Keywords: wolbachia

A single mutation weakens symbiont-induced reproductive manipulation through reductions in deubiquitylation efficiency

J. F. Beckmann, K. Van Vaerenberghe, D. E. Akwa and B. S. Cooper,  Proceedings of the National Academy of Sciences,  118:e2113271118. 2021.
We show that a single naturally observed mutation weakens CI by reducing deubiquitylation. These discoveries help elucidate the molecular basis of symbiont-induced reproductive manipulations.Animals interact with microbes that affect their performance and fitness, including ...
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Discrete dynamical models on Wolbachia infection frequency in mosquito populations with biased release ratios

Y. Shi and B. Zheng,  Journal of Biological Dynamics,  2021.
We develop two discrete models to study how supplemental releases affect the Wolbachia spreading dynamics in cage mosquito populations. The first model focuses on the case when only infected males are released at each generation. This release strategy has been proved to be ...
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Symbiotic Interactions Between Mosquitoes and Mosquito Viruses

M. Altinli, E. Schnettler and M. Sicard,  Front Cell Infect Microbiol,  11:694020. 2021.
Mosquitoes not only transmit human and veterinary pathogens called arboviruses (arthropod-borne viruses) but also harbor mosquito-associated insect-specific viruses (mosquito viruses) that cannot infect vertebrates. In the past, studies investigating mosquito viruses mainly ...
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Two newly introduced Wolbachia endosymbionts induce cell host differences in competitiveness and metabolic responses

T. P. Li, S. S. Zha, C. Y. Zhou, X. Xia, A. A. Hoffmann and X. Y. Hong,  Appl Environ Microbiol,  Aem0147921. 2021.
Wolbachia endosymbionts can induce multiple reproductive manipulations in their hosts, with cytoplasmic incompatibility (CI) being one of the most common manipulations. The important agricultural pests, white-backed planthopper (Sogatella furcifera) and brown planthopper ...
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Persistent Spodoptera frugiperda rhabdovirus infection in Sf9 cells is not restricted by Wolbachia wMelPop-CLA and wAlbB strains and is targeted by the RNAi machinery

R. Parry, H. de Malmanche and S. Asgari,  Virology,  563:82-87. 2021.
The endosymbiotic bacterium Wolbachia pipientis confers RNA virus refractoriness in Drosophila and Aedes mosquitoes. Questions remain about the Wolbachia-virus restriction phenotype and how extensive this phenomenon may be within other arthropods. Here, we generated two ...
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Wolbachia-Conferred Antiviral Protection Is Determined by Developmental Temperature

E. Chrostek, N. Martins, M. S. Marialva and L. Teixeira,  mBio,  e0292320. 2021.
Overall, we show that Wolbachia-conferred antiviral protection is temperature dependent, being present or absent depending on the environmental conditions. This interaction likely impacts Wolbachia-host interactions in nature and, as a result, frequencies of host and symbionts in ...
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Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release

N. Pocquet, O. O’Connor, H. A. Flores, J. Tutagata, M. Pol, D. J. Hooker, C. Inizan, S. Russet, J. M. Duyvestyn, E. C. Pacidônio, D. Girault, D. da Silva Gonçalves, M. Minier, F. Touzain, E. Chalus, K. Lucien, F. Cheilan, T. Derycke, S. Laumond, C. P. Sim,  PLOS Neglected Tropical Diseases,  15:e0009752. 2021.
Dengue represents a risk for almost half of the world’s population, especially throughout the tropics. In New Caledonia, dengue outbreaks have become more frequent in the past decade along with the recent circulation of chikungunya and Zika viruses. The opportunity to use the ...
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wMel Wolbachia genome remains stable after 7 years in Australian Aedes aegypti field populations

K. R. Dainty, J. Hawkey, L. M. Judd, E. C. Pacidônio, J. M. Duyvestyn, D. S. Gonçalves, S. Y. Lin, T. B. O'Donnell, S. L. O'Neill, C. P. Simmons, K. E. Holt and H. A. Flores,  Microbial Genomics,  7. 2021.
Infection of wMel Wolbachia in Aedes aegypti imparts two signature features that enable its application for biocontrol of dengue. First, the susceptibility of mosquitoes to viruses such as dengue and Zika is reduced. Second, a reproductive manipulation is caused that enables wMel ...
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Millions of Lab-Grown Mosquitoes Are Being Released in Guangzhou

F. Yiying,  Sixth Tone,  2021.
Guangzhou is releasing millions of lab-engineered mosquitoes every day to neuter and prevent preexisting mosquitoes in the environment from spreading vector-borne diseases, local television station reported Saturday.The Guangzhou Wolbaki Biotech Co., Ltd., in partnership with the ...
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Sex separation of Aedes spp. mosquitoes for sterile insect technique application: a review

