Keywords: incompatible insect technique

Effect of Wolbachia Infection and Adult Food on the Sexual Signaling of Males of the Mediterranean Fruit Fly Ceratitis capitata

G. A. Kyritsis, P. Koskinioti, K. Bourtzis and N. T. Papadopoulos,  Insects,  13. 2022.
Sexual signaling is a fundamental component of sexual behavior of Ceratitis capitata that highly determines males' mating success. Nutritional status and age are dominant factors known to affect males' signaling performance and define the female decision to accept a male as a ...
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Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines

C. Sadanandane, K. Gunasekaran, D. Panneer, S. K. Subbarao, M. Rahi, B. Vijayakumar, V. Athithan, A. Sakthivel, S. Dinesh and P. Jambulingam,  Frontiers in Microbiology,  13:947857. 2022.
Wolbachia, an intracellular maternally transmitted endosymbiont, has been shown to interfere with the replication of dengue virus in Aedes aegypti mosquitoes. The Wolbachia-transinfected Ae. aegypti has been currently released in many countries to test its effectiveness in ...
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Wolbachia Dynamics in Mosquitoes with Incomplete CI and Imperfect Maternal Transmission by a DDE System

Y. Su, B. Zheng and X. Zou,  Bulletin of Mathematical Biology,  84:95. 2022.
In this paper, we propose a delay differential equation model to describe the Wolbachia infection dynamics in mosquitoes in which the key factor of cytoplasmic incompactibility (CI) is incorporated in a more natural way than those in the literature. By analyzing the dynamics of ...
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Mosquito control to save Hawaiian honeycreepers does not involve GMOs

Department of Land and Natural Resources,  Hawaii Department of Land and Natural Resources,  2022.
Despite misinformation circulating on social media, the importation of “incompatible-male” mosquitoes to control populations of wild mosquitoes and to save four native bird species from extinction, does not involve the use of any genetically modified organisms (GMOs) or ...
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Establishment of Wolbachia infection in Aedes aegypti from Pakistan via embryonic microinjection and semi-field evaluation of general fitness of resultant mosquito population

M. S. Sarwar, N. Jahan, A. Ali, H. K. Yousaf and I. Munzoor,  Parasites and Vectors,  15:191. 2022.
BACKGROUND: Dengue is a mosquito-borne viral disease that is mainly spread by Aedes aegypti. It is prevalent on five continents, predominantly in tropical and sub-tropical zones across the world. Wolbachia bacteria have been extensively used in vector control strategies ...
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Wolbachia interacts with the microbiome to shape fitness-associated traits during seasonal adaptation in Drosophila melanogaster

L. P. Henry, M. Fernandez, S. Wolf and J. Ayroles,  bioRxiv,  2022.05.31.494239. 2022.
The microbiome contributes to many different host traits, but its role in host adaptation remains enigmatic. The fitness benefits of the microbiome often depend on ecological conditions, but fluctuations in both the microbiome and environment modulate these fitness benefits. ...
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Reply to: Assessing the efficiency of Verily’s automated process for production and release of male Wolbachia-infected mosquitoes

J. E. Crawford, K. C. Hopkins, A. Buchman, T. Zha, P. Howell, E. Kakani, J. R. Ohm, N. Snoad, L. Upson, J. Holeman, P. Massaro, S. L. Dobson, F. S. Mulligan and B. J. White,  Nature Biotechnology,  2022.
We appreciate the comments from Bouyer et al. under their mandate as a United Nations agency program (‘to promote the safe and appropriate use of nuclear techniques and related technologies in food and agriculture’) on our paper1 . The centuries-old fight against ...
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Assessing the efficiency of Verily’s automated process for production and release of male Wolbachia-infected mosquitoes

