Keywords: mosquitoes

Symbiotic Interactions Between Mosquitoes and Mosquito Viruses

M. Altinli, E. Schnettler and M. Sicard,  Front Cell Infect Microbiol,  11:694020. 2021.
Mosquitoes not only transmit human and veterinary pathogens called arboviruses (arthropod-borne viruses) but also harbor mosquito-associated insect-specific viruses (mosquito viruses) that cannot infect vertebrates. In the past, studies investigating mosquito viruses mainly ...
Keywords: , , ,

Predicting the spread and persistence of genetically modified dominant sterile male mosquitoes

A. Ickowicz, S. D. Foster, G. R. Hosack and K. R. Hayes,  Parasites and Vectors,  14:480. 2021.
Reproductive containment provides an opportunity to implement a staged-release strategy for genetic control of malaria vectors, in particular allowing predictions about the spread and persistence of (self-limiting) sterile and male-biased strains to be compared to outcomes before ...
Keywords: , , ,

The Complex Lives of Mosquitoes: The Key for Malaria Control

F. Okumu,  ISGlobal,  2021.
Mosquitoes spread diseases to millions of people around the world, yet they remain poorly understood by most. Studying their biology and behaviours can help us combat, and eventually eliminate, dangerous diseases such as malaria and dengue fever.There are nearly 3,500 species of ...
Keywords: , , ,

New mosquito control tools are critical

L. Braack,  Open Access Government,  2021.
Globally, we are making slow headway in the fight against malaria, but there has been progress, nonetheless. Since 2000, 39 countries and territories have managed to rid themselves of malaria; the most recent is China. Existing tools can achieve local elimination, but the battle ...
Keywords: , , ,

Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus

C. Lutrat, R. P. Olmo, T. Baldet, J. Bouyer and E. Marois,  bioRxiv,  2021.07.28.454191. 2021.
Aedes albopictus is a major vector of arboviruses. Better understanding of its sex determination is crucial for developing mosquito control tools, especially genetic sexing strains. In Aedes aegypti, Nix is the primary gene responsible for masculinization and Nix-expressing ...
Keywords: , , ,

Sex separation of Aedes spp. mosquitoes for sterile insect technique application: a review

B. M. Moran-Aceves, C. F. Marina, A. Dor, P. Liedo and J. Toledo,  Entomologia Experimentalis Et Applicata,  10. 2021.
Separation of the sexes is necessary for the application of the sterile insect technique (SIT) in mosquitoes due to the hematophagous habits and disease vector activity of the females. In this review we analyze the history, current status, and future perspectives for the ...
Keywords: , , ,

Wolbachia as translational science: controlling mosquito-borne pathogens

E. P. Caragata, H. L. C. Dutra, P. H. F. Sucupira, A. G. A. Ferreira and L. A. Moreira,  Trends in Parasitology,  2021.
In this review we examine how exploiting the Wolbachia?mosquito relationship has become an increasingly popular strategy for controlling arbovirus transmission. Field deployments of Wolbachia-infected mosquitoes have led to significant decreases in dengue virus incidence via high ...
Keywords: , , ,

Combating mosquito-borne diseases using genetic control technologies

G.-H. Wang, S. Gamez, R. R. Raban, J. M. Marshall, L. Alphey, M. Li, J. L. Rasgon and O. S. Akbari,  Nature Communications,  12:4388. 2021.
Mosquito-borne diseases, such as dengue and malaria, pose significant global health burdens. Unfortunately, current control methods based on insecticides and environmental maintenance have fallen short of eliminating the disease burden. Scalable, deployable, genetic-based ...
Keywords: , , ,

The (Losing) Battle Against Mosquitoes In Texas

J. Clayton,  Texas Public Radio,  2021.
Jerry Clayton: Mosquitoes are a fact of life in Texas, and the battle against the pesky biting insects is never ending. But there are some new weapons on the horizon. Zach Adleman is an associate professor of entomology at Texas A&M University. He joins us today. Thanks for being ...
Keywords: , , ,

Sterilizing skeeters using CRISPR/Cas9

H. Tasoff,  Phy Org,  2021.
Mosquitoes are one of humanity's greatest nemeses, estimated to spread infections to nearly 700 million people per year and cause more than one million deaths. UC Santa Barbara Distinguished Professor Craig Montell has made a breakthrough in one technique for controlling ...
Keywords: , , ,

Malaria-Resistant Mosquitoes (Diptera: Culicidae); The Principle is Proven, But Will the Effectors Be Effective?