B. M. Moran-Aceves, C. F. Marina, A. Dor, P. Liedo and J. Toledo,  Entomologia Experimentalis Et Applicata,  10. 2021.
Separation of the sexes is necessary for the application of the sterile insect technique (SIT) in mosquitoes due to the hematophagous habits and disease vector activity of the females. In this review we analyze the history, current status, and future perspectives for the ...
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Wolbachia as translational science: controlling mosquito-borne pathogens

E. P. Caragata, H. L. C. Dutra, P. H. F. Sucupira, A. G. A. Ferreira and L. A. Moreira,  Trends in Parasitology,  2021.
In this review we examine how exploiting the Wolbachia?mosquito relationship has become an increasingly popular strategy for controlling arbovirus transmission. Field deployments of Wolbachia-infected mosquitoes have led to significant decreases in dengue virus incidence via high ...
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Combating mosquito-borne diseases using genetic control technologies

G.-H. Wang, S. Gamez, R. R. Raban, J. M. Marshall, L. Alphey, M. Li, J. L. Rasgon and O. S. Akbari,  Nature Communications,  12:4388. 2021.
Mosquito-borne diseases, such as dengue and malaria, pose significant global health burdens. Unfortunately, current control methods based on insecticides and environmental maintenance have fallen short of eliminating the disease burden. Scalable, deployable, genetic-based ...
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A wAlbB Wolbachia&lt transinfection displays stable phenotypic effects across divergent Aedes aegypti mosquito backgrounds

P. A. Ross, X. Gu, K. L. Robinson, Q. Yang, E. Cottingham, Y. Zhang, H. L. Yeap, X. Xu, N. M. Endersby-Harshman and A. A. Hoffmann,  bioRxiv,  2021.06.25.450002. 2021.
Aedes mosquitoes harboring intracellular Wolbachia bacteria are being released in arbovirus and mosquito control programs. With releases taking place around the world, understanding the contribution of host variation to Wolbachia phenotype is crucial. We generated a Wolbachia ...
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Manipulated Mosquitoes Cut Dengue by 77%

T. Hayes,  Healthcare Packaging,  2021.
Dengue, a mosquito-borne viral disease, wasn’t that common 50 years ago. In fact, only nine countries had severe outbreaks. But since then, it’s been on a steady incline to the point that there are now 400 million infections a year that contribute to 22,000 deaths. ...
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Dengue fever: Upstaged but not outmatched by COVID-19

C. E. Baclig,  INQUIRER.NET,  2021.
Science has made gains in the war on dengue and other diseases that mosquitoes carry, like malaria.One of these is the World Mosquito Program (WMP), a non-profit initiative that aims to protect the global community from mosquito-borne viral diseases, by deploying a natural ...
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Dengue Infections Can Be Sharply Reduced With Wolbachia Bacteria

J. Stone,  Medscape,  2021.
A modestly titled new study released in the New England Journal of Medicine belies the extraordinary 77% protective efficacy reported for preventing dengue infections with Wolbachia-infected Aedes aegypti mosquitoes. A cluster-randomized clinical trial, the AWED ("Applying ...
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Making mosquitoes to fight mosquitoes to prevent dengue

A. George,  Times of India,  2021.
In 2017, Delhi Chief Minister Arvind Kejriwal tweeted that the national capitl needed to be made mosquito-free. The same year, his Kerala counterpart, Pinaray Vijayan, called a three-day state-wide cleanliness drive as hospitals filled with genue patients.
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Stable high-density and maternally inherited Wolbachia infections in Anopheles moucheti and Anopheles demeilloni mosquitoes

T. Walker, S. Quek, C. L. Jeffries, J. Bandibabone, V. Dhokiya, R. Bamou, M. Kristan, L. A. Messenger, A. Gidley, E. A. Hornett, E. R. Anderson, C. Cansado-Utrilla, S. Hegde, C. Bantuzeko, J. C. Stevenson, N. F. Lobo, S. C. Wagstaff, C. A. Nkondjio, S. R.,  Current Biology,  31:2310. 2021.
Wolbachia, a widespread bacterium that can reduce pathogen transmission in mosquitoes, has recently been reported to be present in Anopheles (An.) species. In wild populations of the An. gambiae complex, the primary vectors of Plasmodium malaria in Sub-Saharan Africa, Wolbachia ...
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Using Wolbachia to Eliminate Dengue: Will the Virus Fight Back?