J. Bouyer, H. Maiga and M. J. B. Vreysen,  Nature Biotechnology,  2022.
A paper by Crawford et al.1 titled ‘Efficient production of male Wolbachia-infected Aedes aegypti mosquitoes enables large-scale suppression of wild populations’ reports a mosquito control suppression trial carried out from2017 to 2018 in Fresco Country, California, USA1 . ...
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Strategies to Mitigate Establishment under the Wolbachia Incompatible Insect Technique

S. Soh, S. H. Ho, J. Ong, A. Seah, B. S. Dickens, K. W. Tan, J. R. Koo, A. R. Cook, S. Sim, C. H. Tan, L. C. Ng and J. T. Lim,  Viruses,  14. 2022.
The Incompatible Insect Technique (IIT) strategy involves the release of male mosquitoes infected with the bacterium Wolbachia. Regular releases of male Wolbachia-infected mosquitoes can lead to the suppression of mosquito populations, thereby reducing the risk of transmission of ...
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Pilot trial using mass field-releases of sterile males produced with the incompatible and sterile insect techniques as part of integrated Aedes aegypti control in Mexico

A. Martín-Park, A. Che-Mendoza, Y. Contreras-Perera, S. Pérez-Carrillo, H. Puerta-Guardo, J. Villegas-Chim, G. Guillermo-May, A. Medina-Barreiro, H. Delfín-González, R. Méndez-Vales, S. Vázquez-Narvaez, J. Palacio-Vargas, F. Correa-Morales, G. Ayora-Tal,  PLoS Negl Trop Dis,  16:e0010324. 2022.
We implemented a controlled before-and-after quasi-experimental study in two suburban localities of Yucatan (Mexico): San Pedro Chimay (SPC), which received IIT-SIT, and San Antonio Tahdzibichén used as control. Release of wAlbB Ae. aegypti males at SPC extended for 6 months ...
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Could species-focused suppression of Aedes aegypti, the yellow fever mosquito, and Aedes albopictus, the tiger mosquito, affect interacting predators? An evidence synthesis from the literature

J. A. S. Bonds, C. M. Collins and L.-C. Gouagna,  Pest Management Science,  2022.
The risks of Aedes aegypti and Aedes albopictus nuisance and vector-borne diseases are rising and the adverse effects of broad-spectrum insecticide application has promoted species-specific techniques, such as sterile insect technique (SIT) and other genetic strategies, as ...
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Increased biting rate and decreased Wolbachia density in irradiated Aedes mosquitoes

R. Moretti, E. Lampazzi, C. Damiani, G. Fabbri, G. Lombardi, C. Pioli, A. Desiderio, A. Serrao and M. Calvitti,  Parasites and Vectors,  15:67. 2022.
Releasing considerable numbers of radiation-sterilized males is a promising strategy to suppress mosquito vectors. However, releases may also include small percentages of biting females, which translate to non-negligible numbers when releases are large. Currently, the effects of ...
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Uniqueness and stability of periodic solutions for an interactive wild and Wolbachia-infected male mosquito model

R. Yan and Q. Sun,  Journal of Biological Dynamics,  2022.
We investigate a mosquito population suppression model, which includes the release of Wolbachia-infected males causing incomplete cytoplasmic incompatibility (CI). The model consists of two sub-equations by considering the density-dependent birth rate of wild mosquitoes. By ...
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Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression

A. Upadhyay, N. R. Feltman, A. Sychla, A. Janzen, S. R. Das, M. Maselko and M. Smanski,  eLife,  11. 2022.
Engineered Genetic Incompatibility (EGI) is a method to create species-like barriers to sexual reproduction. It has applications in pest control that mimic Sterile Insect Technique when only EGI males are released. This can be facilitated by introducing conditional ...
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Recently introduced Wolbachia reduces bacterial species richness and reshapes bacterial community structure in Nilaparvata lugens

