Keywords: Incompatible insect technique
Effectiveness of Wolbachia-mediated sterility coupled with sterile insect technique to suppress adult Aedes aegypti populations in Singapore: a synthetic control study
Tags: Aedes, Incompatible insect technique, Sterile insect technique (SIT), WolbachiaBansal, Somya, et al., The Lancet Planetary Health, 8:617-628. 2024.
In this synthetic control study, we conducted a large-scale, field trial of IIT-SIT targeting Aedes aegypti among high-rise public housing estates in Singapore, an equatorial city state. Routinely collected data from a large, nationwide surveillance system of 57 990 unique ...
Incompatible insect technique: insights on potential outcomes of releasing contaminant females: a proof of concept under semi-field conditions
Tags: Incompatible insect technique, WolbachiaGiulia Lombardi, Elena Lampazzi, Maurizio Calvitti, Pest Management Science, 2024.
Releasing large numbers of Aedes albopictus males, carrying the artificially introduced Wolbachia ‘wPip’ strain, results in a decrease in the reproductive capacity of wild females due to a phenomenon known as cytoplasmic incompatibility (CI). This vector control strategy ...
Taking the fight against disease to mosquitoes
Tags: Gene drive, Incompatible insect technique, Mosquitoes, Oxitec, Sterile insect technique (SIT), WolbachiaGregory Devine, Setopati, 2024.
In the medium term it's likely that suppression strategies involving self-limiting genetic modifications, Wolbachia infection and irradiation will be extended to a small number of our most important mosquito vectors of disease.
Efficacy of Wolbachia-mediated sterility to reduce the incidence of dengue: a synthetic control study in Singapore
Tags: Field trials, Incompatible insect technique, WolbachiaJue Tao Lim, Somya Bansal*, Chee Seng Chong*, Borame Dickens, Youming Ng, Lu Deng, Caleb Lee, Li Yun Tan, Grace Chain, Pei Ma, Shuzhen Sim, Cheong Huat Tan, Alex R Cook, Lee Ching Ng, The Lancet Microbe, 2024.
Our study comprised an at-risk population of 607 872 individuals living in intervention sites and 3 894 544 individuals living in control sites. Interventions demonstrated up to 77·28% (121/156, 95% CI 75·81–78·58) intervention efficacy despite incomplete coverage ...
Maui ‘ground zero’ for release of billions of biopesticide lab-altered mosquitoes
Tags: Incompatible insect technique, Malaria, North America, Sterile insect technique (SIT)Michael Nevradakis, LifeSite, 2024.
Up to 775,992,000 bacteria-infected mosquitoes could be released in Maui every week for the next 20 years, according to Hawaii Unites, an environmental advocacy group that last month lost its bid to require the state to conduct an environmental impact statement before allowing ...
Judge sides with Hawaii to block challenge to mosquito release plan
Tags: Birds, Incompatible insect technique, North America, Sterile insect technique (SIT), WolbachiaCandice Cheung, Courthouse News Service, 2024.
A planned program to save endemic Hawaiian birds from avian malaria by releasing thousands of biopesticide mosquitoes into Maui’s rainforest will go on, despite an attempt by an environmental nonprofit to block the project.First Circuit Court Judge John Tonaki ruled Tuesday ...
Different mechanisms of X-ray irradiation-induced male and female sterility in Aedes aegypti
Tags: Aedes, Incompatible insect technique, Mosquitoes, Sterile insect technique (SIT), WolbachiaZhang, H., Trueman, E., Hou, X. et al., BMC Biology, 21. 2023.
Aedes aegypti (Ae. aegypti) is the major vector that transmits many diseases including dengue, Zika, and filariasis in tropical and subtropical regions. Due to the growing resistance to chemical-based insecticides, biological control methods have become an emerging direction to ...
Millions of Mosquitoes Will Rain Down on Hawaii to Save an Iconic Bird
Tags: Biodiversity/Conservation, Birds, Incompatible insect technique, WolbachiaS. Wild, Scientific American, 2023.
Millions of mosquitoes dropped from helicopters could be the greatest hope for Hawaii’s iconic honeycreepers. At least four species of the brightly colored birds could go extinct within the year if no action is taken to save them. “We’re seriously in a race against time at ...
Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast India
Tags: Aedes, Incompatible insect technique, Population modification/replacement, WolbachiaVinayagam, S. Nirmolia, T. Chetry, S. Kumar, N. P. Saini, P. Bhattacharyya, D. R. Bhowmick, I. P. Sattu, K. Patgiri, S. J., Journal of Tropical Medicine, 2023.
Wolbachia, a Gram-negative intracellular bacterium, naturally infects many arthropods, including mosquito vectors responsible for the spread of arboviral diseases such as Zika, chikungunya, and dengue fever. Certain Wolbachia strains are involved in inhibiting arbovirus ...
First Detection and Genetic Identification of Wolbachia Endosymbiont in Field-Caught Aedes aegypti (Diptera: Culicidae) Mosquitoes Collected from Southern Taiwan
Tags: Cytoplasmic incompatibility, Incompatible insect techniqueL.-L. Chao and C.-M. Shih, Microorganisms, 11. 2023.
The prevalence and genetic character of Wolbachia endosymbionts in field-collected Aedes aegypti mosquitoes were examined for the first time in Taiwan. A total of 665 Ae. aegypti were screened for Wolbachia infection using a PCR assay targeting the Wolbachia surface protein (wsp) ...
Modeling the Impact of Migration on Mosquito Population Suppression
Tags: Incompatible insect technique, Modeling, Population suppression, WolbachiaM. Huang and J. Yu, Qualitative Theory of Dynamical Systems, 22:134. 2023.
The Wolbachia-induced incompatible insect technique is a promising strategy for controlling wild mosquito populations. However, recent experimental studies have shown that mosquito migration into target areas dilutes the strategy’s effectiveness. In this work, we formulate a ...
Estimating mosquito abundance and population suppression in an incompatible insect technique study
Tags: Aedes, Incompatible insect technique, ModelingL. Griffin, D. Pagendam, C. Drovandi, B. Trewin and N. W. Beebe, Journal of Applied Ecology, 2023.
Our model can provide valuable insights that can shape decision support systems in sterile insect technique and incompatible insect technique programmes operating over large geographical scales. The model helps determine how many sterile/incompatible insects should be released ...