M. Edenborough Kathryn, A. Flores Heather, P. Simmons Cameron, E. Fraser Johanna and C. Pierson Ted,  Journal of Virology,  95:e02203-20. 2021.
Recent fieldtrials havedemonstratedthatdengue incidence can besubstantially reduced by introgressing strains of the endosymbiotic bacterium Wolbachia into Aedes aegypti mosquito populations. This strategy relies on Wolbachia reducing the susceptibility of Ae. aegypti to ...
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Dengue Fever Cut Down by 77% With Groundbreaking Bacteria-Armed Mosquitoes

M. Davis,  The Science Times,  2021.
Scientists found that dengue fever cases have decreased by 77% in a groundbreaking trial that took place in Yogyakarta City, Indonesia. They used Wolbachia-infected mosquitoes that reduced their ability to spread the dengue fever. The team at the World Mosquito Program said that ...
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Mosquito ‘bacteria hack’ nearly eliminates dengue fever and could save millions of lives

A. Wilkins,  METRO,  2021.
Mosquitoes infected with a ‘miraculous’ bacteria have been shown to reduce dengue fever cases by 77%, in a groundbreaking new study. Scientists released mosquitoes infected with ‘Wolbachia’ bacteria into the Indonesian city of Yogyakarta – but only in certain zones. In ...
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‘Miraculous’ mosquito hack cuts dengue by 77%

J. Gallagher,  BBC,  2021.
Dengue fever cases have been cut by 77% in a "groundbreaking" trial that manipulates the mosquitoes that spread it, say scientists. They used mosquitoes infected with "miraculous" bacteria that reduce the insect's ability to spread dengue. The trial took place in Yogyakarta city, ...
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Modified mosquitoes reduce dengue cases by 77% in Indonesia experiment

M. Fox,  CNN,  2021.
An experiment to infect mosquitoes with bacteria that stop them from transmitting viruses appears to have helped reduced the spread of deadly dengue virus in Indonesia, researchers reported Wednesday. The modified mosquitoes thrived for three years, and cases of dengue were ...
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Efficacy of Wolbachia-Infected Mosquito Deployments for the Control of Dengue

A. Utarini, C. Indriani, R. A. Ahmad, W. Tantowijoyo, E. Arguni, M. R. Ansari, E. Supriyati, D. S. Wardana, Y. Meitika, I. Ernesia, I. Nurhayati, E. Prabowo, B. Andari, B. R. Green, L. Hodgson, Z. Cutcher, E. Rancès, P. A. Ryan, S. L. O’Neill, S. M. Dufau,  New England Journal of Medicine,  384:2177-2186. 2021.
BACKGROUND Aedes aegypti mosquitoes infected with the wMel strain of Wolbachia pipientis are less susceptible than wild-type A. aegypti to dengue virus infection. METHODS We conducted a cluster-randomized trial involving releases of wMel-infected A. aegypti mosquitoes for the ...
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A Pivotal Mosquito Experiment Could Not Have Gone Better

E. Yong,  The Atlantic,  2021.
The World Mosquito Program (WMP), a nonprofit that pioneered this technique, had run small pilot studies in Australia that suggested it could work. Utarini, who co-leads WMP Yogyakarta, has now shown conclusively that it does. Her team released Wolbachia-carrying mosquitoes in ...
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Mosquitoes armed with virus-fighting bacteria sharply curb dengue infections, hospitalizations

K. Servick,  Science,  2021.
A strategy for fighting dengue fever with bacteria-armed mosquitoes has passed its most rigorous test yet: a large, randomized, controlled trial. Researchers reported today dramatic reductions in rates of dengue infection and hospitalization in areas of an Indonesian city where ...
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Study demonstrates ‘exciting potential’ of Wolbachia-infected mosquitoes to control dengue

G. Gallagher,  Healio,  2021.
The release of Wolbachia-infected mosquitoes led to a 77% reduction in the incidence of symptomatic dengue in an Indonesian city, according to researchers, who said the same approach could be used to fight other mosquito-borne diseases. The study tested a strain of Wolbachia ...
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Vector control: Discovery of Wolbachia in malaria vectors

P. A. Ross and A. A. Hoffmann,  Current Biology,  31:R738-R740. 2021.
Wolbachia bacteria are being widely released for suppression of dengue transmitted by Aedes mosquitoes. Walker, Quek, Jeffries and colleagues present robust evidence for natural Wolbachia infections in malaria-vectoring Anopheles mosquitoes, paving the way for new ...
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Living in the endosymbiotic world of Wolbachia: A centennial review

R. Kaur, J. D. Shropshire, K. L. Cross, B. Leigh, A. J. Mansueto, V. Stewart, S. R. Bordenstein and S. R. Bordenstein,  Cell Host and Microbe,  29:879-893. 2021.
The most widespread intracellular bacteria in the animal kingdom are maternally inherited endosymbionts of the genus Wolbachia. Their prevalence in arthropods and nematodes worldwide and stunning arsenal of parasitic and mutualistic adaptations make these bacteria a biological ...
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Mechanistically comparing reproductive manipulations caused by selfish chromosomes and bacterial symbionts

E. Dalla Benetta, O. S. Akbari and P. M. Ferree,  Heredity,  126:707-716. 2021.
Insects naturally harbor a broad range of selfish agents that can manipulate their reproduction and development, often leading to host sex ratio distortion. Such effects directly benefit the spread of the selfish agents. These agents include two broad groups: bacterial symbionts ...
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Modeling and analysis of the implementation of the Wolbachia incompatible and sterile insect technique for mosquito population suppression.