Z. N. Adelman and B. B. Kojin,  Journal of Medical Entomology,  58:1997-2005. 2021.
Over the last few decades, a substantial number of anti-malarial effector genes have been evaluated for their ability to block parasite infection in the mosquito vector. While many of these approaches have yielded significant effects on either parasite intensity or prevalence of ...
Keywords: , , ,

Next gen insect control

E. Unglesbee,  Progressive Farmer,  2021.
Dubbed "self-limiting" insects by their makers, a UK-based biotechnology company called Oxitec, these insects are genetically modified (GM) with an inserted gene that permits only male offspring to survive. Once released into a pest community, the GM insects gradually lower the ...
Keywords: , , ,

Evidence for natural hybridization and novel Wolbachia strain superinfections in the Anopheles gambiae complex from Guinea

C. L. Jeffries, C. Cansado-Utrilla, A. H. Beavogui, C. Stica, E. K. Lama, M. Kristan, S. R. Irish and T. Walker,  Royal Society Open Science,  8:18. 2021.
Wolbachia, a widespread bacterium which can influence mosquito-borne pathogen transmission, has recently been detected within Anopheles (An.) species that are malaria vectors in Sub-Saharan Africa. Although studies have reported Wolbachia strains in the An. gambiae complex, ...
Keywords: , , ,

When More is Less: Mosquito Population Suppression Using Sterile, Incompatible and Genetically Modified Male Mosquitoes

S. L. Dobson,  Journal of Medical Entomology,  58:1980-1986. 2021.
The current review of the Sterile Insect Technique (SIT) is motivated by new technologies and the recent renaissance of male release field trials, which is driving an evolution in mosquito control and regulation. Practitioners that are releasing male mosquitoes would do well to ...
Keywords: , , ,

Sterile Insect Technique: Lessons From the Past

M. Q. Benedict,  Journal of Medical Entomology,  58:1974-1979. 2021.
When E.F. Knipling conceived of the release of sexually sterile insects to suppress wild populations, he laid down several fundamental qualities that characterized suitable target species—some of which mosquitoes generally violate—including high reproductive rates and large ...
Keywords: , , ,

Mutant mosquitoes: GM insects ‘engineered’ in ‘new approach to pest control’

T. Fish,  EXPRESS,  2020.
This cutting-edge research provides the foundations for plans to prevent genetically modified organisms from reproducing with wild organisms.
Keywords: , , ,

GeneConvene Global Collaborative Webinar Series

David O'Brochta and Hector Quemada,  GeneConvene Global Collaborative,  2020.
This is a series of public scientific and technical seminars by researchers actively involved in the research and development of engineered gene drive and related systems. Presentations will be aimed at other researchers and scientists, highlighting the latest investigations in ...
Keywords: , , ,

Genome Editing 2020: Ethics and Human Rights in Germline Editing in Humans and Gene Drives in Mosquitoes

G. J. Annas,  American Journal of Law and Medicine,  46:143-165. 2020.
G. J. Annas (2020). American Journal of Law and Medicine. doi: 10.1177/0098858820933492. I begin with a discussion of so far disastrously unsuccessful attempts to regulate germline editing in humans, including a summary of the first application of germline genome editing in ...
Keywords: , , ,

The need for new vector control approaches targeting outdoor biting anopheline malaria vector communities

S. Sougoufara, E. C. Ottih and F. Tripet,  Parasites & Vectors,  13:15. 2020.
Since the implementation of Roll Back Malaria, the widespread use of insecticide-treated nets (ITNs) and indoor residual spraying (IRS) is thought to have played a major part in the decrease in mortality and morbidity achieved in malaria-endemic regions. In the past decade, ...
Keywords: , , ,