T.-P. Li, C.-Y. Zhou, J.-T. Gong, Z. Xi and X.-Y. Hong,  Pest Management Science,  2022.
BACKGROUND Wolbachia has been developed as an effective tool to suppress insect pests and arbovirus transmission. Recently, the brown planthopper Nilaparvata lugens, a serious agricultural pest, has been successfully transinfected with Wolbachia strain wStri from Laodelphax ...
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Lab-scale characterization and semi-field trials of Wolbachia Strain wAlbB in a Taiwan Wolbachia introgressed Ae. aegypti strain

W. L. Liu, H. Y. Yu, Y. X. Chen, B. Y. Chen, S. N. Leaw, C. H. Lin, M. P. Su, L. S. Tsai, Y. Chen, S. H. Shiao, Z. Y. Xi, A. C. C. Jang and C. H. Chen,  PLOS Neglected Tropical Diseases,  16:24. 2022.
Author summaryPrior to open field release, new genetic approaches that interfere with mosquito abilities and reduce mosquito population density require progressive evaluation both in the laboratory and contained field trials. Trials in contained outdoor systems are thus an ...
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Determinants of stakeholders’ attitudes and intentions toward supporting the use of Wolbachia-infected Aedes mosquitoes for dengue control

A. F. Arham, L. Amin, M. A. C. Mustapa, Z. Mahadi, M. Yaacob and M. Ibrahim,  BMC Public Health,  21:2314. 2021.
BACKGROUND: A recent approach in controlling dengue is by using the Wolbachia-infected Aedes mosquito (WiAM). The approach has been reported to be more effective than traditional methods, such as fogging. Therefore, it is imperative to assess the factors predicting its acceptance ...
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Perspectives into Genetic Manipulations for Control of Dengue Vector (Aedes aegypti Linnaeus, 1762) with Reference to Progress in Indian Experiments

R. Chatterjee, S. Bhattacharya and B. K. Tyagi,  Genetically Modified and other Innovative Vector Control Technologies,  2021.
Vector-borne diseases like malaria, dengue, chikungunya, Japanese encephalitis, Zika and others claim millions of lives across the globe annually, and as such their control has become an ardent necessity. Past attempts over the decades have introduced vector control through ...
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Wolbachia Endosymbiont and Mosquito Vectors, with Emphasis on Lymphatic Filariasis Elimination

I. P. Sunish,  Genetically Modified and other Innovative Vector Control Technologies,  2021.
Wolbachia are maternally inherited intracellular bacteria, known to alter early development and mitotic processes in their hosts. They are frequently observed as a reproductive parasite, capable of inducing feminization, parthenogenesis, male killing, or cytoplasmic ...
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Field Trials of Gene Drive Mosquitoes: Lessons from Releases of Genetically Sterile Males and Wolbachia-infected Mosquitoes

J. M. Marshall and V. N. Vásquez,  Genetically Modified and other Innovative Vector Control Technologies,  2021.
The discovery of CRISPR-based gene editing and its application to homing-based gene drive has been greeted with excitement, for its potential to control mosquito-borne diseases on a wide scale, and concern, for the invasiveness and potential irreversibility of a release. At the ...
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Arthropods of Medical Importance: Need for Genetic and Other Innovative Vector Control Technologies, with Emphasis on Eco-biosocial and Environmental Considerations.

B. K. Tyagi,  Genetically Modified and other Innovative Vector Control Technologies,  2021.
Among the world’s known vector groups, viz. arthropods, snails and rodents, the most important vectors originate from arthropods, the jointed legs. Arthropods are doubtlessly regarded as the most dominant creatures on the Earth due largely to their remarkable structural and ...
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The Effects of Boric Acid Sugar Bait on Wolbachia Trans-Infected Male Aedes albopictus (ZAP Males®) in Laboratory Conditions

V. S. Aryaprema, W. A. Qualls, K. L. Dobson, S. L. Dobson and R.-D. Xue,  Insects,  13. 2021.
The field release of Wolbachia trans-infected male mosquitoes, as well as the use of toxic sugar baits, is a novel and promising candidate technique for integrated mosquito management programs. However, the methods of action of the two techniques may not be complementary, because ...
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Genetically Modified and other Innovative Vector Control Technologies