B. Zheng, J. S. Yu and J. Li,  Siam Journal on Applied Mathematics,  81:718-740. 2021.
Mathematical analysis may offer guidance in designing effective mass release strategies for the area-wide application of this Wolbachia incompatible and sterile insect technique in the future. The two most crucial concerns in designing release strategies are how often and in what ...
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Evidence for natural hybridization and novel Wolbachia strain superinfections in the Anopheles gambiae complex from Guinea

C. L. Jeffries, C. Cansado-Utrilla, A. H. Beavogui, C. Stica, E. K. Lama, M. Kristan, S. R. Irish and T. Walker,  Royal Society Open Science,  8:18. 2021.
Wolbachia, a widespread bacterium which can influence mosquito-borne pathogen transmission, has recently been detected within Anopheles (An.) species that are malaria vectors in Sub-Saharan Africa. Although studies have reported Wolbachia strains in the An. gambiae complex, ...
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When More is Less: Mosquito Population Suppression Using Sterile, Incompatible and Genetically Modified Male Mosquitoes

S. L. Dobson,  Journal of Medical Entomology,  58:1980-1986. 2021.
The current review of the Sterile Insect Technique (SIT) is motivated by new technologies and the recent renaissance of male release field trials, which is driving an evolution in mosquito control and regulation. Practitioners that are releasing male mosquitoes would do well to ...
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Number of Project Wolbachia mosquitoes released is constantly reviewed to maintain suppression of dengue: NEA

N. L. Ching,  today,  2021.
Project Wolbachia – Singapore has yielded promising results so far.Releases of non-biting male Wolbachia-Aedes mosquitoes have suppressed the urban Aedes aegypti mosquito populations in study sites at Tampines and Yishun by up to 90 per cent, and we have observed 58 to 74 per ...
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Genetic Biocontrol Webinars

David O'Brochta and Hector Quemada,  GeneConvene Global Collaborative,  2021.
In the mid 20th century various ideas emerged concerning how genetics and genetic principles could be directly applied to age-old problems of managing insects that threaten food security and public health.  This series of webinars will explore the current state-of-the-art of ...
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How to engage communities on a large scale? Lessons from World Mosquito Program in Rio de Janeiro, Brazil [version 2; peer review: 1 approved, 2 approved with reservations]

G. B. Costa, R. Smithyman, S. L. O'Neill and L. A. Moreira,  Gates Open Research,  2021.
Here we discuss and analyse the framework for community engagement implemented by the WMP in Brazil, during the large-scale deployment of the method in the municipalities of Niterói and Rio de Janeiro, Brazil. Our experience indicates that the community engagement work for ...
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Reply to: Issues with combining incompatible and sterile insect techniques

Y. Li, L. A. Baton, D. Zhang, J. Bouyer, A. G. Parker, A. A. Hoffmann, L. C. Ng, C. H. Tan and Z. Xi,  Nature,  590:E3-E5. 2021.
When the aim is elimination of the target population, Uni-CI and Bi-CI do not have appreciably different risks of population replacement. The small-scale field trial8 cited by Moretti and Calvitti in their Comment1 does not provide evidence that Bi-CI protects against population ...
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Issues with combining incompatible and sterile insect techniques

R. Moretti and M. Calvitti,  Nature,  590:E1-E2. 2021.
In a recent paper, Zheng at al.1 performed field experiments that tested a strategy of mosquito suppression based on the release of functionally sterile males produced by the combination of reproductive unidirectional cytoplasmic incompatibility (which is induced by the bacterium ...
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Project Wolbachia: Residents are killing the ‘helpful’ mosquitoes, which can be a nuisance

T. J. Cheng,  today,  2021.
In 2019, Dr Amy Khor, then Senior Minister of State for the Environment and Water Resources, said that there was a 90 per cent suppression rate at study sites in Tampines and Yishun from February to November that year. However, certain public housing estates under the project ...
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GeneConvene Webinar Series on: Genetic Biocontrol

David O'Brochta and Hector Quemada,  ,  2021.
In the mid 20th century various ideas emerged concerning how genetics and genetic principles could be directly applied to age-old problems of managing insects that threaten food security and public health. This series of webinars will explore the current state-of-the-art of what ...
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Next-generation tools to control biting midge populations and reduce pathogen transmission

P. Shults, L. W. Cohnstaedt, Z. N. Adelman and C. Brelsfoard,  Parasites and Vectors,  14:31. 2021.
Biting midges of the genus Culicoides transmit disease-causing agents resulting in a significant economic impact on livestock industries in many parts of the world. Localized control efforts, such as removal of larval habitat or pesticide application, can be logistically ...
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Genetic pest management and the background genetics of release strains