Draft environmental assessment released for using modified mosquitoes to save native birds on Kauaʻi
Tags: Biodiversity/Conservation, Incompatible insect technique, Malaria, Mosquitoes, North America, WolbachiaAnonymous, Big Island NOW, 2023.
Today, the draft environmental assessment was made public for the use of Wolbachia-based incompatible male mosquitoes on Kauaʻi to stop the spread of avian malaria that is decimating native forest bird populations. The public has 31 days — from June 23 until July 24 — to ...
Draft environmental assessment for use of Wolbachia-based incompatible insect technique for the suppression of nonnative southern house mosquito populations on Kauaʻi
Tags: Biodiversity/Conservation, Culex, Incompatible insect technique, North America, WolbachiaHawai'i Department of Land and Natural Resources, Hawai'i Department of Land and Natural Resources, 2023.
The State of Hawai'i Department of Land and Natural Resources hereby transmits the Draft Environmental Assessment and Anticipated Finding of No Significant Impact (DEAEnvironmental Assessment for use of Wolbachia-based Incompatible Insect Technique for the suppression of ...
Molecular detection of reproductive symbionts and parthenogenesis experiments in Tuta absoluta from Argentina: facing potential for sustainable and specific pest control strategies
Tags: Genetic biocontrol, Incompatible insect technique, Other Symbionts, Sterile insect technique (SIT), WolbachiaC. Cagnotti, C. Conte, J. Kramar, S. Lanzavecchia and S. López, Entomologia Experimentalis et Applicata, 2023.
Tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a key pest of tomato crops. Specific and environmentally friendly control strategies against this pest, such as the sterile insect technique (SIT), are under development and sexual reproduction has emerged ...
The optimal strategy of incompatible insect technique (IIT) using Wolbachia and the application to malaria control
Tags: Incompatible insect technique, Malaria, Modeling, Population suppression, WolbachiaT. Matsufuji and S. Seirin-Lee, Journal of Theoretical Biology, 569:111519. 2023.
For decades, techniques to control vector population with low environmental impact have been widely explored in both field and theoretical studies. The incompatible insect technique (IIT) using Wolbachia, based on cytoplasmic incompatibility, is a technique that ...
The prevalence of Wolbachia in multiple cockroach species and its implication for urban insect management
Tags: Incompatible insect technique, WolbachiaS. O. Oladipupo, Y. Laidoudi, J. F. Beckmann, X. P. Hu and A. G. Appel, Journal of Economic Entomology, 2023.
Cockroach management relies heavily on the use of conventional insecticides in urban settings, which no longer provide the anticipated level of control. Knowledge of cockroach endosymbionts, like Wolbachia, might provide novel avenues for control. Therefore, we screened 16 ...
CRISPR-based gene editing of non-homologous end joining factors biases DNA repair pathway choice toward single-strand annealing in Aedes aegypti
Tags: Aedes, Gene drive, Gene drive synthetic, Genetic biocontrol, Genetically modified organisms, Incompatible insect technique, Replicator/site directed nuclease, Sterile insect technique (SIT)K. Chae, J. M. Overcash, C. Dawson, C. Valentin, H. Tsujimoto, K. M. Myles and Z. N. Adelman, Current Research in Biotechnology, 5:100133. 2023.
To maintain genome stability, eukaryotic cells orchestrate DNA repair pathways to process DNA double-strand breaks (DSBs) that result from diverse developmental or environmental stimuli. Bias in the selection of DSB repair pathways, either non-homologous end joining (NHEJ) or ...
Editorial: Genetic control of insect pest species—achievements, challenges, and perspectives
Tags: Gene drive, Genetic biocontrol, Genetically modified organisms, Incompatible insect technique, Sterile insect technique (SIT)I. Häcker, D. Bartsch, A. Choo and F. Marec, Frontiers in Bioengineering and Biotechnology, 11. 2023.
Genetic control is a type ofbiological control and a promising approach to regulate insect pest populations in a species-specific manner. It is based on targeting the reproductive capacity of the target pest species to reduce population size to non-critical levels. The best known ...
Wolbachia-based strategies for control of agricultural pests
Tags: Agriculture, Cytoplasmic incompatibility, Incompatible insect technique, WolbachiaJ. T. Gong, T. P. Li, M. K. Wang and X. Y. Hong, Curr Opin Insect Sci, 101039:10.1016/j.cois.2023.101039. 2023.
Wolbachia-based incompatible insect technique (IIT) and pathogen blocking technique (PBT) have been shown to be effective at protecting humans from mosquito-borne diseases in the past decades. Population suppression based on IIT and population replacement based on PBT have become ...
The Role of Symbiont-Targeted Strategies in the Management of Pentatomidae and Tephritidae Pests under an Integrated Vision
Tags: Biological control, Incompatible insect technique, Pest management, Sterile insect technique (SIT)E. Gonella and A. Alma, Agronomy, 13. 2023.
The interaction between insects and gut bacterial symbionts is, nowadays, regarded as an important element in the implementation of pest management, in consideration of the urgent need for sustainable alternatives to insecticide use. In this framework, a major tool is symbiotic ...
A bacterium against the tiger: further evidence of the potential of non-inundative releases of males with manipulated Wolbachia infection in reducing fertility of Aedes albopictus field populations in Italy
Tags: Aedes, Incompatible insect technique, Mosquitoes, WolbachiaB. Caputo, R. Moretti, C. Virgillito, M. Manica, E. Lampazzi, G. Lombardi, P. Serini, V. Pichler, N. W. Beebe, A. Della Torre and M. Calvitti, Pest Management Science, 2023.
BACKGROUND: Incompatible Insect Technique (IIT) is a population suppression approach based on the release of males with manipulated Wolbachia infection inducing egg inviability in wild females. We here present results of multiple field releases of incompatible ARwP males carried ...
First report of natural Wolbachia infections in mosquitoes from Cuba
Tags: Incompatible insect technique, Mosquitoes, Other Symbionts, WolbachiaA. Ruiz, G. Gutiérrez-Bugallo, R. Rodríguez-Roche, L. Pérez, R. González-Broche, L. A. Piedra, L. C. Martínez, Z. Menéndez, A. Vega-Rúa and J. A. Bisset, Acta Tropica, 242:106891. 2023.