Modeling confinement and reversibility of threshold-dependent gene drive systems in spatially-explicit Aedes aegypti populations

H. M. Sánchez C, J. B. Bennett, S. L. Wu, G. Rašić, O. S. Akbari and J. M. Marshall,  BMC Biology,  18:50. 2020.
Here, we model hypothetical releases of two recently engineered threshold-dependent gene drive systems—reciprocal chromosomal translocations and a form of toxin-antidote-based underdominance known as UDMEL—to explore their ability to be confined and remediated.
Keywords: , , ,

Guidance Framework for Testing the Sterile Insect Technique as a Vector Control Tool against Aedes-Borne Diseases

WHO & IAEA,  WHO & IAEA,  2020.
This document is intended to be a comprehensive guide for programme managers tasked with recommending a “go/no-go” decision on testing, full deployment and scale-up of the sterile insect technique (SIT) in regions of the world affected by diseases transmitted by Aedes ...
Keywords: , , ,

Opinions of key stakeholders on alternative interventions for malaria control and elimination in Tanzania

M. F. Finda, N. Christofides, J. Lezaun, B. Tarimo, P. Chaki, A. H. Kelly, N. Kapologwe, P. Kazyoba, B. Emidi and F. O. Okumu,  Malaria Journal,  19:164. 2020.
Malaria control in Tanzania currently relies primarily on long-lasting insecticidal nets and indoor residual spraying, alongside effective case management and behaviour change communication. This study explored opinions of key stakeholders on the national progress towards malaria ...
Keywords: , , ,

Can we kill the dreaded mosquito? Do we even want to?

Stacey McKenna,  Sierra,  2020.
As a major vector for disease, the mosquito has harmed more human beings than just about any other animal, and a changing climate is only boosting those numbers. As the range of disease-carrying species of mosquitoes expands, so does their ability to transmit the parasites and ...
Keywords: , , ,

‘Gene Drive’ to curb malaria raises ethical questions as well

Gyanedra Nath Mitra,  The Pioneer,  2020.
A new technology ‘Gene Drive’ for mosquito control is currently confined to the laboratory since it raises an ethical question, if such a technology could in future be misused to the detriment of humanity.
Keywords: , , ,

Should the humans be allowed to genetically modify insects?

Olivia Abbe,  NYK Daily,  2020.
Genetic engineering, also called Genetic modification or Genetic manipulation, is the direct manipulation of an organism’s genes using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across ...
Keywords: , , ,

Public health concerns over gene-drive mosquitoes: will future use of gene-drive snails for schistosomiasis control gain increased level of community acceptance?

D. O. Famakinde,  Pathogens and Global Health,  2020.
With the advent of CRISPR (clustered regularly interspaced short palindromic repeat)-based gene drive, present genetic research in schistosomiasis vector control envisages the breeding and release of transgenic schistosome-resistant (TSR) snail vectors to curb the spread of the ...
Keywords: , , ,

The Buzz About Genetically Modified Mosquitoes – a podcast

The Scientist Creative Services Team,  The Scientist,  podcast. 2020.
Mosquito-borne diseases afflict a large portion of the world. In this month’s episode, we consider genetic methods to eradicate diseases such as Zika fever, Dengue fever, and malaria. We spoke with Omar Akbari, professor of Cell and Developmental Biology at the University of ...
Keywords: , , ,

Genetic Control of Mosquitoes

Alphey, L.,  Annual Review of Entomology,  59:205-224. 2019.
Genetics can potentially provide new, species-specific, environmentally friendly methods for mosquito control. Genetic control strategies aim either to suppress target populations or to introduce a harm-reducing novel trait. Different approaches differ considerably in their ...
Keywords: , , ,

A Controversial Swarm Of Genetically Modified Mosquitoes In A Lab In Italy

NPR,  ,  2019.
An international team of scientists is conducting a controversial experiment in Italy. The experiment is designed to test genetically modified mosquitoes that researchers hope could provide a powerful new weapon to fight malaria, which remains one of the world's greatest ...
Keywords: , , ,