B. K. Tyagi,  SpringerLink,  2021.
This book comprehensively covers the latest development in developing and deploying the genetically modified vectors, particularly Anopheles and Aedes mosquitoes responsible for transmitting malaria parasites and dengue viruses, the most deadly and/or debilitating among all the ...
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New molecular genetic techniques: regulatory and societal considerations

Nielsen, K. M.,  AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application,  2021.
A rapidly expanding toolbox of techniques available for genome editing provides the basis for a new continuum in types of modifications that can be introduced into a genome and blur the bimodal GMO vs. non-GMO (genetically modified organism) divide. Site-directed nucleases (SDN) ...
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Combined sterile insect technique and incompatible insect technique: concept, study design, experience and lessons learned from a pilot suppression trial in Thailand

Kittayapong, P.,  AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application,  2021.
Climate change, rapid global transport and land use change leading to urbanization and agricultural intensification have facilitated disease emergence in vulnerable regions like Southeast Asia, and also the global expansion of vectors and vector-borne diseases into other regions ...
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Barriers and facilitators of area-wide management including sterile insect technique application: The example of Queensland fruit fly

Mankad, A., Loechel, B., and Measham, P. F.,  AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application,  2021.
The area-wide management (AWM) of highly mobile insect pests such as tephritid fruit flies requires an integrated understanding of technical, social and institutional processes that drive a coordinated approach within a defined area. Furthermore, the success of an AWM programme ...
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Ecology, behaviour and area-wide control of the floodwater mosquito Aedes sticticus, with potential of future integration of the sterile insect technique

Lundstrom, J. O. Schafer, M. L. Kittayapong, P.,  AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application,  2021.
The strategy of aerial control of the floodwater mosquito Aedes sticticus (Meigen) in the floodplains of River Dalalven, central Sweden, was developed to directly address specific larval breeding areas in temporary flooded wet meadows and swamps. Using the Bti-based larvicide ...
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Trial suppresses mosquitoes using non-GMO approach

GM Watch,  GM Watch,  2021.
In a first for the Southern Hemisphere, researchers have shown that a bacterium can successfully suppress populations of the invasive, disease-carrying Aedes aegypti mosquito that is responsible for spreading dengue, yellow fever and Zika. Published in PNAS (see abstract below), ...
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Invasive, disease-carrying Aedes aegypti mosquito sterilized with bacteria and eradicated in large-scale trial

CSIRO,  Phys Org,  2021.
In a first for the Southern Hemisphere, researchers have shown a bacteria can successfully sterilize and eradicate the invasive, disease carrying Aedes aegypti mosquito which is responsible for spreading dengue, yellow fever and Zika. The breakthrough could support the ...
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Releasing incompatible males drives strong suppression across populations of wild and Wolbachiat-carrying Aedes aegypti in Australia

N. W. Beebe, D. Pagendam, B. J. Trewin, A. Boomer, M. Bradford, A. Ford, C. Liddington, A. Bondarenco, P. J. De Barro, J. Gilchrist, C. Paton, K. M. Staunton, B. Johnson, A. J. Maynard, G. J. Devine, L. E. Hugo, G. Rasic, H. Cook, P. Massaro, N. Snoad, J.,  Proceedings of the National Academy of Sciences,  118:e2106828118. 2021.
Through replicated treatment and control experiments in northern Australia, regular releases of Aedes aegypti males infected with a Wolbachia from Aedes albopictus was shown to drive strong population suppression in mosaic populations of wild-type (no Wolbachia) and ...
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Wolbachia-mediated sterility suppresses Aedes aegypti populations in the urban tropics

Project Wolbachia-Singapore Consortium,  medRxiv,  2021.
Incompatible insect technique (IIT) via releases of male Wolbachiainfected mosquitoes is a promising tool for dengue control. In a three-year trial in Singaporean high-rise housing estates, we demonstrated that Wolbachia-based IIT dramatically reduces both wildtype Aedes aegypti ...
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Engineered reproductively isolated species drive reversible population replacement