Mosquitoes are extensively responsible for the transmission of pathogens. Novel strategies using Wolbachia could transform that scenario, since these bacteria manipulate mosquito reproduction, and can confer a pathogen transmission-blocking phenotype in culicids. Here, we ...
Wolbachia pipientis infections in populations of Aedes albopictus in the city of València (Spain): implications for mosquito control
Tags: Aedes, Incompatible insect technique, Population suppression, WolbachiaR. Bueno-Marí, R. Domínguez-Santos, M. Trelis, E. Garrote-Sánchez, M. Cholvi, F. Quero de Lera, M. Khoubbane, A. Marcilla and R. Gi, Revista Española de Salud Pública, 97. 2023.
OBJECTIVE: The presence of Aedes albopictus, of high sanitary and social impact, was first reported in Valencia (Eastern Spain) in 2015. Innovative tools for its control include the use of the endosymbiotic bacterium Wolbachia pipientis. The release of mosquito males infected ...
Satellite Rearing of Aedes Mosquito Eggs: Synchronized Empirical Test of a Novel Mass Rearing Model
Tags: Aedes, Incompatible insect technique, Sterile insect technique (SIT), WolbachiaK. L. Dobson, K. Blore, J. A. Henke, K. Y. Hung, T. Morgan, T. Posey, S. Sun, O. Sypes, N. P. Tremblay and S. L. Dobson, J Am Mosq Control Assoc, 39:12-17. 2023.
Mosquito suppression strategies based on "rear and release" of male mosquitoes are attracting renewed interest from governments, municipalities, and private businesses. These include irradiation-based sterile insect technique, Wolbachia-based technologies, and genetic ...
When less is more: accounting for overcompensation in mosquito SIT projects
Tags: Aedes, Incompatible insect technique, Sterile insect technique (SIT)J. Bouyer, Trends in Parasitology, 2023.
Compensation and overcompensation under field conditions are confirmed in Aedes mosquitoes recently by Evans et al.: equal or increased densities of emerging adults may thus result from reduced larval densities. Here the consequences when applying the sterile insect technique and ...
Joint FAO/IAEA Coordinated Research Project on Mosquito Handling, Transport, Release and Male Trapping Methods in Support of SIT Application to Control Mosquitoes
Tags: Aedes, Genetic biocontrol, Incompatible insect technique, Sterile insect technique (SIT)M. Gómez, B. J. Johnson, H. C. Bossin and R. Argilés-Herrero, Insects, 14. 2023.
The research carried out in the framework of this CRP generated key achievements in areas relevant to the SIT application to control mosquitoes. Among these outcomes, particular mentions must be given to the following: (1) Novel and efficient self-marking techniques were ...
Survival-Larval Density Relationships in the Field and Their Implications for Control of Container-Dwelling Aedes Mosquitoes
Tags: Aedes, Incompatible insect technique, Sterile insect technique (SIT)K. G. Evans, Z. R. Neale, B. Holly, C. C. Canizela and S. A. Juliano, Insects, 14. 2022.
Population density can affect survival, growth, development time, and adult size and fecundity, which are collectively known as density-dependent effects. Container Aedes larvae often attain high densities in nature, and those densities may be reduced when larval control is ...
Assessing the efficacy of male Wolbachia-infected mosquito deployments to reduce dengue incidence in Singapore: study protocol for a cluster-randomized controlled trial
Tags: Aedes, Incompatible insect technique, Sterile insect technique (SIT), WolbachiaJ. Ong, S. H. Ho, S. X. H. Soh, Y. Wong, Y. Ng, K. Vasquez, Y. L. Lai, Y. X. Setoh, C. S. Chong, V. Lee, J. C. C. Wong, C. H. Tan, S. Sim, L. C. Ng and J. T. Lim, Trials, 23:1023. 2022.
The study is designed as a parallel, two-arm, non-blinded cluster-randomized (CR) controlled trial to be conducted in high-rise public housing estates in Singapore, an equatorial city-state. The aim is to determine whether large-scale deployment of male Wolbachia-infected Ae. ...
East Maui project hopes mosquito v. mosquito mating battle will save endangered birds
Tags: Biodiversity/Conservation, Birds, Incompatible insect technique, Invasive species, Malaria, Mosquitoes, Sterile insect technique (SIT)K. Cerizo, MAUINOW, 2022.
A mass rearing cost calculator for the control of Culex quinquefasciatus in Hawaiʻi using the incompatible insect technique
Tags: Culex, Incompatible insect technique, Sterile insect technique (SIT)A. E. Vorsino and Z. Xi, Parasites and Vectors, 15:453. 2022.
Hawaiʻi’s native forest avifauna is experiencing drastic declines due to climate change-induced increases in temperature encroaching on their upper-elevation montane rainforest refugia. Higher temperatures support greater avian malaria infection rates due to greater densities ...
Effect of Wolbachia Infection and Adult Food on the Sexual Signaling of Males of the Mediterranean Fruit Fly Ceratitis capitata
Tags: Fruit fly, Incompatible insect technique, Sterile insect technique (SIT), WolbachiaG. A. Kyritsis, P. Koskinioti, K. Bourtzis and N. T. Papadopoulos, Insects, 13. 2022.
Sexual signaling is a fundamental component of sexual behavior of Ceratitis capitata that highly determines males' mating success. Nutritional status and age are dominant factors known to affect males' signaling performance and define the female decision to accept a male as a ...
Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines
Tags: Aedes, Dengue, Genetic biocontrol, Incompatible insect technique, WolbachiaC. Sadanandane, K. Gunasekaran, D. Panneer, S. K. Subbarao, M. Rahi, B. Vijayakumar, V. Athithan, A. Sakthivel, S. Dinesh and P. Jambulingam, Frontiers in Microbiology, 13:947857. 2022.
Wolbachia, an intracellular maternally transmitted endosymbiont, has been shown to interfere with the replication of dengue virus in Aedes aegypti mosquitoes. The Wolbachia-transinfected Ae. aegypti has been currently released in many countries to test its effectiveness in ...
Wolbachia Dynamics in Mosquitoes with Incomplete CI and Imperfect Maternal Transmission by a DDE System
Tags: Aedes, Incompatible insect technique, Modeling, WolbachiaY. Su, B. Zheng and X. Zou, Bulletin of Mathematical Biology, 84:95. 2022.