Gene drive mosquitoes and the new era of medical colonialism

Mayet, MC, L. I.; Sirinathsingji, E,  GM Watch,  2019.
The highly contentious issue of gene drive technologies – a novel extreme form of genetic engineering designed to alter or even eradicate entire populations and species – was at the heart of the international negotiations at the biennial UN Biodiversity Conference held in ...
Keywords: , , ,

Male competition and the evolution of mating and ire-history traits in experimental populations of Aedes aegypti

Qureshi, AA, A.; Hollis, B.; Ponlawat, A.; Cator, L. J.,  Proceedings of the Royal Society B-Biological Sciences,  286:20190591. 2019.
Aedes aegypti is an important disease vector and a major target of reproductive control efforts. We manipulated the opportunity for sexual selection in populations of Ae. aegypti by controlling the number of males competing for a single female. Populations exposed to higher ...
Keywords: , , ,

Population genetics of Anopheles funestus, the African malaria vector, Kenya

Ogola, EOO, J. O.; Mwangangi, J. M.; Masiga, D. K.; Tchouassi, D. P.,  Parasites & Vectors,  12:15. 2019.
Anopheles funestus is among the major malaria vectors in Kenya and sub-Saharan Africa and has been recently implicated in persistent malaria transmission. However, its ecology and genetic diversity remain poorly understood in Kenya.MethodsUsing 16 microsatellite loci, we examined ...
Keywords: , , ,

CRISPR in Parasitology: Not Exactly Cut and Dried!

Bryant, JMB, S.; Glover, L.; Hutchinson, S.; Rachidi, N.,  Trends in Parasitology,  35:409-422. 2019.
CRISPR/Cas9 technology has been developing rapidly in the field of parasitology, allowing for the dissection of molecular processes with unprecedented efficiency. Optimization and implementation of a new technology like CRISPR, especially in nonmodel organisms, requires ...
Keywords: , , ,

Modelling the potential of genetic control of malaria mosquitoes at national scale

North, ARB, Austin; Godfray, H. Charles J.,  BMC Biology,  17:26. 2019.
The persistence of malaria in large parts of sub-Saharan Africa has motivated the development of novel tools to complement existing control programmes, including gene-drive technologies to modify mosquito vector populations. Here, we use a stochastic simulation model to explore ...
Keywords: , , ,

A Question of Consent: Exterminator Mosquitoes in Burkina Faso

ETC group,  ,  2019.
Target Malaria’s planned release of GMO mosquitos is step toward release of gene drive mosquitoes, a high-risk technology aimed at the elimination of entire species. Hundreds of organizations have demanded a moratorium on the use of this technology outside of ...
Keywords: , , ,

Large-cage assessment of a transgenic sex-ratio distortion strain on populations of an African malaria vector

Facchinelli, LN, A.; Collins, C.; Menichelli, M.; Persampieri, T.; Bucci, A.; Spaccapelo, R.; Crisanti, A.; Benedict, M.,  Parasites & Vectors,  12:70. 2019.
Novel transgenic mosquito control methods require progressively more realistic evaluation. The goal of this study was to determine the effect of a transgene that causes a male-bias sex ratio on Anopheles gambiae target populations in large insectary cages. Life history ...
Keywords: , , ,

The Release of Genetically Engineered Mosquitoes in Burkina Faso: Bioeconomy of Science, Public Engagement and Trust in Medicine

Beisel, UG, J. K.,  African Studies Review,  62:164-173. 2019.
Malaria, which is transmitted by mosquitoes, continues to be responsible for a significant number of disease episodes and childhood deaths on the African continent. A variety of mosquito control strategies are currently inplace, but since case numbers are rising again, and drug ...
Keywords: , , ,

Gene drive for population genetic control: non-functional resistance and parental effects

