia strain has ...
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Fear of Oxitec mosquito release grows

T. Java,  Florida Keys Free Press,  2020.
A local effort has emerged to exclude Key Largo from a test release of genetically modified Aedes aegypti mosquitoes planned for the spring. The U.S. Environmental Protection Agency and the Florida Keys Mosquito Control District have approved a release of United Kingdom-based ...
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Field performance of sterile male mosquitoes released from an uncrewed aerial vehicle

J. Bouyer, N. J. Culbert, A. H. Dicko, M. G. Pacheco, J. Virginio, M. C. Pedrosa, L. Garziera, A. T. M. Pinto, A. Klaptocz, J. Germann, T. Wallner, G. Salvador-Herranz, R. A. Herrero, H. Yamada, F. Balestrino and M. J. B. Vreysen,  Science Robotics,  5:10. 2020.
Genetic control methods of mosquito vectors of malaria, dengue, yellow fever, and Zika are becoming increasingly popular due to the limitations of other techniques such as the use of insecticides. The sterile insect technique is an effective genetic control method to manage ...
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Fighting disease: How are genetically engineered mosquitoes regulated?

A. Julie,  Global News,  2021.