A. Buchman, I. Shriner, T. Yang, J. Liu, I. Antoshechkin, J. M. Marshall, M. W. Perry and O. S. Akbari,  Nature Communications,  12:3281. 2021.
Engineered reproductive species barriers are useful for impeding gene flow and driving desirable genes into wild populations in a reversible threshold-dependent manner. However, methods to generate synthetic barriers are lacking in advanced eukaryotes. Here, to overcome this ...
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Modeling and analysis of the implementation of the Wolbachia incompatible and sterile insect technique for mosquito population suppression.

B. Zheng, J. S. Yu and J. Li,  Siam Journal on Applied Mathematics,  81:718-740. 2021.
Mathematical analysis may offer guidance in designing effective mass release strategies for the area-wide application of this Wolbachia incompatible and sterile insect technique in the future. The two most crucial concerns in designing release strategies are how often and in what ...
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The Insect Pest Control Laboratory of the Joint FAO/IAEA Programme: Ten Years (2010–2020) of Research and Development, Achievements and Challenges in Support of the Sterile Insect Technique

M. J. B. Vreysen, A. M. M. Abd-Alla, K. Bourtzis, J. Bouyer, C. Caceres, C. de Beer, D. Oliveira Carvalho, H. Maiga, W. Mamai, K. Nikolouli, H. Yamada and R. Pereira,  Insects,  12. 2021.
The Joint FAO/IAEA Centre (formerly called Division) of Nuclear Techniques in Food and Agriculture was established in 1964 and its accompanying laboratories in 1961. One of its subprograms deals with insect pest control, and has the mandate to develop and implement the sterile ...
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When More is Less: Mosquito Population Suppression Using Sterile, Incompatible and Genetically Modified Male Mosquitoes

S. L. Dobson,  Journal of Medical Entomology,  58:1980-1986. 2021.
The current review of the Sterile Insect Technique (SIT) is motivated by new technologies and the recent renaissance of male release field trials, which is driving an evolution in mosquito control and regulation. Practitioners that are releasing male mosquitoes would do well to ...
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Number of Project Wolbachia mosquitoes released is constantly reviewed to maintain suppression of dengue: NEA

N. L. Ching,  today,  2021.
Project Wolbachia – Singapore has yielded promising results so far.Releases of non-biting male Wolbachia-Aedes mosquitoes have suppressed the urban Aedes aegypti mosquito populations in study sites at Tampines and Yishun by up to 90 per cent, and we have observed 58 to 74 per ...
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Reply to: Issues with combining incompatible and sterile insect techniques

Y. Li, L. A. Baton, D. Zhang, J. Bouyer, A. G. Parker, A. A. Hoffmann, L. C. Ng, C. H. Tan and Z. Xi,  Nature,  590:E3-E5. 2021.
When the aim is elimination of the target population, Uni-CI and Bi-CI do not have appreciably different risks of population replacement. The small-scale field trial8 cited by Moretti and Calvitti in their Comment1 does not provide evidence that Bi-CI protects against population ...
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Issues with combining incompatible and sterile insect techniques

R. Moretti and M. Calvitti,  Nature,  590:E1-E2. 2021.
In a recent paper, Zheng at al.1 performed field experiments that tested a strategy of mosquito suppression based on the release of functionally sterile males produced by the combination of reproductive unidirectional cytoplasmic incompatibility (which is induced by the bacterium ...
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Project Wolbachia: Residents are killing the ‘helpful’ mosquitoes, which can be a nuisance

T. J. Cheng,  today,  2021.
In 2019, Dr Amy Khor, then Senior Minister of State for the Environment and Water Resources, said that there was a 90 per cent suppression rate at study sites in Tampines and Yishun from February to November that year. However, certain public housing estates under the project ...
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GeneConvene Webinar Series on: Genetic Biocontrol