Beaghton, AKH, Andrew; Nolan, Tony; Crisanti, Andrea; Burt, Austin,  Proceedings of the Royal Society B: Biological Sciences,  286:20191586. 2019.
Gene drive is a natural process of biased inheritance that, in principle, could be used to control pest and vector populations. As with any form of pest control, attention should be paid to the possibility of resistance evolving. For nuclease-based gene drive aimed at suppressing ...
Keywords: , , ,

Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus

Liu, TY, W. Q.; Xie, Y. G.; Liu, P. W.; Xie, L. H.; Lin, F.; Li, C. Y.; Gu, J. B.; Wu, K.; Yan, G. Y.; Chen, X. G.,  Insect Science,  26:1045-1054. 2019.
Aedes (Stegomyia) albopictus, also known as the Asian tiger mosquito, is a mosquito which originated in Asia. In recent years, it has become increasingly rampant throughout the world. This mosquito can transmit several arboviruses, including dengue, Zika and chikungunya viruses, ...
Keywords: , , ,

Alternative strategies for mosquito-borne arbovirus control

Achee, NLG, J. P.; Vatandoost, H.; Seixas, G.; Pinto, J.; Ching-Ng, L.; Martins, A. J.; Juntarajumnong, W.; Corbel, V.; Gouagna, C.; David, J. P.; Logan, J. G.; Orsborne, J.; Marois, E.; Devine, G. J.; Vontas, J.,  PLOS Neglected Tropical Diseases,  13:e0006822. 2019.
Mosquito-borne virusessuch as Zika, chikungunya, dengue fever, and yellow fever, among othersare of global importance. Although vaccine development for prevention of mosquito-borne arbovirus infections has been a focus, mitigation strategies continue to rely on vector control. ...
Keywords: , , ,

CRISPR Gene Drive (Complete guide 2019)

Every Cell A Universe,  ,  2018.
Crispr gene drive - malaria cure and a new way to look at conservation.
Keywords: , , ,

Why is this African village letting mosquitoes in?

BBC,  BBC,  2018.
This is a BBC spot about the first release of genetically modified mosquitoes in Africa by the Target Malaria team. Very little information about gene drive but the context of this spot is notable.
Keywords: , , ,

Gene drive used to turn all fremale mosquitos sterile

Timmer, J,  ARS Technica,  2018.
We've known for a long time that we can limit malaria infections by controlling the mosquitos that transmit them. But that knowledge hasn't translated into control efforts that have always been completely successful. Many of the approaches we've used to control mosquitos have ...
Keywords: , , ,

Malaria mosquitoes wiped out in lab trials of gene drive testing

Kelland, K,  The Wire,  2018.
London: Scientists have succeeded in wiping out a population of caged mosquitoes in laboratory experiments using a type of genetic engineering known as a gene drive, which spread a modification blocking female reproduction.; ; The researchers, whose work was published on Monday ...
Keywords: , , ,

Gene drives breakthrough needs urgent restraint

Steinbrecher, R,  GMWatch,  2018.
Gene drive researchers associated with Target Malaria and funded by US DARPA, the GATES Foundation and the UK BBSRC have just managed to crash a population of caged mosquitoes after 7-11 generations.(1); ; This is a first, and it has brought this technology beyond the proof of ...
Keywords: , , ,

Here’s the Plan to End Malaria With Crispr-Edited Mosquitoes

Molteni, M,  Wired,  2018.
In 2003, scientists at London’s Imperial College hatched a somewhat out-there idea. They wanted to deal with the increasingly pesticide-resistant mosquitoes that were killing half a million people a year by spreading malaria in sub-Saharan Africa. What biologists Austin Burt ...
Keywords: , , ,

For the first time, researchers will release genetically engineered mosquitoes in Africa

Ike Swetitz,  STAT,  2018.
The government of Burkina Faso granted scientists permission to release genetically engineered mosquitoes anytime this year or next, researchers announced Wednesday. It’s a key step in the broader efforts to use bioengineering to eliminate malaria in the region.
Keywords: , , ,