P. T. Leftwich, L. G. Spurgin, T. Harvey-Samuel, C. J. E. Thomas, L. C. Paladino, M. P. Edgington and L. Alphey,  Philosophical Transactions of the Royal Society B: Biological Sciences,  376:20190805. 2020.
We discuss issues around strain selection and the potential consequences of such introgression. We conclude that such introgression is probably harmless in almost all circumstances, and could, in theory, provide specific additional benefits to the release programme. We outline ...
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Wolbachia strain wAlbB maintains high density and dengue inhibition following introduction into a field population of Aedes aegypti

N. A. Ahmad, M.-V. Mancini, T. H. Ant, J. Martinez, G. M. R. Kamarul, W. A. Nazni, A. A. Hoffmann and S. P. Sinkins,  Philosophical Transactions of the Royal Society B: Biological Sciences,  376:20190809. 2020.
Here, wAlbB-carrying Ae. aegypti collected from the field 20 months after the cessation of releases showed no reduction in Wolbachia density or tissue distribution changes compared to a wAlbB laboratory colony. The wAlbB strain continued to induce complete unidirectional ...
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Modelling the Wolbachia incompatible insect technique: strategies for effective mosquito population elimination

D. E. Pagendam, B. J. Trewin, N. Snoad, S. A. Ritchie, A. A. Hoffmann, K. M. Staunton, C. Paton and N. Beebe,  BMC Biology,  18:13. 2020.
We introduce a simple Markov population process model for studying mosquito populations subjected to a Wolbachia-IIT programme which exhibit an unstable equilibrium threshold. The model is used to study, in silico, scenarios that are likely to yield a successful elimination ...
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Reply to: “Enhancement of Aedes aegypti susceptibility to dengue by Wolbachia is not supported”

C. Souto-Maior, J. G. King, L. M. Sartori, R. Maciel-de-Freitas and M. G. M. Gomes,  Nature Communications,  11:6113. 2020.
Ant et al.4 claim that concerns with the data and broader analysis make our conclusions misleading. We herein respond to their comments by demonstrating the robustness of our results to different treatments of the data, and expand our arguments for replacing currently adopted ...
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Enhancement of Aedes aegypti susceptibility to dengue by Wolbachia is not supported

T. H. Ant, M.-V. Mancini, J. Martinez and S. P. Sinkins,  Nature Communications,  11:6111. 2020.
King et al.3 used DENV infection and transmission modelling to reinterpret experimental data from two previous studies4,5. The authors claimed that wMel Wolbachia increase the mean susceptibility of Ae. aegypti to DENV, contradicting various other studies6,7,8,9,10,11,12. Here, ...
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Vector-Focused Approaches to Curb Malaria Transmission in the Brazilian Amazon: An Overview of Current and Future Challenges and Strategies

E. M. Rocha, R. D. Katak, J. C. de Oliveira, M. D. Araujo, B. C. Carlos, R. Galizi, F. Tripet, O. Marinotti and J. A. Souza,  Tropical Medicine and Infectious Disease,  5. 2020.
Here we present an overview on both conventional and novel promising vector-focused tools to curb malaria transmission in the Brazilian Amazon. If well designed and employed, vector-based approaches may improve the implementation of malaria-control programs, particularly in ...
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Selfish genetic elements and male fertility

R. L. Verspoor, T. A. R. Price and N. Wedell,  Philosophical Transactions of the Royal Society B-Biological Sciences,  375:7. 2020.
Selfish genetic elements (SGEs) are diverse and near ubiquitous in Eukaryotes and can be potent drivers of evolution. Here, we discuss SGEs that specifically act on sperm to gain a transmission advantage to the next generation. The diverse SGEs that affect sperm often impose ...
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Resistance to natural and synthetic gene drive systems

T. A. R. Price, N. Windbichler, R. L. Unckless, A. Sutter, J.-N. Runge, P. A. Ross, A. Pomiankowski, N. L. Nuckolls, C. Montchamp-Moreau, N. Mideo, O. Y. Martin, A. Manser, M. Legros, A. M. Larracuente, L. Holman, J. Godwin, N. Gemmell, C. Courret, A. Buc,  Journal of Evolutionary Biology,  2020.
This review summarizes our current knowledge of drive resistance in both natural and synthetic gene drives. We explore how insights from naturally occurring and synthetic drive systems can be integrated to improve the design of gene drives, better predict the outcome of releases ...
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The Evolving Arsenal Against Mosquito-Born Diseases

J. Smith,  Labiotech.eu,  2020.
As the global climate continues to warm, disease-spreading mosquitoes such as Aedes aegypti are expected to establish themselves in the US and Europe.
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Prospects and Pitfalls: Next-Generation Tools to Control Mosquito-Transmitted Disease