In this paper, we propose a delay differential equation model to describe the Wolbachia infection dynamics in mosquitoes in which the key factor of cytoplasmic incompactibility (CI) is incorporated in a more natural way than those in the literature. By analyzing the dynamics of ...
Sensitivity of wMel and wAlbB Wolbachia infections in Aedes aegypti Puducherry (Indian) strains to heat stress during larval development
Tags: Aedes, Asia, Cytoplasmic incompatibility, Incompatible insect technique, WolbachiaK. Gunasekaran, C. Sadanandane, D. Panneer, A. Kumar, M. Rahi, S. Dinesh, B. Vijayakumar, M. Krishnaraja, S. K. Subbarao and P. Jambulingam, Parasites and Vectors, 15:221. 2022.
BACKGROUND: ICMR-Vector Control Research Centre, Puducherry, India, developed two colonies of Aedes aegypti infected with wMel and wAlbB Wolbacia strains called Ae. aegypti (Pud) lines for dengue control. The sensitivity of wMel and wAlbB strains in Ae. aegypti (Pud) lines to ...
Mosquito control to save Hawaiian honeycreepers does not involve GMOs
Tags: Biodiversity/Conservation, Culex, Cytoplasmic incompatibility, Incompatible insect technique, North America, WolbachiaDepartment of Land and Natural Resources, Hawaii Department of Land and Natural Resources, 2022.
Despite misinformation circulating on social media, the importation of “incompatible-male” mosquitoes to control populations of wild mosquitoes and to save four native bird species from extinction, does not involve the use of any genetically modified organisms (GMOs) or ...
Establishment of Wolbachia infection in Aedes aegypti from Pakistan via embryonic microinjection and semi-field evaluation of general fitness of resultant mosquito population
Tags: Aedes, Cytoplasmic incompatibility, Genetic biocontrol, Incompatible insect technique, WolbachiaM. S. Sarwar, N. Jahan, A. Ali, H. K. Yousaf and I. Munzoor, Parasites and Vectors, 15:191. 2022.
BACKGROUND: Dengue is a mosquito-borne viral disease that is mainly spread by Aedes aegypti. It is prevalent on five continents, predominantly in tropical and sub-tropical zones across the world. Wolbachia bacteria have been extensively used in vector control strategies ...
Wolbachia interacts with the microbiome to shape fitness-associated traits during seasonal adaptation in Drosophila melanogaster
Tags: Incompatible insect technique, Other Symbionts, Population modification/replacement, Population suppression, WolbachiaL. P. Henry, M. Fernandez, S. Wolf and J. Ayroles, bioRxiv, 2022.05.31.494239. 2022.
The microbiome contributes to many different host traits, but its role in host adaptation remains enigmatic. The fitness benefits of the microbiome often depend on ecological conditions, but fluctuations in both the microbiome and environment modulate these fitness benefits. ...
Reply to: Assessing the efficiency of Verily’s automated process for production and release of male Wolbachia-infected mosquitoes
Tags: Aedes, Incompatible insect technique, Sterile insect technique (SIT), WolbachiaJ. E. Crawford, K. C. Hopkins, A. Buchman, T. Zha, P. Howell, E. Kakani, J. R. Ohm, N. Snoad, L. Upson, J. Holeman, P. Massaro, S. L. Dobson, F. S. Mulligan and B. J. White, Nature Biotechnology, 2022.
We appreciate the comments from Bouyer et al. under their mandate as a United Nations agency program (‘to promote the safe and appropriate use of nuclear techniques and related technologies in food and agriculture’) on our paper1 . The centuries-old fight against ...
Assessing the efficiency of Verily’s automated process for production and release of male Wolbachia-infected mosquitoes
Tags: Aedes, Incompatible insect technique, Sterile insect technique (SIT), WolbachiaJ. Bouyer, H. Maiga and M. J. B. Vreysen, Nature Biotechnology, 2022.
A paper by Crawford et al.1 titled ‘Efficient production of male Wolbachia-infected Aedes aegypti mosquitoes enables large-scale suppression of wild populations’ reports a mosquito control suppression trial carried out from2017 to 2018 in Fresco Country, California, USA1 . ...
Strategies to Mitigate Establishment under the Wolbachia Incompatible Insect Technique
Tags: Cytoplasmic incompatibility, Genetic biocontrol, Incompatible insect technique, Population suppression, Sterile insect technique (SIT), WolbachiaS. Soh, S. H. Ho, J. Ong, A. Seah, B. S. Dickens, K. W. Tan, J. R. Koo, A. R. Cook, S. Sim, C. H. Tan, L. C. Ng and J. T. Lim, Viruses, 14. 2022.
The Incompatible Insect Technique (IIT) strategy involves the release of male mosquitoes infected with the bacterium Wolbachia. Regular releases of male Wolbachia-infected mosquitoes can lead to the suppression of mosquito populations, thereby reducing the risk of transmission of ...
Pilot trial using mass field-releases of sterile males produced with the incompatible and sterile insect techniques as part of integrated Aedes aegypti control in Mexico
Tags: Aedes, Incompatible insect technique, Mosquito husbandry, Sterile insect technique (SIT), WolbachiaA. Martín-Park, A. Che-Mendoza, Y. Contreras-Perera, S. Pérez-Carrillo, H. Puerta-Guardo, J. Villegas-Chim, G. Guillermo-May, A. Medina-Barreiro, H. Delfín-González, R. Méndez-Vales, S. Vázquez-Narvaez, J. Palacio-Vargas, F. Correa-Morales, G. Ayora-Tal, PLoS Negl Trop Dis, 16:e0010324. 2022.
We implemented a controlled before-and-after quasi-experimental study in two suburban localities of Yucatan (Mexico): San Pedro Chimay (SPC), which received IIT-SIT, and San Antonio Tahdzibichén used as control. Release of wAlbB Ae. aegypti males at SPC extended for 6 months ...
Could species-focused suppression of Aedes aegypti, the yellow fever mosquito, and Aedes albopictus, the tiger mosquito, affect interacting predators? An evidence synthesis from the literature
Tags: Ecology, Incompatible insect technique, Policy, Regulation, Risk and safety, Sterile insect technique (SIT)J. A. S. Bonds, C. M. Collins and L.-C. Gouagna, Pest Management Science, 2022.