Gene drive to reduce malaria transmission in sub-Saharan Africa

A. Burt, M. Coulibaly, A. Crisanti, A. Diabate and J. K. Kayondo,  Journal of Responsible Innovation,  5:S66-S80. 2018.
Gene drive is a natural process by which some genes are inherited at a greater-than-Mendelian rate and can spread through a population even if they cause harm to the organisms carrying them. Many different synthetic gene drive systems have been proposed to suppress the number of ...
Keywords: , , ,

Population seasonality and release timing significantly affect the probability of establishment for small releases of gene drive mosquitoes

Nikolov, MO, A. L.; Beaghton, A. K.; Beaghton, P. J.; Wenger, E. A.; Burt, A.; Welkhoff, P. A.,  American Journal of Tropical Medicine and Hygiene,  99:367-367. 2018.
Highly efficient CRISPR/Cas9 gene-drive systems have recently been developed, targeting reproductive-capacity and malaria-competency loci of malaria transmitting vector species, such as An. gambiae. The resulting drive systems aim to either suppress the local wild-type population ...
Keywords: , , ,

A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes

Kyrou, KH, Andrew M.; Galizi, Roberto; Kranjc, Nace; Burt, Austin; Beaghton, Andrea K.; Nolan, Tony; Crisanti, Andrea,  Nature Biotechnology,  36:1062–1066. 2018.
In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control differentiation of the two sexes. The female transcript, unlike the male, contains an exon (exon 5) ...
Keywords: , , ,

Gene drive for Malaria control | Andrea Crisanti |

TEDx,  ,  2017.
Andrea discusses his team's laboratory work that has developed a revolutionary technology to spread genetic modifications from few laboratory mosquitoes to wild populations to eradicate malaria in the near future
Keywords: , , ,

Driving out malaria

Nolan, TC, A.,  Scientist,  2017.
In recent years, researchers have sequenced the genomes of several Anopheles mosquito species, including those responsible for nearly all of the malaria transmission in Africa. With this information, they have begun to identify the genes underlying the insects’ ability to ...
Keywords: , , ,

Using Gene Drive to Control Malaria

The Scientist,  The Scientist,  2016.
This article provides illustrations for how gene drive works, how gene drives spread, and how gene drive could be used to control malaria using population-wide gene knockout, skewed sex ratio, and population-wide gene knock-in techniques. Visit the article to view all ...
Keywords: , , ,

Gene silencing and gene drive in dengue vector control

Paulraj, MGI, S.; Reegan, A. D.,  Indian Journal of Natural Products and Resources,  7:193-200. 2016.
Vector-borne diseases are the most feared diseases throughout the world. Mosquitoes are the prime human disease vectors as they are responsible for nearly one million human deaths every year. So they are declared as the most dangerous insects to mankind. Aedes aegypti and Ae. ...
Keywords: , , ,

Genetic Control of Mosquitoes.

Alphey, L.,  Annual Review of Entomology,  59:205-224. 2014.
Genetics can potentially provide new, species-specific, environmentally friendly methods for mosquito control. Genetic control strategies aim either to suppress target populations or to introduce a harm-reducing novel trait. Different approaches differ considerably in their ...
Keywords: , , ,

A synthetic homing endonuclease-based gene drive system in the human malaria mosquito

Windbichler, NM, M.; Papathanos, P. A.; Thyme, S. B.; Li, H.; Ulge, U. Y.; Hovde, B. T.; Baker, D.; Monnat, R. J.; Burt, A.; Crisanti, A.,  Nature,  473:212-215. 2011.
Genetic methods of manipulating or eradicating disease vector populations have long been discussed as an attractive alternative to existing control measures because of their potential advantages in terms of effectiveness and species specificity(1-3). The development of ...
Keywords: , , ,

Deployment of innovative genetic vector control strategies: progress on regulatory and biosafety aspects, capacity building and development of best-practice guidance