E. P. Caragata, S. Dong, Y. Dong, M. L. Simões, C. V. Tikhe and G. Dimopoulos,  Annual Review of Microbiology,  74:455-475. 2020.
A diverse array of next-generation tools has been designed to eliminate mosquito populations or to replace them with mosquitoes that are less capable of transmitting key pathogens.
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How to fight the deadly dengue virus? Make your own mosquitoes

J. Emont,  Wall Street Journal,  2020.
When the bacteria-laden male mosquitoes are released into the open and mate with naturally-born females, the resultant eggs won’t hatch.
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Non-GMO approach reduces cases of mosquito-borne dengue by 77%

GM Watch,  GM Watch,  2020.
A randomized field trial found that mosquitoes infected with a natural bacterium called Wolbachia reduced cases of dengue by an "extraordinary" 77%.
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Bacteria-Laced Mosquitoes Limit Spread of Dengue

A. Heidt,  The Scientist,  2020.
Researchers have infected Aedes aegypti mosquitoes—the species responsible for passing on many diseases—with bacteria called Wolbachia with the intent of reducing the insects’ ability to pass on dengue to people.
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Scientists infect mosquitoes with bacteria to stop the transmission of dengue fever in Indonesia, dropping infection rates by 77 percent

D. Avery,  Daily Mail,  2020.
The team found that dengue infections were 77 percent lower in treated neighborhoods, compared to areas not exposed to the infected insects.
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The mosquito strategy that could eliminate dengue

E. Callaway,  Nature,  2020.
The study, conducted in an Indonesia city, showed that releasing mosquitoes modified to carry a bacterium called Wolbachia, which stops the insects from transmitting some viruses, led to a steep drop in cases of dengue fever.
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Researchers Find New Approach To Control Dengue, Zika By Genetically Modifying Mosquitoes

N. Sharma,  R. Republicworld.com,  2020.
A new study carried out in Indonesia has shown that dengue infection rates decreased in regions where the genetically modified mosquitoes were introduced.
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Australian research takes aim at dengue, another killer virus

E. Connors,  Finanacial Review,  2020.
Australian researchers have teamed up with Indonesian philanthropists to strike a blow against dengue fever, the deadly disease that was a growing scourge in south-east Asia and South America long before COVID-19.
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Australian scientists slash dengue fever in Indonesia by infecting mosquitoes with bacteria

A. Barker,  ABC News,  2020.
Australian scientists may have found the secret to eradicating dengue fever, with a lengthy trial in Indonesia drastically reducing the incidence of the mosquito-borne virus.
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Fighting mosquito-borne diseases… with mosquitoes

N. Gubert and A. Baubeau,  Phys Org,  2020.
For decades, researchers have scratched their heads over how to combat deadly mosquito-borne diseases such as dengue fever.
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Cytoplasmic incompatibility: an autocidal mechanism for mosquito population control

V. Dev,  BugBitten BMC,  2020.
Cytoplasmic incompatibility resulting in non-reciprocal fertility is a naturally occurring phenomenon, but remains unexplored to greater extent for the control of insect vector populations. This mechanism deserves priority for mosquito control and reducing disease transmission, ...
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Modeling the suppression dynamics of Aedes mosquitoes with mating inhomogeneity

M. Huang and L. Hu,  Journal of Biological Dynamics,  14:656-678. 2020.
In this work, we introduce a delay differential equation model with mating inhomogeneity to discuss mosquito population suppression based on Wolbachia. Our analyses show that the wild mosquitoes could be eliminated if either the adult mortality rate exceeds the threshold δ∗A ...
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The Biochemistry of Cytoplasmic Incompatibility Caused by Endosymbiotic Bacteria

H. Chen, M. Zhang and M. Hochstrasser,  Genes,  11. 2020.
Many species of arthropods carry maternally inherited bacterial endosymbionts that can influence host sexual reproduction to benefit the bacterium. The most well-known of such reproductive parasites is Wolbachia pipientis. Wolbachia are obligate intracellular ...
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Artificial Selection Finds New Hypotheses for the Mechanism of Wolbachia-Mediated Dengue Blocking in Mosquitoes

S. A. Ford, I. Albert, S. L. Allen, S. F. Chenoweth, M. Jones, C. Koh, A. Sebastian, L. T. Sigle and E. A. McGraw,  Frontiers in Microbiology,  11:1456. 2020.
We recently used experimental evolution to reveal that Wolbachia-mediated dengue blocking could be selected upon in the A. aegypti host and showed evidence that strong levels of blocking could be maintained by natural selection. In this study, we investigate the genetic variation ...
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Detecting the population dynamics of an autosomal sex ratio distorter transgene in malaria vector mosquitoes

P. Pollegioni, A. R. North, T. Persampieri, A. Bucci, R. L. Minuz, D. A. Groneberg, T. Nolan, P. A. Papathanos, A. Crisanti and R. Muller,  Journal of Applied Ecology,  11. 2020.
A sex-distorting autosomal transgene has been developed recently in G3 mosquitoes, a laboratory strain of the malaria vectorAnopheles gambiaes.l. Following the World Health Organization guidance framework for the testing of GM mosquitoes, we assessed the dynamics of this ...
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Genetic Biocontrol – An Overview