The risks of Aedes aegypti and Aedes albopictus nuisance and vector-borne diseases are rising and the adverse effects of broad-spectrum insecticide application has promoted species-specific techniques, such as sterile insect technique (SIT) and other genetic strategies, as ...
Increased biting rate and decreased Wolbachia density in irradiated Aedes mosquitoes
Tags: Aedes, Genetic biocontrol, Incompatible insect technique, Mosquitoes, Sterile insect technique (SIT), WolbachiaR. Moretti, E. Lampazzi, C. Damiani, G. Fabbri, G. Lombardi, C. Pioli, A. Desiderio, A. Serrao and M. Calvitti, Parasites and Vectors, 15:67. 2022.
Releasing considerable numbers of radiation-sterilized males is a promising strategy to suppress mosquito vectors. However, releases may also include small percentages of biting females, which translate to non-negligible numbers when releases are large. Currently, the effects of ...
Uniqueness and stability of periodic solutions for an interactive wild and Wolbachia-infected male mosquito model
Tags: Cytoplasmic incompatibility, Incompatible insect technique, Modeling, Population suppression, WolbachiaR. Yan and Q. Sun, Journal of Biological Dynamics, 2022.
We investigate a mosquito population suppression model, which includes the release of Wolbachia-infected males causing incomplete cytoplasmic incompatibility (CI). The model consists of two sub-equations by considering the density-dependent birth rate of wild mosquitoes. By ...
Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression
Tags: Fruit fly, Genetic biocontrol, Genetic incompatibilities, Incompatible insect technique, Sterile insect technique (SIT)A. Upadhyay, N. R. Feltman, A. Sychla, A. Janzen, S. R. Das, M. Maselko and M. Smanski, eLife, 11. 2022.
Engineered Genetic Incompatibility (EGI) is a method to create species-like barriers to sexual reproduction. It has applications in pest control that mimic Sterile Insect Technique when only EGI males are released. This can be facilitated by introducing conditional ...
Recently introduced Wolbachia reduces bacterial species richness and reshapes bacterial community structure in Nilaparvata lugens
Tags: Incompatible insect technique, Other Symbionts, WolbachiaT.-P. Li, C.-Y. Zhou, J.-T. Gong, Z. Xi and X.-Y. Hong, Pest Management Science, 2022.
BACKGROUND Wolbachia has been developed as an effective tool to suppress insect pests and arbovirus transmission. Recently, the brown planthopper Nilaparvata lugens, a serious agricultural pest, has been successfully transinfected with Wolbachia strain wStri from Laodelphax ...
Lab-scale characterization and semi-field trials of Wolbachia Strain wAlbB in a Taiwan Wolbachia introgressed Ae. aegypti strain
Tags: Dengue, Incompatible insect technique, Mosquito husbandry, Mosquitoes, Population modification/replacement, Population suppression, WolbachiaW. L. Liu, H. Y. Yu, Y. X. Chen, B. Y. Chen, S. N. Leaw, C. H. Lin, M. P. Su, L. S. Tsai, Y. Chen, S. H. Shiao, Z. Y. Xi, A. C. C. Jang and C. H. Chen, PLOS Neglected Tropical Diseases, 16:24. 2022.
Author summaryPrior to open field release, new genetic approaches that interfere with mosquito abilities and reduce mosquito population density require progressive evaluation both in the laboratory and contained field trials. Trials in contained outdoor systems are thus an ...
Intervention of Modern Genetic Tools for Managing Insect Pests of Fruit Crops
Tags: Gene drive synthetic, Genetic biocontrol, Incompatible insect technique, Sterile insect technique (SIT)G. S. Miglani, S. Singh, Z. Li and R. K. Sandhu, Genetic Methods and Tools for Managing Crop Pests, 2022.
Merits and demerits of select modern genetic tools as sterile insect technique, repressible dominant lethal, engineering insect pests, transgenic crops, primary resistance management, gene silencing RNA interference, genome-editing-based methods, autocidal insect control ...
Determinants of stakeholders’ attitudes and intentions toward supporting the use of Wolbachia-infected Aedes mosquitoes for dengue control
Tags: Aedes, Dengue, Incompatible insect technique, Stakeholder engagement, WolbachiaA. F. Arham, L. Amin, M. A. C. Mustapa, Z. Mahadi, M. Yaacob and M. Ibrahim, BMC Public Health, 21:2314. 2021.
BACKGROUND: A recent approach in controlling dengue is by using the Wolbachia-infected Aedes mosquito (WiAM). The approach has been reported to be more effective than traditional methods, such as fogging. Therefore, it is imperative to assess the factors predicting its acceptance ...
Perspectives into Genetic Manipulations for Control of Dengue Vector (Aedes aegypti Linnaeus, 1762) with Reference to Progress in Indian Experiments
Tags: Aedes, Gene drive synthetic, Genetic biocontrol, Incompatible insect technique, Sterile insect technique (SIT)R. Chatterjee, S. Bhattacharya and B. K. Tyagi, Genetically Modified and other Innovative Vector Control Technologies, 2021.
Vector-borne diseases like malaria, dengue, chikungunya, Japanese encephalitis, Zika and others claim millions of lives across the globe annually, and as such their control has become an ardent necessity. Past attempts over the decades have introduced vector control through ...
Wolbachia Endosymbiont and Mosquito Vectors, with Emphasis on Lymphatic Filariasis Elimination
Tags: Culex, Cytoplasmic incompatibility, Incompatible insect technique, Population modification/replacement, WolbachiaI. P. Sunish, Genetically Modified and other Innovative Vector Control Technologies, 2021.
Wolbachia are maternally inherited intracellular bacteria, known to alter early development and mitotic processes in their hosts. They are frequently observed as a reproductive parasite, capable of inducing feminization, parthenogenesis, male killing, or cytoplasmic ...
Field Trials of Gene Drive Mosquitoes: Lessons from Releases of Genetically Sterile Males and Wolbachia-infected Mosquitoes
Tags: Field trials, Gene drive synthetic, Incompatible insect technique, Mosquitoes, Sterile insect technique (SIT), WolbachiaJ. M. Marshall and V. N. Vásquez, Genetically Modified and other Innovative Vector Control Technologies, 2021.