David O'Brochta and Hector Quemada,  ,  2021.
In the mid 20th century various ideas emerged concerning how genetics and genetic principles could be directly applied to age-old problems of managing insects that threaten food security and public health. This series of webinars will explore the current state-of-the-art of what ...
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A patent review on strategies for biological control of mosquito vector

K. Parihar, M. Telang and A. Ovhal,  World Journal of Microbiology and Biotechnology,  36:23. 2020.
This paper presents a comprehensive technology overview of patent documents disclosing biological agents for mosquito control. The patent analysis revealed that comparable number of patent documents were filed in two technology categories: non-recombinant agents and genetically ...
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Modelling the Wolbachia incompatible insect technique: strategies for effective mosquito population elimination

D. E. Pagendam, B. J. Trewin, N. Snoad, S. A. Ritchie, A. A. Hoffmann, K. M. Staunton, C. Paton and N. Beebe,  BMC Biology,  18:13. 2020.
We introduce a simple Markov population process model for studying mosquito populations subjected to a Wolbachia-IIT programme which exhibit an unstable equilibrium threshold. The model is used to study, in silico, scenarios that are likely to yield a successful elimination ...
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Microbiome Innovation in Agriculture: Development of Microbial Based Tools for Insect Pest Management

M. Qadri, S. Short, K. Gast, J. Hernandez and A. C.-N. Wong,  Frontiers in Sustainable Food Systems,  4. 2020.
This review emphasizes the potential and use of microbes in sustainable insect pest management. We first review the diverse insect traits shaped by insect-microbe associations that span nutrition, immunity, ecological interactions with natural enemy, insecticide resistance, and ...
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How to fight the deadly dengue virus? Make your own mosquitoes

J. Emont,  Wall Street Journal,  2020.
When the bacteria-laden male mosquitoes are released into the open and mate with naturally-born females, the resultant eggs won’t hatch.
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Efficient production of male Wolbachia-infected Aedes aegypti mosquitoes enables large-scale suppression of wild populations

J. E. Crawford, D. W. Clarke, V. Criswell, M. Desnoyer, D. Cornel, B. Deegan, K. Gong, K. C. Hopkins, P. Howell, et al.,  Nature Biotechnology,  38:482-492. 2020.
The range of the mosquito Aedes aegypti continues to expand, putting more than two billion people at risk of arboviral infection. The sterile insect technique (SIT) has been used to successfully combat agricultural pests at large scale, but not mosquitoes, mainly because of ...
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Wolbachia transinfections in Culex quinquefasciatus generate cytoplasmic incompatibility

T. H. Ant, C. Herd, F. Louis, A. B. Failloux and S. P. Sinkins,  Insect Molecular Biology,  29:1-8. 2020.
Culex quinquefasciatus is an important mosquito vector of a number of viral and protozoan pathogens of humans and animals, and naturally carries the endosymbiont Wolbachia pipientis, strain wPip. Wolbachia are used in two distinct vector control strategies: firstly, population ...
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Incompatible and sterile insect techniques combined eliminate mosquitoes

X. Zheng, D. Zhang, Y. Li, C. Yang, Y. Wu, X. Liang, Y. Liang, X. Pan, L. Hu, Q. Sun, X. Wang, Y. Wei, J. Zhu, W. Qian, Z. Yan, A. G. Parker, J. R. L. Gilles, K. Bourtzis, J. Bouyer, M. Tang, B. Zheng, J. Yu, J. Liu, J. Zhuang, Z. Hu, M. Zhang, J.-T. Gon,  572,  56-61. 2019.
Here we show that combining incompatible and sterile insect techniques (IIT–SIT) enables near elimination of field populations of the world’s most invasive mosquito species, Aedes albopictus. Millions of factory-reared adult males with an artificial triple-Wolbachia infection ...
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