GeneConvene Global Collaborative,,  GeneConvene Global Collaborative,  2020.
This video explains what genetic biocontrol is and surveys various technologies that can be consider genetic biocontrol technologies.  It offers a conceptual organization of the various technologies based on the potential of genetic biocontrol organisms to persist and spread in ...
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Mosquito-Borne Diseases Emergence/Resurgence and How to Effectively Control It Biologically

H. Dahmana and O. Mediannikov,  Pathogens,  9:26. 2020.
Deadly pathogens and parasites are transmitted by vectors and the mosquito is considered the most threatening vector in public health, transmitting these pathogens to humans and animals. We are currently witnessing the emergence/resurgence in new regions/populations of the most ...
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Can we kill the dreaded mosquito? Do we even want to?

Stacey McKenna,  Sierra,  2020.
As a major vector for disease, the mosquito has harmed more human beings than just about any other animal, and a changing climate is only boosting those numbers. As the range of disease-carrying species of mosquitoes expands, so does their ability to transmit the parasites and ...
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Selfish genes and sexual selection: the impact of genomic parasites on host reproduction

N. Wedell,  Journal of Zoology,  311:1-12. 2020.
Selfish genetic elements (SGEs) such as replicating mobile elements, segregation distorters and maternally inherited endosymbionts, bias their transmission success relative to the rest of the genome to increase in representation in subsequent generations. As such, they generate ...
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The potential cost-effectiveness of controlling dengue in Indonesia using wMel Wolbachia released at scale: a modelling study

O. J. Brady, D. D. Kharisma, N. N. Wilastonegoro, K. M. Reilly, E. Hendricx, L. S. Bastos, L. Yakob and D. S. Shepard,  medRxiv,  2020.01.11.20017186. 2020.
Wolbachia releases in high density urban areas is expected to be highly cost-effective and could potentially be the first cost saving intervention for dengue. Sites with strong public health infrastructure, fiscal capacity, and community support should be prioritized.
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Wolbachia transinfections in Culex quinquefasciatus generate cytoplasmic incompatibility

T. H. Ant, C. Herd, F. Louis, A. B. Failloux and S. P. Sinkins,  Insect Molecular Biology,  29:1-8. 2020.
Culex quinquefasciatus is an important mosquito vector of a number of viral and protozoan pathogens of humans and animals, and naturally carries the endosymbiont Wolbachia pipientis, strain wPip. Wolbachia are used in two distinct vector control strategies: firstly, population ...
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The toxin–antidote model of cytoplasmic incompatibility: Genetics and evolutionary implications

Beckmann, J. F., M. Bonneau, H. Chen, M. Hochstrasser, D. Poinsot, H. Merçot, M. Weill, M. Sicard and S. Charlat,  Trends in Genetics,  35:175-185. 2019.
Wolbachia bacteria inhabit the cells of about half of all arthropod species, an unparalleled success stemming in large part from selfish invasive strategies. Cytoplasmic incompatibility (CI), whereby the symbiont makes itself essential to embryo viability, is the most common of ...
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Malaysia Wolbachia trials: Battling dengue and other mosquito-borne viruses

,  2019.

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Establishment of Wolbachia Strain wAlbB in Malaysian Populations of Aedes aegypti for Dengue Control

W. A. Nazni, A. A. Hoffmann, A. NoorAfizah, Y. L. Cheong, M. V. Mancini, N. Golding, G. M. R. Kamarul, M. A. K. Arif, H. Thohir, H. NurSyamimi, M. Z. ZatilAqmar, M. NurRuqqayah, A. NorSyazwani, A. Faiz, F.-R. M. N. Irfan, S. Rubaaini, N. Nuradila, N. M. N,  Current Biology,  29:4241-4248.e5. 2019.
Dengue has enormous health impacts globally. A novel approach to decrease dengue incidence involves the introduction of Wolbachia endosymbionts that block dengue virus transmission into populations of the primary vector mosquito, Aedes aegypti. The wMel Wolbachia strain has ...
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Incompatible and sterile insect techniques combined eliminate mosquitoes

X. Zheng, D. Zhang, Y. Li, C. Yang, Y. Wu, X. Liang, Y. Liang, X. Pan, L. Hu, Q. Sun, X. Wang, Y. Wei, J. Zhu, W. Qian, Z. Yan, A. G. Parker, J. R. L. Gilles, K. Bourtzis, J. Bouyer, M. Tang, B. Zheng, J. Yu, J. Liu, J. Zhuang, Z. Hu, M. Zhang, J.-T. Gon,  572,  56-61. 2019.
Here we show that combining incompatible and sterile insect techniques (IIT–SIT) enables near elimination of field populations of the world’s most invasive mosquito species, Aedes albopictus. Millions of factory-reared adult males with an artificial triple-Wolbachia infection ...
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Engineered resistance to Zika virus in transgenic Aedes aegypti expressing a polycistronic cluster of synthetic small RNAs