The discovery of CRISPR-based gene editing and its application to homing-based gene drive has been greeted with excitement, for its potential to control mosquito-borne diseases on a wide scale, and concern, for the invasiveness and potential irreversibility of a release. At the ...
Arthropods of Medical Importance: Need for Genetic and Other Innovative Vector Control Technologies, with Emphasis on Eco-biosocial and Environmental Considerations.
Tags: Gene drive synthetic, Genetic biocontrol, Incompatible insect technique, Mosquitoes, Policy, Risk and safety, Stakeholder engagement, Sterile insect technique (SIT), WolbachiaB. K. Tyagi, Genetically Modified and other Innovative Vector Control Technologies, 2021.
Among the world’s known vector groups, viz. arthropods, snails and rodents, the most important vectors originate from arthropods, the jointed legs. Arthropods are doubtlessly regarded as the most dominant creatures on the Earth due largely to their remarkable structural and ...
The Effects of Boric Acid Sugar Bait on Wolbachia Trans-Infected Male Aedes albopictus (ZAP Males®) in Laboratory Conditions
Tags: Genetic biocontrol, Incompatible insect technique, WolbachiaV. S. Aryaprema, W. A. Qualls, K. L. Dobson, S. L. Dobson and R.-D. Xue, Insects, 13. 2021.
The field release of Wolbachia trans-infected male mosquitoes, as well as the use of toxic sugar baits, is a novel and promising candidate technique for integrated mosquito management programs. However, the methods of action of the two techniques may not be complementary, because ...
Genetically Modified and other Innovative Vector Control Technologies
Tags: Gene drive synthetic, Genetic biocontrol, Incompatible insect technique, Mosquitoes, Policy, Risk and safety, Stakeholder engagement, Sterile insect technique (SIT), WolbachiaB. K. Tyagi, SpringerLink, 2021.
This book comprehensively covers the latest development in developing and deploying the genetically modified vectors, particularly Anopheles and Aedes mosquitoes responsible for transmitting malaria parasites and dengue viruses, the most deadly and/or debilitating among all the ...
New molecular genetic techniques: regulatory and societal considerations
Tags: Gene drive synthetic, Genetic biocontrol, Incompatible insect technique, Policy, Regulation, Risk and safety, Risk assessment, Stakeholder engagement, Sterile insect technique (SIT)Nielsen, K. M., AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application, 2021.
A rapidly expanding toolbox of techniques available for genome editing provides the basis for a new continuum in types of modifications that can be introduced into a genome and blur the bimodal GMO vs. non-GMO (genetically modified organism) divide. Site-directed nucleases (SDN) ...
AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application
Tags: Gene drive synthetic, Incompatible insect technique, Sterile insect technique (SIT)Hendrichs, J. Pereira, R., Vreysen, M. J. B., AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application, 2021.
The concept of area-wide integrated pest management (AW-IPM), in which the total population of a pest in an area is targeted, is central to the effective control of such populations through the integration of genetic, biological and other pest suppression technologies. Insect ...
Combined sterile insect technique and incompatible insect technique: concept, study design, experience and lessons learned from a pilot suppression trial in Thailand
Tags: Aedes, Cytoplasmic incompatibility, Incompatible insect technique, Sterile insect technique (SIT), WolbachiaKittayapong, P., AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application, 2021.
Climate change, rapid global transport and land use change leading to urbanization and agricultural intensification have facilitated disease emergence in vulnerable regions like Southeast Asia, and also the global expansion of vectors and vector-borne diseases into other regions ...
Barriers and facilitators of area-wide management including sterile insect technique application: The example of Queensland fruit fly
Tags: Genetic biocontrol, Incompatible insect technique, Policy, Stakeholder engagement, Sterile insect technique (SIT)Mankad, A., Loechel, B., and Measham, P. F., AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application, 2021.
The area-wide management (AWM) of highly mobile insect pests such as tephritid fruit flies requires an integrated understanding of technical, social and institutional processes that drive a coordinated approach within a defined area. Furthermore, the success of an AWM programme ...
Ecology, behaviour and area-wide control of the floodwater mosquito Aedes sticticus, with potential of future integration of the sterile insect technique
Tags: Aedes, Incompatible insect technique, Sterile insect technique (SIT)Lundstrom, J. O. Schafer, M. L. Kittayapong, P., AREA-WIDE INTEGRATED PEST MANAGEMENT: Development and Field Application, 2021.
The strategy of aerial control of the floodwater mosquito Aedes sticticus (Meigen) in the floodplains of River Dalalven, central Sweden, was developed to directly address specific larval breeding areas in temporary flooded wet meadows and swamps. Using the Bti-based larvicide ...
Trial suppresses mosquitoes using non-GMO approach
Tags: Aedes, Genetic biocontrol, Incompatible insect technique, OceaniaGM Watch, GM Watch, 2021.
In a first for the Southern Hemisphere, researchers have shown that a bacterium can successfully suppress populations of the invasive, disease-carrying Aedes aegypti mosquito that is responsible for spreading dengue, yellow fever and Zika. Published in PNAS (see abstract below), ...
Invasive, disease-carrying Aedes aegypti mosquito sterilized with bacteria and eradicated in large-scale trial
Tags: Aedes, Genetic biocontrol, Incompatible insect technique, OceaniaCSIRO, Phys Org, 2021.
In a first for the Southern Hemisphere, researchers have shown a bacteria can successfully sterilize and eradicate the invasive, disease carrying Aedes aegypti mosquito which is responsible for spreading dengue, yellow fever and Zika. The breakthrough could support the ...
Releasing incompatible males drives strong suppression across populations of wild and Wolbachiat-carrying Aedes aegypti in Australia
Tags: Aedes, Genetic biocontrol, Incompatible insect technique, OceaniaN. W. Beebe, D. Pagendam, B. J. Trewin, A. Boomer, M. Bradford, A. Ford, C. Liddington, A. Bondarenco, P. J. De Barro, J. Gilchrist, C. Paton, K. M. Staunton, B. Johnson, A. J. Maynard, G. J. Devine, L. E. Hugo, G. Rasic, H. Cook, P. Massaro, N. Snoad, J., Proceedings of the National Academy of Sciences, 118:e2106828118. 2021.