Beech, CV, S.S.; Quinlan, M.M.; Capurro, Margareth L.; Alphey, L.; Bayard, V.; Bouare, M.; McLeod, M.C.; Kittayapong, P.; Lavery, J.; Lim, L.H.; Marrelli, M.T.; Nagaraju, J.; Ombongi, K.; Othman, R.Y.; Pillai, V.; Ramsey, J.; Reuben, R.; Rose, R.I.; Tyagi, B.K.; Mumford, J.,  AsPac J. Mol. Biol. Biotechnol.,  17:75-85. 2009.
In the ongoing fight against vectors of human diseases, disease endemic countries (DECs) may soon benefit from innovative control strategies involving modified insect vectors. For instance, three promising methods (viz. RIDL® [Release of Insects with a Dominant Lethal], ...
Keywords: , , ,

Researchers eliminate mosquito population through CRISPR gene editing technology

Goss, L,  Pharmafile,  2008.
Researchers at Imperial College London have been able to wipe out a population of mosquitoes through ‘gene drive’ technology. The study, published in the journal Nature Biotechnology saw a CRISPR edited gene being used to prevent female mosquitos from being born.; ; As such ...
Keywords: , , ,

Targeting the X chromosome during spermatogenesis induces Y chromosome transmission ratio distortion and early dominant embryo lethality in Anopheles gambiae

Windbichler, NP, P. A.; Crisanti, A.,  PLOS Genetics,  4:1-9. 2008.
We have exploited the high selectivity of the homing endonuclease I-PpoI for the X-linked Anopheles gambiae 28S ribosomal genes to selectively target X chromosome carrying spermatozoa. Our data demonstrated that in heterozygous males, the expression of I-PpoI in the testes ...
Keywords: , , ,

Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryos

Windbichler, NP, P. A.; Catteruccia, F.; Ranson, H.; Burt, A.; Crisanti, A.,  Nucleic Acids Research,  35:5922-5933. 2007.
Homing endonuclease genes (HEGs) are selfish genetic elements that combine the capability to selectively disrupt specific gene sequences with the ability to rapidly spread from a few individuals to an entire population through homologous recombination repair events. Because of ...
Keywords: , , ,

Meiotic drive by the Y-linked D gene in Aedes aegypti (L.) (Diptera : Culicidae) is associated with disruption of spermiogenesis, leading to premature senescence of spermatozoa

Owusu-Daaku, KOB, R. D.; Wood, R. J.,  Arthropod Structure & Development,  36:233-243. 2007.
Y chromosome meiotic drive in the mosquito Aedes aegypti, due to the gene D (Distorter) in coupling with M (male determination) [the MD haplotype], is associated with spermiogenic disruption, leading to senescence, at a rate Proportionate to male excess. Spermiogenesis was ...
Keywords: , , ,

Malaria Control with Genetically Manipulated Insect Vectors

L. Alphey, C. B. Beard, P. Billingsley, M. Coetzee, A. Crisanti, C. Curtis, P. Eggleston, C. Godfray, J. Hemingway, M. Jacobs-Lorena, A. A. James, F. C. Kafatos, L. G. Mukwaya, M. Paton, J. R. Powell, W. Schneider, T. W. Scott, B. Sina, R. Sinden, S. Sink,  Science,  298:119. 2002.
At a recent workshop, experts discussed the benefits, risks, and research priorities associated with using genetically manipulated insects in the control of vector-borne diseases.
Keywords: , , ,

Variation in Y chromosome meiotic drive in Aedes aegypti (Diptera: Culicidae): a potential genetic approach to mosquito control

OwusuDaaku, KOW, R. J.; Butler, R. D.,  Bulletin of Entomological Research,  87:617-623. 1997.
Reciprocal crosses between strains of Aedes aegypti (Linnaeus) from different geographical areas have revealed an unexpectedly complex pattern of holandrically inherited male biased sex ratios in F2. The variation has been interpreted in terms of a web of X-Y interactions in F1, ...
Keywords: , , ,

Combining the meiotic drive gene-D and the translocation T-1 in the mosquito, Aedes aegypti(L) .2: Recombination

Pearson, AMW, R. J.,  Genetica,  54:79-85. 1980.
Recombination on the sex-chromosome of Aedes aegypti has been studied in male genotypes incorporating the sex-linked translocation T1 and the meiotic drive gene D from three different strains (Trinidad, Bozo and Caracas).
Keywords: , , ,