Buchman, AG, S.; Li, M.; Antoshechkin, I.; Li, H. H.; Wang, H. W.; Chen, C. H.; Klein, M. J.; Duchemin, J. B.; Paradkar, P. N.; Akbari, O. S.,  Proceedings of the National Academy of Sciences of the United States of America,  116:3656-3661. 2019.
Recent Zika virus (ZIKV) outbreaks have highlighted the necessity for development of novel vector control strategies to combat arboviral transmission, including genetic versions of the sterile insect technique, artificial infection with Wolbachia to reduce population size and/or ...
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The toxin–antidote model of cytoplasmic incompatibility: Genetics and evolutionary implications

Beckmann, JFB, Manon; Chen, Hongli; Hochstrasser, Mark; Poinsot, Denis; Merçot, Hervé; Weill, Mylène; Sicard, Mathieu; Charlat, Sylvain,  Trends in Genetics,  35:175-185. 2019.
Wolbachia bacteria inhabit the cells of about half of all arthropod species, an unparalleled success stemming in large part from selfish invasive strategies. Cytoplasmic incompatibility (CI), whereby the symbiont makes itself essential to embryo viability, is the most common of ...
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Two-By-One model of cytoplasmic incompatibility: Synthetic recapitulation by transgenic expression of cifA and cifB in Drosophila

Shropshire, JDB, S. R.,  PLOS Genetics,  15:e1008221. 2019.
Wolbachia are maternally inherited bacteria that infect arthropod species worldwide and are deployed in vector control to curb arboviral spread using cytoplasmic incompatibility (CI). CI kills embryos when an infected male mates with an uninfected female, but the lethality is ...
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One prophage WO gene rescues cytoplasmic incompatibility in Drosophila melanogaster

Shropshire, J. D., J. On, E. M. Layton, H. Zhou and S. R. Bordenstein,  Proceedings of the National Academy of Sciences,  115:4987. 2018.
The World Health Organization recommended pilot deployment of Wolbachia-infected mosquitoes to curb viral transmission to humans. Releases of mosquitoes are underway worldwide because Wolbachia can block replication of these pathogenic viruses and deterministically spread by a ...
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Evolutionary genetics of cytoplasmic incompatibility genes cifA and cifB in prophage WO of Wolbachia

Lindsey, A. R. I., D. W. Rice, S. R. Bordenstein, A. W. Brooks, S. R. Bordenstein and I. L. G. Newton,  Genome Biology and Evolution,  10:434-451. 2018.
The bacterial endosymbiont Wolbachia manipulates arthropod reproduction to facilitate its maternal spread through host populations. The most common manipulation is cytoplasmic incompatibility (CI): Wolbachia-infected males produce modified sperm that cause embryonic mortality, ...
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Rapid comeback of males: evolution of male-killer suppression in a green lacewing population

Hayashi, MN, M.; Kageyama, D.,  Proceedings of the Royal Society B-Biological Sciences,  285:6. 2018.
Evolutionary theory predicts that the spread of cytoplasmic sex ratio distorters leads to the evolution of host nuclear suppressors, although there are extremely few empirical observations of this phenomenon. Here, we demonstrate that a nuclear suppressor of a cytoplasmic male ...
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Genetics and genomics of an unusual selfish sex ratio distortion in an insect

Hamilton, PTH, C. N.; Curtis, C. I.; Perlman, S. J.,  Current Biology,  28:3864-3870. 2018.
Diverse selfish genetic elements have evolved the ability to manipulate reproduction to increase their transmission, and this can result in highly distorted sex ratios [1]. Indeed, one of the major explanations for why sex determination systems are so dynamic is because they are ...
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SCIENTIFIC OPINION: In response to the referral of 12 October 2015 concerning use of genetically modified mosquitoes for vector control

High Council for Biotechnology,  High Council for Biotechnology (France),  2017.
The Scientific Committee’s opinion describes emerging vector control techniques using GM mosquitoes, the current state of research into and development of these techniques and the outcomes of initial experiments worldwide. To date, only one technique has been developed to an ...
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A Wolbachia deubiquitylating enzyme induces cytoplasmic incompatibility

Beckmann, J. F., J. A. Ronau and M. Hochstrasser,  Nature Microbiology,  2:17007. 2017.
Wolbachia are obligate intracellular bacteria1 that infect arthropods, including approximately two-thirds of insect species2. Wolbachia manipulate insect reproduction by enhancing their inheritance through the female germline. The most common alteration is cytoplasmic ...
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Genetic conflicts: the usual suspects and beyond

McLaughlin, RNM, H. S.,  Journal of Experimental Biology,  220:6-17. 2017.