Through replicated treatment and control experiments in northern Australia, regular releases of Aedes aegypti males infected with a Wolbachia from Aedes albopictus was shown to drive strong population suppression in mosaic populations of wild-type (no Wolbachia) and ...
Wolbachia-mediated sterility suppresses Aedes aegypti populations in the urban tropics
Tags: Aedes, Incompatible insect technique, Population suppression, WolbachiaProject Wolbachia-Singapore Consortium, medRxiv, 2021.
Incompatible insect technique (IIT) via releases of male Wolbachiainfected mosquitoes is a promising tool for dengue control. In a three-year trial in Singaporean high-rise housing estates, we demonstrated that Wolbachia-based IIT dramatically reduces both wildtype Aedes aegypti ...
Modeling and analysis of the implementation of the Wolbachia incompatible and sterile insect technique for mosquito population suppression.
Tags: Dengue, Genetic biocontrol, Incompatible insect technique, Mosquitoes, Population suppression, WolbachiaB. Zheng, J. S. Yu and J. Li, Siam Journal on Applied Mathematics, 81:718-740. 2021.
Mathematical analysis may offer guidance in designing effective mass release strategies for the area-wide application of this Wolbachia incompatible and sterile insect technique in the future. The two most crucial concerns in designing release strategies are how often and in what ...
The Insect Pest Control Laboratory of the Joint FAO/IAEA Programme: Ten Years (2010–2020) of Research and Development, Achievements and Challenges in Support of the Sterile Insect Technique
Tags: Incompatible insect technique, Mosquito husbandry, Sterile insect technique (SIT)M. J. B. Vreysen, A. M. M. Abd-Alla, K. Bourtzis, J. Bouyer, C. Caceres, C. de Beer, D. Oliveira Carvalho, H. Maiga, W. Mamai, K. Nikolouli, H. Yamada and R. Pereira, Insects, 12. 2021.
The Joint FAO/IAEA Centre (formerly called Division) of Nuclear Techniques in Food and Agriculture was established in 1964 and its accompanying laboratories in 1961. One of its subprograms deals with insect pest control, and has the mandate to develop and implement the sterile ...
When More is Less: Mosquito Population Suppression Using Sterile, Incompatible and Genetically Modified Male Mosquitoes
Tags: Cytoplasmic incompatibility, Genetic biocontrol, Incompatible insect technique, Mosquitoes, Sterile insect technique (SIT), WolbachiaS. L. Dobson, Journal of Medical Entomology, 58:1980-1986. 2021.
The current review of the Sterile Insect Technique (SIT) is motivated by new technologies and the recent renaissance of male release field trials, which is driving an evolution in mosquito control and regulation. Practitioners that are releasing male mosquitoes would do well to ...
Number of Project Wolbachia mosquitoes released is constantly reviewed to maintain suppression of dengue: NEA
Tags: Dengue, Genetic biocontrol, Genetic incompatibilities, Incompatible insect technique, WolbachiaN. L. Ching, today, 2021.
Project Wolbachia – Singapore has yielded promising results so far.Releases of non-biting male Wolbachia-Aedes mosquitoes have suppressed the urban Aedes aegypti mosquito populations in study sites at Tampines and Yishun by up to 90 per cent, and we have observed 58 to 74 per ...
GeneConvene Global Collaborative Webinar Series | Genetic Biocontrol 2021
Tags: Genetic biocontrol, Incompatible insect technique, Oxitec, Sterile insect technique (SIT), Webinar, WolbachiaDavid O'Brochta and Hector Quemada, GeneConvene Global Collaborative, 2021.
In the mid 20th century various ideas emerged concerning how genetics and genetic principles could be directly applied to age-old problems of managing insects that threaten food security and public health. This series of webinars will explore the current state-of-the-art of ...
Reply to: Issues with combining incompatible and sterile insect techniques
Tags: Aedes, Genetic biocontrol, Incompatible insect technique, WolbachiaY. Li, L. A. Baton, D. Zhang, J. Bouyer, A. G. Parker, A. A. Hoffmann, L. C. Ng, C. H. Tan and Z. Xi, Nature, 590:E3-E5. 2021.
When the aim is elimination of the target population, Uni-CI and Bi-CI do not have appreciably different risks of population replacement. The small-scale field trial8 cited by Moretti and Calvitti in their Comment1 does not provide evidence that Bi-CI protects against population ...
Issues with combining incompatible and sterile insect techniques
Tags: Aedes, Genetic biocontrol, Incompatible insect technique, WolbachiaR. Moretti and M. Calvitti, Nature, 590:E1-E2. 2021.
In a recent paper, Zheng at al.1 performed field experiments that tested a strategy of mosquito suppression based on the release of functionally sterile males produced by the combination of reproductive unidirectional cytoplasmic incompatibility (which is induced by the bacterium ...
Project Wolbachia: Residents are killing the ‘helpful’ mosquitoes, which can be a nuisance
Tags: Aedes, Cytoplasmic incompatibility, Genetic biocontrol, Incompatible insect technique, WolbachiaT. J. Cheng, today, 2021.
In 2019, Dr Amy Khor, then Senior Minister of State for the Environment and Water Resources, said that there was a 90 per cent suppression rate at study sites in Tampines and Yishun from February to November that year. However, certain public housing estates under the project ...
A patent review on strategies for biological control of mosquito vector
Tags: Biological control, Gene drive, Gene editing, Genetic biocontrol, Genetically modified mosquitoes, Genetically modified organisms, Incompatible insect technique, Mosquitoes, Sterile insect technique (SIT)K. Parihar, M. Telang and A. Ovhal, World Journal of Microbiology and Biotechnology, 36:23. 2020.
This paper presents a comprehensive technology overview of patent documents disclosing biological agents for mosquito control. The patent analysis revealed that comparable number of patent documents were filed in two technology categories: non-recombinant agents and genetically ...
Modelling the Wolbachia incompatible insect technique: strategies for effective mosquito population elimination
Tags: Aedes, Incompatible insect technique, Modeling, Mosquitoes, Population modification/replacement, Population suppression, Sterile insect technique (SIT), WolbachiaD. E. Pagendam, B. J. Trewin, N. Snoad, S. A. Ritchie, A. A. Hoffmann, K. M. Staunton, C. Paton and N. Beebe, BMC Biology, 18:13. 2020.
We introduce a simple Markov population process model for studying mosquito populations subjected to a Wolbachia-IIT programme which exhibit an unstable equilibrium threshold. The model is used to study, in silico, scenarios that are likely to yield a successful elimination ...
Microbiome Innovation in Agriculture: Development of Microbial Based Tools for Insect Pest Management
Tags: Incompatible insect technique, Invasive species, Other Symbionts, WolbachiaM. Qadri, S. Short, K. Gast, J. Hernandez and A. C.-N. Wong, Frontiers in Sustainable Food Systems, 4. 2020.
This review emphasizes the potential and use of microbes in sustainable insect pest management. We first review the diverse insect traits shaped by insect-microbe associations that span nutrition, immunity, ecological interactions with natural enemy, insecticide resistance, and ...
How to fight the deadly dengue virus? Make your own mosquitoes
Tags: Dengue, Genetic biocontrol, Incompatible insect technique, Oxitec, Sterile insect technique (SIT), WolbachiaJ. Emont, Wall Street Journal, 2020.
When the bacteria-laden male mosquitoes are released into the open and mate with naturally-born females, the resultant eggs won’t hatch.
Engineered Reproductively Isolated Species Drive Reversible Population Replacement
Tags: Fruit fly, Gene drive synthetic, Genetic incompatibilities, Incompatible insect technique, Population modification/replacementA. Buchman, I. Shriner, T. Yang, J. Liu, I. Antoshechkin, J. M. Marshall, M. W. Perry and O. S. Akbari, bioRxiv, 2020.08.09.242982. 2020.
Engineered reproductive species barriers are useful for impeding gene flow and driving desirable genes into wild populations in a reversible threshold-dependent manner. We engineer multiple reproductively isolated SPECIES and demonstrate their threshold-dependent gene drive ...
Genetic Biocontrol – An Overview (video 13.13 min)
Tags: Cytoplasmic incompatibility, Gene drive synthetic, Genetic biocontrol, Incompatible insect technique, Sterile insect technique (SIT), Toxin-antidote, Underdominance, Wolbachia, X chromosomeGeneConvene Global Collaborative, 2020.
This video explains what genetic biocontrol is and surveys various technologies that can be consider genetic biocontrol technologies. It offers a conceptual organization of the various technologies based on the potential of genetic biocontrol organisms to persist and spread in ...
Can we kill the dreaded mosquito? Do we even want to?
Tags: Dengue, Gene drive synthetic, Genetic biocontrol, Incompatible insect technique, Malaria, Mosquitoes, Sterile insect technique (SIT), WolbachiaStacey McKenna, Sierra, 2020.
As a major vector for disease, the mosquito has harmed more human beings than just about any other animal, and a changing climate is only boosting those numbers. As the range of disease-carrying species of mosquitoes expands, so does their ability to transmit the parasites and ...
Efficient production of male Wolbachia-infected Aedes aegypti mosquitoes enables large-scale suppression of wild populations
Tags: Aedes, Incompatible insect technique, Sterile insect technique (SIT), WolbachiaJ. E. Crawford, D. W. Clarke, V. Criswell, M. Desnoyer, D. Cornel, B. Deegan, K. Gong, K. C. Hopkins, P. Howell, et al., Nature Biotechnology, 38:482-492. 2020.
The range of the mosquito Aedes aegypti continues to expand, putting more than two billion people at risk of arboviral infection. The sterile insect technique (SIT) has been used to successfully combat agricultural pests at large scale, but not mosquitoes, mainly because of ...
The value of existing regulatory frameworks for the environmental risk assessment of agricultural pest control using gene drive
Tags: Biological control, Gene drive synthetic, Genetically modified organisms, Incompatible insect technique, Regulation, Risk assessment, Sterile insect technique (SIT)J. Romeis, J. Collatz, D. C. M. Glandorf and M. B. Bonsall, Environmental Science & Policy, 108:19-36. 2020.
The application of (synthetic) gene drives is a powerful tool to control populations of insects that are agricultural pests, vectors of diseases, or a threat to biodiversity potentially leading to the local or global eradication of a species. The potential use of gene drive ...
Wolbachia transinfections in Culex quinquefasciatus generate cytoplasmic incompatibility
Tags: Culex, Cytoplasmic incompatibility, Incompatible insect technique, Population modification/replacement, WolbachiaT. H. Ant, C. Herd, F. Louis, A. B. Failloux and S. P. Sinkins, Insect Molecular Biology, 29:1-8. 2020.
Culex quinquefasciatus is an important mosquito vector of a number of viral and protozoan pathogens of humans and animals, and naturally carries the endosymbiont Wolbachia pipientis, strain wPip. Wolbachia are used in two distinct vector control strategies: firstly, population ...
Incompatible and sterile insect techniques combined eliminate mosquitoes
Tags: Aedes, Genetic biocontrol, Incompatible insect technique, WolbachiaX. Zheng, D. Zhang, Y. Li, C. Yang, Y. Wu, X. Liang, Y. Liang, X. Pan, L. Hu, Q. Sun, X. Wang, Y. Wei, J. Zhu, W. Qian, Z. Yan, A. G. Parker, J. R. L. Gilles, K. Bourtzis, J. Bouyer, M. Tang, B. Zheng, J. Yu, J. Liu, J. Zhuang, Z. Hu, M. Zhang, J.-T. Gon, 572, 56-61. 2019.
Here we show that combining incompatible and sterile insect techniques (IIT–SIT) enables near elimination of field populations of the world’s most invasive mosquito species, Aedes albopictus. Millions of factory-reared adult males with an artificial triple-Wolbachia infection ...
SCIENTIFIC OPINION: In response to the referral of 12 October 2015 concerning use of genetically modified mosquitoes for vector control
Tags: Gene drive synthetic, Genetic biocontrol, Genetically modified organisms, Incompatible insect technique, Oxitec, Policy, Regulation, Risk and safety, WolbachiaHigh Council for Biotechnology, High Council for Biotechnology (France), 2017.
The Scientific Committee’s opinion describes emerging vector control techniques using GM mosquitoes, the current state of research into and development of these techniques and the outcomes of initial experiments worldwide. To date, only one technique has been developed to an ...
